Method for controlling soft rot, bacterial seedling blight of rice and black rot

• US 5,441,735 A
• Filed: 06/25/1993
• Issued: 08/15/1995
• Est. Priority Date: 07/31/1992
• Status: Expired due to Term

• Current Assignee: Central Glass Company Limited
• Original Assignee: Central Glass Company Limited
• Inventors: Takahara, Yoshiyuki, Iwabuchi, Tetsuya, Shiota, Masayuki
• Primary Examiner(s): Fox, David T.
• Assistant Examiner(s): VEITENHEIMER, ERICH
• Application Number:
  US08/082,675
• Time in Patent Office: 781 Days
• Field of Search: 424/93 A, 424/93.1, 424/93.2, 424/93.4, 435/30, 435/29, 435/172.1, 435/252.1, 47/57.6, 47/DIG. 9, 47/DIG. 10
• US Class Current: 424/93.2
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 25/14 wettable
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/18 Erwinia

Abstract

A microbial pesticide containing a living Erwinia carotovora subsp. carotovora, particularly, the Erwinia cartovora subsp. carotovora CGE234M403 strain, from which the pathogenicity of soft rot is deleted by mutagenesis and which is immobilized by mixing with a saccharide such as saccharose, glucose, fructose or sorbitol or beef extract and drying or freeze-drying the mixture under reduced pressure, as an active ingredient is applied to soil or plants, which are liable to suffer from soft rot, bacterial seedling blight of rice and black rot, in the form of a suspension, granules or powder to control the diseases.

Claims

1. An Erwinia carotovora subsp. carotovora CGE234M403 strain from which the pathogenicity of soft rot is deleted by mutagenesis.

2. An agent for controlling soft rot or for controlling bacterial seedling blight of rice or for controlling black rot, which contains, as an active ingredient, Erwinia carotovora subsp. carotovora CGE234M403 strain from which the pathogenicity of soft rot is deleted by mutagenesis.

10. The agent for controlling soft rot or for controlling bacterial seedling blight of rice or for controlling black rot, according to claim 2, further comprising a chemically synthesized water-soluble high molecular weight compound, a saccharide or a protein.

11. The agent according to claim 10, in which the saccharide is selected from the group consisting of saccharose, glucose, fructose and sorbitol.

3. A method for controlling soft rot, comprising applying, to soil or a plant, Erwinia carotovora subsp. carotovora CGE234M403 strain from which the pathogenicity of soft rot is deleted by mutagenesis.

4. The method for controlling soft rot according to claim 3, in which the plant is a potato and in which the Erwinia carotovora subsp. carotovora CGE234M403 strain is applied by immersing tubers of a potato in a suspension containing said strain, and planting the tubers into soil.

5. The method for controlling soft rot according to claim 3, in which the plant is a potato and in which the Erwinia carotovora subsp. carotovora CGE234M403 strain is applied by dusting powder containing said strain over tubers of a potato, and planting the tubers into soil.

6. A method for immobilizing bacteria of soft rot, comprising mixing, with a saccharide or beef extract, Erwinia carotovora subsp. carotovora CGE234M403 from which the pathogenicity of soft rot is deleted by mutagenesis and drying or freeze-drying the resulting mixture under reduced pressure to immobilize the same.

7. The method for immobilizing bacteria of soft rot according to claim 6, wherein the saccharide is selected from the group consisting of saccharose, glucose, fructose and sorbitol.

8. A method for controlling soft rot, comprising mixing, with a saccharide or beef extract, Erwinia carotovora subsp. carotovora CGE234M403 from which the pathogenicity of soft rot is deleted by mutagenesis, drying or freeze-drying the resulting mixture under reduced pressure to immobilize the mixture, dissolving the immobilized mixture in water and applying the mixture to a plant.

9. The method for controlling soft rot according to claim 8, wherein the saccharide is selected from the group consisting of saccharose, glucose, fructose and sorbitol.

12. A method for controlling bacterial seedling blight of rice, comprising applying, to rice seeds, Erwinia carotovora subsp. carotovora CGE234M403 from which the pathogenicity of soft rot is deleted by mutagenesis.

13. The method for controlling bacterial seedling blight of rice according to claim 16, in which Erwinia carotovora subsp. carotovora CGE234M403 is applied by immersing rice seeds in a suspension containing Erwinia carotovora subsp. carotovora CGE234M403 from which the pathogenicity of soft rot is deleted by mutagenesis, and planting the rice seeds into soil.

14. The method for controlling bacterial seedling blight of rice according to claim 12, in which Erwinia carotovora subsp. carotovora CGE234M403 from which the pathogenicity of soft rot is deleted by mutagenesis is applied by dusting powder containing said Erwinia carotovora subsp. carotovora CGE234M403 over rice seeds, and planting the rice seeds into soil.

15. The method for controlling bacterial seedling blight of rice according to claim 12, in which Erwinia carotovora subsp. carotovora CGE234M403 from which the pathogenicity of soft rot is deleted by mutagenesis is applied by drenching or mixing a suspension, powder or granules containing said Erwinia carotovora subsp. carotovora CGE234M403 into nursery beds, and planting rice seeds therein.

16. The method for controlling bacterial seedling blight of rice according to claim 12, in which Erwinia carotovora subsp. carotovora CGE234M403 from which the pathogenicity of soft rot is deleted by mutagenesis is applied by spraying a suspension containing said Erwinia carotovora subsp. carotovora CGE234M403 over the soil of nursery beds in which rice seeds are planted.

17. A method for controlling black rot, comprising applying, to soil or a plant, Erwinia carotovora subsp. carotovora CGE234M403 from which the pathogenicity of soft rot is deleted by mutagenesis.