Mutli-functional photometer with movable linkage for routing optical fibers

• US 5,436,718 A
• Filed: 07/30/1993
• Issued: 07/25/1995
• Est. Priority Date: 07/30/1993
• Status: Expired due to Fees

• Current Assignee: GE Healthcare SV Corp (Thermo Fisher Scientific Incorporated)
• Original Assignee: Biolumin Corporation
• Inventors: Norris, Michael C., Fernandes, Jorge
• Primary Examiner(s): EVANS, FANNIE L
• Application Number:
  US08/100,541
• Time in Patent Office: 725 Days
• Field of Search: 356/73, 356/317, 356/318, 356/319, 356/326, 356/328, 356/402, 356/409, 356/410, 356/414-419, 356/432, 356/434-436, 356/440, 250/227.23, 250/227.26, 250/458.1, 250/459.1, 250/461.1, 250/461.2, 385/15, 385/16, 385/18, 385/19, 385/25
• US Class Current: 356/73
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G02B 6/2804 forming multipart couplers without wavelength selective elements, e.g. "T" couplers, star couplers
• CPC Class Codes: G02B 6/3624 Fibre head, e.g. fibre probe termination optical details of light guide terminations G02B6/241; reshaping of light guides G02B6/2552; optical details of coupling light into or out of fibre end G02B6/262
• CPC Class Codes: G02B 6/3644 the coupling means being through-holes or wall apertures
• CPC Class Codes: G02B 6/3897 Connectors fixed to housings, casing, frames or circuit boards G02B6/44528 takes precedence

Abstract

A multi-functional photometer includes a scanning mechanism having a housing (10) that bears a movable linkage (12). The linkage is coupled to an optical scanning head (18) and incorporates optical fibers for transmitting radiant energy to and from the scanning head. The arm comprises a C-shaped "elbow" member (14), pivotally attached to a "shoulder" member (16). In turn, the "shoulder" member of the arm is pivotally connected to the housing. Dynamic couplings join the optical fibers such that the shapes thereof remain fixed regardless of the orientation of the arm. The housing further incorporates a Cartesian-coordinate table (20) for positioning the scanning head with respect to a microplate (22) that contains a plurality of analyte samples.

Claims

1. A photometer utilizing light-transmitting conduits for analyzing optical properties of at least one analyte sample, said photometer comprising:

an optical system having a plurality of lenses; and

a scanner for reading said at least one analyte sample, said scanner being optically coupled with said optical system by said light-transmitting conduits having fixed individual shapes, said scanner including a linkage supportingly routing said light-transmitting conduits and maintaining said fixed individual shapes of said light-transmitting conduits as said scanner scans said at least one analyte sample.

2. The photometer of claim 1 further including a logic for controlling said scanner and said optical system.

3. The photometer of claim 2 wherein said logic comprises a computer.

4. The photometer of claim 1 wherein said scanner further comprises:

a housing capable of supporting a structure for holding said at least one analyte sample;

a positioning device coupled to said housing; and

a first optical scanning element coupled to said positioning device, said first optical scanning element optically coupled with said optical system by a first arrangement of said light-transmitting conduits, said positioning device capable of imparting scanning movements to said first optical scanning element in order to scan said at least one analyte sample.

5. The photometer of claim 4 wherein said positioning device is a Cartesian-coordinate positioning table controlled by a computer.

6. The photometer of claim 4 wherein said linkage is a movable arm comprising:

a first rigid member pivotally attached to said housing at a first axis;

a second rigid member pivotally attached to said first rigid member at a second axis and having a first beam, said first optical scanning element attached to said first beam;

couplings for optically and pivotally interconnecting said light-transmitting conduits, said couplings disposed along said first and said second axes on said first and said second rigid members.

7. The photometer of claim 6 wherein said second rigid member further includes a second beam, said scanner further comprising a second optical scanning element coupled to said second beam, said first and said second optical scanning elements sharing an optical axis.

