Apparatus for measuring absorption information in scattering medium and method therefor

• US 5,441,054 A
• Filed: 07/19/1993
• Issued: 08/15/1995
• Est. Priority Date: 07/20/1992
• Status: Expired due to Term

• Current Assignee: Hamamatsu Photonics KK
• Original Assignee: Hamamatsu Photonics KK
• Inventors: Tsuchiya, Yutaka
• Primary Examiner(s): Cohen, Lee S.
• Assistant Examiner(s): Casler, Brian L.
• Application Number:
  US08/092,996
• Time in Patent Office: 757 Days
• Field of Search: 128/664, 128/665, 128/633, 356/432, 356/433, 356/39, 250/339.12, 250/341
• US Class Current: 600/310
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

Modulated light from a light source is incident on the surface of a scattering medium, and light passing through this scattering medium is externally detected. A signal of a predetermined frequency component is extracted from this detection signal to detect a photon density wave propagating in the scattering medium. A signal extracted to correspond to this photon density wave is compared with the signal of the predetermined frequency component constituting the original incident modulated light to detect a predetermined quantitatively measurable parameter such as a phase difference at a detection point. This parameter has a specific relationship with the absorption coefficient by an absorptive constituent in the scattering medium as being derived from the photon diffusion theory, so that the predetermined parameter is appropriately arithmetically processed to obtain various kinds of absorption information (including the linear integration value of the absorption coefficient in the scattering medium and the concentration of a specific material) associated with absorption in the scattering medium.

Claims

1. An apparatus for measuring absorption information in a scattering medium, comprising

light-emitting means for emitting modulated light having a predetermined wavelength,

light-incident means for forming the modulated light having the predetermined wavelength into a spot and causing the spot to be incident on the scattering medium,

photodetecting means for photodetecting the modulated light, changed during propagation in the scattering medium, through an opening located near an outer surface of the scattering medium,

signal extracting means for extracting a signal of one predetermined frequency component constituting the modulated light from signals photodetected by said photodetecting means,

parameter detecting means for comparing the signal extracted by the signal extracting means with the signal of the predetermined frequency component of the modulated light to be incident on the scattering medium and detecting a predetermined parameter associated with propagation of a photon density wave having the predetermined frequency component in the scattering medium and absorption of the light of the predetermined wavelength constituting the photon density wave having the predetermined frequency component,

first arithmetic processing means for calculating first absorption information associated with an absorptive constituent in the scattering medium, utilizing a relationship between the predetermined parameter and an absorption coefficient obtained when the photon density wave having the predetermined frequency component propagates in the scattering medium, and

second arithmetic processing means for calculating second absorption information associated with the absorption coefficient in the scattering medium, on the basis of a plurality of first absorption information obtained by the first arithmetic processing means.

2. An apparatus for measuring absorption information in a scattering medium according to claim 1, wherein

the modulated light emitted by the light-emitting means has at least two wavelengths that are different from each other, the absorptive constituent in the scattering medium having a different absorption coefficient with respect to each of the wavelengths of the modulated light, and

the second arithmetic processing means calculates the second absorption information with respect to a concentration of a specific absorptive constituent in the scattering medium based on the plurality of first absorption information obtained by said first arithmetic processing means for each of the wavelengths of the modulated light.

3. An apparatus for measuring absorption information in a scattering medium according to claim 1, wherein the second arithmetic processing means calculates second absorption information corresponding to a time change in a specific absorptive constituent in the scattering medium at least two different times based on the plurality of first absorption information obtained by the first arithmetic processing means.

4. An apparatus for measuring absorption information in a scattering medium according to any one of claims 1 to 3, further comprising:

scanning means for scanning the scattering medium on which the spot-like modulated light from the light-incident means is incident, and

arithmetic processing display means for generating image data by arithmetically processing position signals corresponding to scanning records of the scanning means and the second absorption information from the second arithmetic processing means, and for displaying the image data.

5. An apparatus for measuring absorption information in a scattering medium according to claim 4, wherein the predetermined parameter is a phase difference between the signal extracted by the signal extracting means and the signal having the predetermined frequency component of the modulated light to be incident on the scattering medium.

6. An apparatus for measuring absorption information in a scattering medium according to claim 5, wherein the first arithmetic processing means is further for calculating first absorption information associated with an absorptive constituent in the scattering medium based on the relationship between the predetermined parameter and the absorption coefficient, the relationship being so defined that the absorption coefficient of the absorptive constituent in the scattering medium is in inverse proportion to a square of the phase difference.

7. An apparatus for measuring absorption information in a scattering medium according to claim 4, wherein the predetermined parameter is an amplitude of the signal extracted by the signal extracting means.

8. An apparatus for measuring absorption information in a scattering medium according to any one of claims 1 to 3, further comprising displacing means for displacing an incident position of the spot-like modulated light from the light-incident means on the scattering medium along a slice of the scattering medium, and image constructing means for reconstructing a tomogram of the scattering medium based on basis of a displacement signal corresponding to a displacement by the displacing means and the second absorption information obtained by the second arithmetic processing means.

9. An apparatus for measuring absorption information in a scattering medium according to claim 8, wherein the predetermined parameter is a phase difference between the signal extracted by the signal extracting means and the signal having the predetermined frequency component of the modulated light to be incident on the scattering medium.

10. An apparatus for measuring absorption information in a scattering medium according to claim 9, wherein the first arithmetic processing means is further for calculating first absorption information associated with an absorptive constituent in the scattering medium based on the relationship between the predetermined parameter and the absorption coefficient, the relationship being so defined that the absorption coefficient of the absorptive constituent in the scattering medium is in inverse proportion to a square of the phase difference.

