Chemical analysis and imaging by discrete fourier transform spectroscopy

• US 5,440,388 A
• Filed: 08/02/1993
• Issued: 08/08/1995
• Est. Priority Date: 08/02/1993
• Status: Expired due to Fees

• Current Assignee: John W. Erickson
• Original Assignee: John W. Erickson
• Inventors: Erickson, Jon W.
• Primary Examiner(s): Turner, Samuel A.
• Assistant Examiner(s): KIM, ROBERT H
• Application Number:
  US08/101,389
• Time in Patent Office: 736 Days
• Field of Search: 356/345, 356/346, 356/349, 250/553, 250/578.1
• US Class Current: 356/456
• CPC Class Codes: A61B 5/7203 for noise prevention, reduction or removal
• CPC Class Codes: A61B 5/7257 using Fourier transforms
• CPC Class Codes: G01J 3/4535 Devices with moving mirror G01J3/4532 takes precedence
• CPC Class Codes: G01N 2021/178 Methods for obtaining spatial resolution of the property being measured
• CPC Class Codes: G01N 2021/3595 using FTIR
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B
• CPC Class Codes: G01N 2201/0625 Modulated LED
• CPC Class Codes: G01N 2201/129 Using chemometrical methods

Abstract

An instrument for chemical spectroscopy with imaging capabilities. A lightsource produces an array of light beams, each of which is made up of a plurality of discrete wavelengths. The array of light beams are modulated by an interferometer, then directed through a sample to an array of detectors. The sample may be a chemical mixture (e.g. a fuel stream in a manufacturing facility) or a body part (e.g. breast, limb, or head). An array of laser or light-emitting diodes provides light at the desired wavelengths and high intensity. The set of wavelengths is selected for a particular kind of analysis, and a specific set of possible absorbing species to be detected. The different wavelengths are guided optically (using fiber optics, lenses, and/or mirrors) into a single lightbeam, or an array of lightbeams. This light is then directed through the sample and onto a detector. The lightsource and detector, or lightsource alone, may be rastered if necessary to form an image. Individual lightbeams in an array may be modulated, polarized, or both so as to improve resolution. The signal from the detector undergoes a Fast Fourier Transform to produce a near-infrared absorption spectrum as a function of wavelength. The absorption spectra can be used to produce an image of the spacial distribution of detected species within the sample. Either the lightsource or detectors can be placed on the end of a probe or catheter for imaging through the wall of a hollow sample.

Claims

1. An optical sensor comprising:

a plurality of monochromatic lightsources, each of said monochromatic lightsources producing light at a discrete wavelength, means for combining the light from said plurality of monochromatic lightsources into at least one light beam, said at least one light beam comprising a plurality of discrete wavelengths of light,

an interferometer means for modulating said at least one light beam from said light source,

means for directing said at least one light beam through a sample,

a detector having means for detecting each of said plurality of discrete wavelengths of light in said at least one light beam after said at least one light beam has passed through said sample, said detector producing a detector signal indicative of the intensity of each of said plurality of discrete wavelengths of light in the detected light beam,

and a signal processing means for analyzing said detector signal.

2. The optical sensor of claim 1 wherein said signal processing means performs a Fourier transform on said detector signal.

3. The optical sensor of claim 1 wherein said signal processing means performs a fast Fourier transform on said detector signal.

4. The optical sensor of claim 1 wherein said interferometer means comprises a beam splitter which splits said at least one light beam into a first light path and a second light path, said first light path having a fixed pathlength, said second light path having a pathlength which is varied cyclically, and a means for recombining said first light path and said second light path.

5. The optical sensor of claim 1 wherein said signal processing means analyzes the composition of said sample based on the absorption of each of said plurality of discrete wavelengths of light in said at least one light beam by said sample.

6. The optical sensor of claim 1 wherein said means for combining the light from said plurality of monochromatic lightsources into at least one light beam Combines the light from said plurality of monochromatic lightsources to produce a two dimensional array of light beams, each of the light beams in said two dimensional array of light beams comprising a plurality of discrete wavelengths of light.

7. The optical sensor of claim 6 wherein said signal processing means forms an image of said sample based on the absorption of each of said plurality of discrete . . . .wavelengths of light in each of the light beams in said two dimensional array of light beams by said Sample.

8. The optical sensor of claim 6 wherein said signal processing means analyzes the composition of said sample based on the absorption of each of said plurality of discrete wavelengths of light in each of the light beam in said two dimensional array of light beams by said sample and forms an image representing the distribution of the composition of said sample.

9. The optical sensor of claim 6 wherein said means for combining the light from said plurality of monochromatic lightsources produces said two dimensional array of light beams by combining the light from said plurality of monochromatic lightsources into a single light beam comprising a plurality of discrete wavelengths of light and rastering said single light beam in two dimensions.

10. The optical sensor of claim 6 wherein said means for combining the light from said plurality of monochromatic lightsources produces said two dimensional array of light beams by combining the light from said plurality of monochromatic lightsources into linear array of light beams, each of the light beams in said linear array of light beams comprising a plurality of discrete wavelengths of light and rastering said linear array of light beams in one dimension.