8. The photometer of claim 7 wherein said second optical scanning element comprises a photodetector.

9. The photometer of claim 7 wherein said second optical scanning element is optically coupled to a light-dispersing device by a second arrangement of said light-transmitting conduits having fixed individual shapes, said second arrangement of said light-transmitting conduits supported by said moveable arm and optically interconnected by said couplings.

10. The photometer of claim 9 wherein said light-dispersing device comprises a diffraction grating.

11. The photometer of claim 9 wherein said second optical scanning element comprises a lens.

12. The photometer of claim 6 wherein said couplings comprise conduit couplers arranged in pairs, each of said conduit couplers having a through aperture, each of said through apertures housing an end of one of said light-transmitting conduits, the through apertures of each said pairs of conduit couplers being collinear and optically interconnecting the ends of the light-transmitting conduits inserted therein.

13. The photometer of claim 12 wherein each of said conduit couplers has a symmetry axis, is substantially cylindrical in shape, and is made of an opaque material, the through aperture of each said conduit coupler being cylindrical.

14. The photometer of claim 12 wherein said couplings further include at least one coupling comprising one of said conduit couplers paired with a conduit integrator having a through aperture that houses ends of a plurality of said light-transmitting conduits contiguous with a segment of light-transmitting conduit, said through apertures of said conduit integrator and said conduit coupler being collinear, said segment of light-transmitting conduit optically coupling said ends of said light-transmitting conduits with the end of the light-transmitting conduit inserted into the through aperture of said conduit coupler.

15. The photometer of claim 14 wherein said plurality of light-transmitting conduits is three.

16. The photometer of claim 14 wherein said conduit integrator has a symmetry axis, a substantially cylindrical shape, and is made of an opaque material, said through aperture of said conduit integrator having a substantially cylindrical shape.

17. The photometer of claim 4 wherein said first optical scanning element comprises a body having a through bore and a plurality of through cavities surrounding said through bore, said through bore housing at least one of said light-transmitting conduits, said plurality of through cavities accommodating a corresponding plurality of said light-transmitting conduits.

18. The photometer of claim 17 wherein each of said plurality of through cavities is formed obliquely with respect to said symmetry axis.

19. The photometer of claim 17 wherein said plurality of through cavities is three.

20. The photometer of claim 17 wherein said body is made of an opaque material, said body having a substantially cylindrical shape and a symmetry axis, said through bore further housing a lens.

21. The photometer of claim 1 wherein said optical system further includes:

a light source; and

a power supply for energizing said light source, said power supply being controlled by a computer.

22. The photometer of claim 21 wherein said light-source is a xenon arc lamp and said power supply is a direct-current power supply.

23. The photometer of claim 1 wherein said light-transmitting conduits comprise optical fibers.

24. The photometer of claim 23 wherein said optical fibers are made of quartz.

25. The photometer of claim 1 wherein said optical properties are at least one of absorbance, fluorescence, and luminescence.

26. A photometer for measuring optical properties of at least one analyte sample, said photometer comprising:

an optical system having a plurality of lenses; and

a scanning mechanism comprising;

a housing capable of supporting a structure for holding said at least one analyte sample;

a positioning device coupled to said housing;

a first optical scanning element coupled to said positioning device, said first optical scanning element being optically coupled to said optical system by a plurality of optical fibers, said positioning device capable of imparting scanning movements to said first optical scanning element in order to scan said at least one analyte sample, said plurality of optical fibers having fixed individual shapes; and

a movable linkage coupled to said housing and to said first optical scanning element, said movable linkage supportingly routing said plurality of optical fibers for maintaining said fixed individual shapes thereof as said first optical scanning element scans said at least one analyte sample.

27. The photometer of claim 26 further including a microprocessor for controlling said positioning device and for monitoring said optical system.

28. The photometer of claim 26 wherein said plurality of optical fibers includes an input elbow fiber, an input shoulder fiber, an input housing fiber, an array of pick-up elbow fibers, a pick-up shoulder fiber, and a pick-up housing fiber, each of said plurality of optical fibers having a distal end and a proximal end.