11. An apparatus for measuring absorption information in a scattering medium according to claim 8, wherein the predetermined parameter is an amplitude of the signal extracted by the signal extracting means.

12. An apparatus for measuring absorption information in a scattering medium according to any one of claims 1 to 3, wherein the photodetecting means comprises a plurality of photodetectors for independently detecting the modulated light components propagating through the scattering medium.

13. An apparatus for measuring absorption information in a scattering medium according to claim 12, wherein the predetermined parameter is a phase difference between the signal extracted by the signal extracting means and the signal having the predetermined frequency component of the modulated light to be incident on the scattering medium.

14. An apparatus for measuring absorption information in a scattering medium according to claim 13, wherein the first arithmetic processing means is further for calculating first absorption information associated with an absorptive constituent in the scattering medium based on the relationship between the predetermined parameter and the absorption coefficient, the relationship being so defined that the absorption coefficient of the absorptive constituent in the scattering medium is in inverse proportion to a square of the phase difference.

15. An apparatus for measuring absorption information in a scattering medium according to claim 12, wherein the predetermined parameter is an amplitude of the signal extracted by the signal extracting means.

16. An apparatus for measuring absorption information in a scattering medium according to any one of claims 1 to 3, further comprising displacing means for displacing an incident position of the spot-like modulated light from the light-incident means on the scattering medium along a slice of the scattering medium and displacing said plurality of photodetectors in synchronism with a displacement of the incident position, and image constructing means for reconstructing a tomogram of the scattering medium on the basis of a displacement signal corresponding to the displacement by the displacing means and the second absorption information obtained by the second arithmetic processing means.

17. An apparatus for measuring absorption information in a scattering medium according to claim 16, wherein the predetermined parameter is a phase difference between the signal extracted by the signal extracting means and the signal having the predetermined frequency component of the modulated light to be incident on the scattering medium.

18. An apparatus for measuring absorption information in a scattering medium according to claim 17, wherein the first arithmetic processing means is further for calculating first absorption information associated with an absorptive constituent in the scattering medium based on the relationship between the predetermined parameter and the absorption coefficient, the relationship being so defined that the absorption coefficient of the absorptive constituent in the scattering medium is in inverse proportion to a square of the phase difference.

19. An apparatus for measuring absorption information in a scattering medium according to claim 16, wherein the predetermined parameter is an amplitude of the signal extracted by the signal extracting means.

20. An apparatus for measuring absorption information in a scattering medium according to any one of claims 1 to 3, wherein the predetermined parameter is a phase difference between the signal extracted by the signal extracting means and the signal having the predetermined frequency component of the modulated light to be incident on the scattering medium.

21. An apparatus for measuring absorption information in a scattering medium according to claim 20, wherein the first arithmetic processing means is further for calculating first absorption information associated with an absorptive constituent in the scattering medium based on the relationship between the predetermined parameter and the absorption coefficient, the relationship being so defined that the absorption coefficient of the absorptive constituent in the scattering medium is in inverse proportion to a square of the phase difference.

22. An apparatus for measuring absorption information in a scattering medium according to any one of claims 1 to 3, wherein the predetermined parameter is an amplitude of the signal extracted by the signal extracting means.

23. A method of measuring absorption information in a scattering medium, comprising

the first step of emitting modulated light having a predetermined wavelength,

the second step of forming the modulated light having the predetermined wavelength light into a spot and causing the spot to be incident on the scattering medium,

the third step of photodetecting the modulated light, changed during propagation in the scattering medium, through an opening located near an outer surface of the scattering medium,

the fourth step of extracting a signal of one predetermined frequency component constituting the modulated light from signals photodetected in the third step,

the fifth step of comparing the signal extracted in the fourth step with the signal of the predetermined frequency component of the modulated light to be incident on the scattering medium and detecting a predetermined parameter associated with propagation of a photon density wave having the predetermined frequency component in the scattering medium and absorption of the light of the predetermined wavelength constituting the photon density wave having the predetermined frequency component,

the sixth step of calculating first absorption information associated with an absorptive constituent in the scattering medium, utilizing a relationship between the predetermined parameter and an absorption coefficient obtained when the photon density wave having the predetermined frequency component propagates in the scattering medium, and

the seventh step of calculating second absorption information associated with the absorption coefficient in the scattering medium, on the basis of a plurality of first absorption information obtained in the sixth step.

24. A method of measuring absorption information in a scattering medium according to claim 23, wherein

the modulated light emitted in the first step has at least two wavelengths that are different from each other, the absorptive constituent in the scattering medium having a different absorption coefficient with respect to each of the wavelengths of the modulated light, and

the second absorption information is calculated with respect to a concentration of a specific absorptive constituent in the scattering medium on the basis of the plurality of first absorption information obtained in the sixth step for each of the wavelengths of the modulated light.

25. A method of measuring absorption information in a scattering medium according to claim 23, further comprising calculating the second absorption information corresponding to a time change in a specific absorptive constituent in the scattering medium at least two different times in the seventh step based on the plurality of first absorption information obtained in the sixth step.

26. A method of measuring absorption information in a scattering medium according to any one of claims 23 to 25, wherein the predetermined parameter is a phase difference between the signal extracted by the signal extracting means and the signal having the predetermined frequency component of the modulated light to be incident on the scattering medium.

27. A method of measuring absorption information a scattering medium according to claim 26, wherein the relationship between the predetermined parameter and the absorption coefficient is so defined that the absorption coefficient of the absorptive constituent in the scattering medium is an inverse proportion to a square of the phase difference.