Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
First Claim
1. A method for obtaining ex vivo human stem cell division comprising culturing a human hematopoietic stem cell composition in a liquid culture medium which is replaced at a rate of from 50 to 100% daily replacement, while maintaining said culture under physiologically acceptable conditions.
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Abstract
Methods, including culture media conditions, which provide for ex vivo human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
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Citations
53 Claims
- 1. A method for obtaining ex vivo human stem cell division comprising culturing a human hematopoietic stem cell composition in a liquid culture medium which is replaced at a rate of from 50 to 100% daily replacement, while maintaining said culture under physiologically acceptable conditions.
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22. A method for expanding and harvesting a human hematopoietic stem cell pool, comprising:
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(i) culturing a human hematopoietic stem cell composition in a liquid culture medium which is replaced at a rate from 50 to 100% daily replacement, while maintaining said culture under physiologically acceptable conditions, to obtain an expanded human hematopoietic stem cell pool; and (ii) harvesting said expanded human hematopoietic stem cell pool. - View Dependent Claims (52)
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- 23. A method for culturing a human hematopoietic progenitor cell composition comprising culturing human hematopoietic progenitor cells in a liquid culture medium which is replaced at a rate from 50 to 100% daily replacement, while maintaining said culture under physiologically acceptable conditions.
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