29. The photometer of claim 28 wherein said movable linkage comprises:

a rigid shoulder member pivotally attached to said housing at a first axis, said housing bearing said input and pick-up housing fibers, said rigid shoulder member bearing said input and pick-up shoulder fibers;

a rigid elbow member pivotally attached to said rigid shoulder member at a second axis and having a first beam, said first optical scanning element attached to said first beam, said rigid elbow member bearing said input elbow fiber and said array of pick-up elbow fibers, said distal end of said input elbow fiber located in a through bore of said first optical scanning element, said distal ends of said array of pick-up elbow fibers located in through cavities surrounding said through bore;

a first optical input coupling arranged collinearly with said first axis, said first optical input coupling optically interconnecting said input housing fiber and said input shoulder fiber by providing a linear light path therebetween;

a first optical pick-up coupling arranged collinearly with said first axis, said first optical pick-up coupling optically interconnecting said pick-up housing fiber and said pick-up shoulder fiber by providing a linear light path therebetween;

a second optical input coupling arranged collinearly with said second axis, said second optical input coupling optically interconnecting said input shoulder fiber and said input elbow fiber by providing a linear light path therebetween; and

a second optical pick-up coupling arranged collinearly with said second axis, said second optical pick-up coupling optically interconnecting said pick-up shoulder fiber and said array of pick-up elbow fibers by providing a linear light path therebetween.

30. The photometer of claim 29 wherein:

said first optical input coupling comprises an input housing coupler and a first input shoulder coupler each having a through aperture collinear with said first axis, said input housing coupler rigidly attached to said housing and accommodating the distal end of said input housing fiber in its through aperture, said first input shoulder coupler rigidly attached to said shoulder member and accommodating the proximal end of said input shoulder fiber in its through aperture, said input housing and first input shoulder couplers having facing surfaces separated by a gap to allow said shoulder member to pivot with respect to said housing;

said first optical pick-up coupling comprises a pick-up housing coupler and a first pick-up shoulder coupler each having a through aperture collinear with said first axis, said pick-up housing coupler rigidly attached to said housing and accommodating the distal end of said pick-up housing fiber in its through aperture, said first pick-up shoulder coupler rigidly attached to said shoulder member and accommodating the proximal end of said pick-up shoulder fiber in its through aperture, said pick-up housing and first pick-up shoulder couplers having facing surfaces separated by a gap to allow said shoulder member to pivot with respect to said housing;

said second optical input coupling comprises a second input shoulder coupler and an input elbow coupler each having a through aperture collinear with said second axis, said input shoulder coupler rigidly attached to said shoulder member and accommodating the distal end of said input shoulder fiber in its through aperture, said input elbow coupler rigidly attached to said elbow member and accommodating the proximal end of said pick-up elbow fiber in its through aperture, said second input shoulder and input elbow couplers having facing surfaces separated by a gap to allow said elbow member to pivot with respect to said shoulder member;

said second optical pick-up coupling comprises a second pick-up shoulder coupler and a pick-up fiber integrator each having a through aperture collinear with said second axis, said second pick-up shoulder coupler being rigidly attached to said shoulder member and accommodating the distal end of said pick-up shoulder fiber in its through aperture, said pick-up fiber integrator rigidly attached to said elbow member and accommodating the proximal ends of said array of pick-up elbow fibers contiguously arranged with an optical fiber segment in its through aperture, said second pick-up shoulder coupler and said pick-up fiber integrator having facing surfaces separated by a gap to allow said elbow member to pivot with respect to said shoulder member.

31. The photometer of claim 29 wherein said rigid elbow member further includes a second beam bearing a second optical scanning element, said first and second optical scanning elements sharing an optical axis.

32. The photometer of claim 31 wherein said second optical scanning element comprises a photodetector.

33. The photometer of claim 31 wherein said second optical scanning element is optically coupled to a light-dispersing device by a second plurality of optical fibers.