11. The optical sensor of claim 6 further comprising a modulation means for improving the signal-to-noise ratio of said optical sensor wherein said modulation means comprises a means for selecting photon from said at least one light beam having the shortest, direct pathlength from said lightsource means from said detector means and for rejecting photons having a longer, less direct pathlength from said lightsource means to said detector means.

12. The optical sensor of claim 6 further comprising a modulation means for improving the signal-to-noise ratio of said optical sensor wherein said modulation means comprises a means for selecting photons from said at least one light beam having a given pathlength from said lightsource means to said detector means.

13. The optical sensor of claim 12 wherein said modulation means comprises a phase locked loop for selecting photons from said at least one light beam having a given pathlength from said lightsource means to said detector means.

14. The optical sensor of claim 12 wherein said modulation means comprises a gating circuit for selecting photons from said at least one light beam having a given pathlength from said lightsource means to said detector means.

15. The optical sensor of claim 1 further comprising a collimator for eliminating scattered and off-axis light from said at least one light beam after it has passed through said sample and before it is detected by said detector means.

16. The optical sensor of claim 1 wherein said sample comprises a biological tissue.

17. The optical sensor of claim 16 further comprising a compression means for compressing said biological tissue to alter the pathlength of said at least one light beam through said biological tissue.

18. The optical sensor of claim 16 wherein said optical sensor analyzes metabolites within said biological tissue.

19. The optical sensor of claim 1 wherein said means for directing said at least one light beam through a sample comprises a plurality of fiber-optic waveguides mounted on an elongated probe means for insertion into a hollow sample and means for directing light transmitted by said plurality of fiber-optic waveguides through a wall of said hollow sample, said mean for combining the light from said plurality of monochromatic lightsources into at least one light beam comprising means for combining the light from said plurality of monochromatic lightsources into a plurality of lightbeams and means for directing each of said plurality of light beams into a separate fiber-optic wave guide in said plurality of fiber-optic waveguides, each of said plurality of light beams comprising a plurality of discrete wavelengths of light, and said detector being configured to detect the light transmitted through said wall of said hollow sample from said plurality of fiber-optic waveguide.

20. An optical sensor comprising:

a plurality of monochromatic lightsource each of said monochromatic lightsources producing light at a discrete wavelength, means for combining the light from said plurality of monochromatic light sources into a two dimensional array of light beams, each of the light beams in said two dimensional array of light beams comprising a plurality of discrete wavelengths of light,

an interferometer means for modulating said array of light beams from said light source,

means for directing said array of light beams through a sample,

a detector having means for detecting each of said plurality of discrete wavelengths of light in said array of light beams after said array of light beams has passed through said sample, said detector comprising a two dimensional array of broadband light detectors, said detector producing a detector signal indicative of the intensity of each of said plurality of discrete wavelengths of light in the detected array of light beams,

and a signal processing means for analyzing said detector signal.

21. The optical sensor of claim 20 wherein said signal processing means analyzes the composition of said sample based on the absorption of each of said plurality of discrete wavelengths of light in said array of light beams by said sample and forms an image representing the distribution of the composition of said sample.

22. The optical sensor of claim 20 further comprising a switching means for improving the signal-to-noise ratio of said optical sensor, said switching means dividing said light beams of said two dimensional array into at least two groups and activating said at least two groups alternately.

23. The optical sensor of claim 22 wherein said switching means divides said light beams of said two dimensional array into two groups such that light beams which are nearest neighbors in said two dimensional array are in different groups and activating said two groups alternately.

24. The optical sensor of claim 22 wherein said switching means divides said light beams of said two dimensional array into three groups such that light beams which are nearest neighbors and which are next nearest neighbors in said two dimensional array are in different groups and activating said three groups alternately.

25. The optical sensor of claim 20 wherein at least one of said means for directing said array of light beams through a sample or said detector means is mounted on an elongated probe means for insertion into a hollow sample and wherein said means for directing said array of light beams through a sample directs said array of light beams through a wall of said hollow sample.

26. An optical sensor comprising:

a plurality of monochromatic lightsources, each of said monochromatic lightsources producing light at a discrete wavelength, means for combining the light from said plurality of monochromatic lightsources into a linear array of light beams, each of the light beams in said linear array of light beams comprising a plurality of discrete wavelengths of light,

an interferometer means for modulating said linear array of light beams from said light source,

means for directing said linear array of light beams through a sample,

a detector having means for detecting each of said plurality of discrete wavelengths of light in said linear array of light beams after said linear array of light beams has passed through said sample, said detector producing a detector signal indicative of the intensity of each of said plurality of discrete wavelengths of light in the detected light beam,

and a signal processing means for analyzing said detector signal.

27. The optical sensor of claim 26 wherein at least one of said means for directing said linear array of light beams through a sample or said detector is mounted on an elongated probe means for insertion into a hollow sample and wherein said means for directing said linear array of light beams through a sample directs said linear array of light beams through a wall of said hollow sample.