×

Cell-associated glucosyltransferase, an antibody thereto, and a dental caries prophylactic composition containing said antibody as an effective component

  • US 5,439,680 A
  • Filed: 12/02/1994
  • Issued: 08/08/1995
  • Est. Priority Date: 01/22/1988
  • Status: Expired due to Term
First Claim
Patent Images

1. A composition for topical use to prevent adherence of Streptococcus mutans cells on tooth surfaces, which comprises:

  • (i) an effective amount of an egg yolk antibody obtained by the steps of;

    (A) cultivating cells of Streptococcus mutans serotype c, e, or f in a nutrient-containing culture medium to obtain a culture containing cultured cells;

    (B) removing the supernatant from the culture to recover the cultured cells;

    (C) extracting a glucosyltransferase from the cultured cells using a urea or guanidine-HCl solution and purifying the glucosyltransferase, said purified glucosyltransferase having the following physicochemical characteristics;

    (a) capable of catalyzing the synthesis of water-insoluble glucan from sucrose;

    (b) having an optimum pH between 6.7 and 7.0 for catalyzing the synthesis of water-insoluble glucan from sucrose;

    (c) losing its activity by heat treatment at 80°

    C. for 5 minutes;

    (d) having a molecular weight of 150 to 165 kilodaltons as determined by SDS-polyacrylamide gel electrophoresis; and

    (e) antigenic in animals and capable of raising an antibody specifically binding to the glucosyltransferase;

    (D) immunizing hens with the purified glucosyltransferase; and

    (E) separating the egg yolk antibody, which specifically binds to the glucosyltransferase, from the eggs of the immunized hens; and

    (ii) a physiologically acceptable diluent or carrier.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×