34. The photometer of claim 33 wherein said second optical scanning element comprises a lens.

35. The photometer of claim 33 wherein said second plurality of optical fibers comprises:

an optical housing fiber supported by said housing and having a fixed shape;

an optical shoulder fiber supported by said shoulder member and having a fixed shape, said optical shoulder fiber optically interconnected with said optical housing fiber by a first optical coupling, said first optical coupling providing a linear light path between said optical housing fiber and said optical shoulder fiber, said first optical coupling arranged collinearly with said first axis; and

an optical elbow fiber supported by said elbow member and having a fixed shape, said optical elbow fiber optically interconnected with said optical shoulder fiber by a second optical coupling, said second optical coupling providing a linear light path between said optical shoulder fiber and said optical elbow fiber, said second optical coupling arranged collinearly with said second axis.

36. The photometer of claim 35 wherein:

said first optical coupling comprises a housing coupler and a first shoulder coupler each having a central through aperture collinear with said first axis, said housing coupler rigidly attached to said housing and accommodating the distal end of said optical housing fiber in its central through aperture, said first shoulder coupler rigidly attached to said shoulder member and accommodating the proximal end of said optical shoulder fiber in its central through aperture, said housing and first shoulder couplers having facing surfaces separated by a gap to allow said shoulder member to pivot with respect to said housing; and

said second optical coupling comprises a second shoulder coupler and an elbow coupler each having a central through aperture collinear with said second axis, said second shoulder coupler rigidly attached to said shoulder member and accommodating the distal end of said optical shoulder fiber in its central through aperture, said elbow coupler rigidly attached to said elbow member and accommodating the proximal end of said optical elbow fiber in its central through aperture, said second shoulder and elbow couplers having facing surfaces separated by a gap to allow said elbow member to pivot with respect to said shoulder member.

37. The photometer of claim 26 wherein said optical system further includes a light source energized by a computer-controlled power supply.

38. The photometer of claim 37 wherein said light source is a xenon arc lamp.

39. The photometer of claim 26 wherein said optical properties are at least one of absorbance, fluorescence, and luminescence.

40. The photometer of claim 26 wherein said positioning device comprises a Cartesian-coordinate positioning table.

41. The photometer of claim 26 wherein said first optical scanning element comprises a body having a symmetry axis, a through bore, and through openings surrounding said through bore.

42. The photometer of claim 41 wherein said body has a substantially cylindrical shape, said through bore housing a lens, said through openings being equidistant from each other and from said symmetry axis, said through openings being formed obliquely with respect to said symmetry axis.

43. A photometer utilizing light-transmitting paths for analyzing optical properties of at least one analyte sample, said photometer comprising:

an optical system; and

a scanner for reading said at least one analyte sample, said scanner being optically coupled with said optical system by said light-transmitting paths having fixed individual shapes, said scanner including a moveable arm supportingly routing said light-transmitting paths and maintaining said fixed individual shapes of said light-transmitting paths as said scanner scans said at least one analyte sample.

44. The photometer of claim 43 wherein said scanner further comprises:

a housing capable of supporting a structure for holding said at least one analyte sample;

a positioning device; and

a first optical scanning element coupled to said positioning device, said first optical scanning element optically coupled with said optical system by a first arrangement of said light-transmitting paths, said positioning device capable of imparting scanning movements to said first optical scanning element in order to scan said at least one analyte sample.

45. The photometer of claim 44 wherein said linkage is a moveable arm comprising:

a first rigid member pivotally attached to said housing at a first axis;

a second rigid member pivotally attached to said first rigid member at a second axis and having a first beam, said optical scanning element attached to said first beam;

couplings for optically and pivotally interconnecting said light-transmitting paths, said couplings disposed along said first and said second axes on said first and said second rigid members.

46. The photometer of claim 45 wherein said second rigid member further includes a second beam, said scanner further comprising a second optical scanning element coupled to said second beam, said first and said second optical scanning elements sharing an optical axis.

47. The photometer of claim 46 wherein said second optical scanning element is optically coupled to a light-dispersing device by a second arrangement of said light-transmitting paths having fixed individual shapes, said second arrangement of said light-transmitting paths supported by said moveable arm and optically interconnected by said couplings.