Device containing a light absorbing element for automated chemiluminescent immunoassays

• US 5,441,894 A
• Filed: 04/30/1993
• Issued: 08/15/1995
• Est. Priority Date: 04/30/1993
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Graham, Hugh W., Genger, Kevin R., Wender, David C., Coleman, Carole L., Khalil, Omar S., Hanna, Charles F.
• Primary Examiner(s): Scheiner, Toni R.
• Assistant Examiner(s): Chin, Christopher L.
• Application Number:
  US08/056,219
• Time in Patent Office: 837 Days
• Field of Search: 436/518, 436/519, 436/527, 436/528, 436/533, 436/534, 436/805, 436/809, 436/810, 436/164, 436/169, 436/172, 422/50, 422/52, 422/55, 422/60, 422/56, 422/57, 422/58, 422/102, 435/810
• US Class Current: 436/518
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

An improved device is used for performing solid-phase chemiluminescent immunoassays. The device comprises a container having a fibrous matrix and a porous absorbent material. An improvement in the device is the use of light absorbing material as the absorbent material or as a light barrier between the fibrous matrix and the absorbent material. A second improvement comprises a fibrous matrix of binderless fiber matrix, such as glass fiber. The invention improves the direct measurement of the chemiluminescent signal from a solid surface by reducing background signal. The device is disposable and is suitable for manual use or for use with an apparatus having programmed instructions. The device is designed to be employed in a variety of solid-phase diagnostic assays such as sandwich or competitive binding assays. The device is also designed for use with microparticle capture or ion capture methods of separating the immunochemical reaction products.

Claims

1. An assay performing diagnostic device comprising:

(a) a housing;

(b) a well on the housing;

(c) an opening in the well;

(d) an absorbent pad supported by the housing and located underneath the opening;

(e) a fibrous matrix supported by the housing and disposed between the opening in the well and the absorbent pad; and

(f) a non-white pigmented light absorbing material located between the fibrous matrix and absorbent pad, wherein said light absorbing material absorbs light radiated from the absorbent pad.

2. An assay performing diagnostic device as defined in claim 1, wherein said light absorbing material comprises at least one of cellulose acetate containing carbon black and polycarbonate containing Irgalan black.

3. An assay performing diagnostic device as defined in claim 1, wherein the fibrous matrix is a binderless fibrous matrix.

4. An assay performing diagnostic device as defined in claim 1, wherein the fibrous matrix is a glass fibrous matrix.

5. An assay performing diagnostic device as defined in claim 1, wherein said light absorbing material is a non-white pigment disposed within or on the absorbent pad.

6. An assay performing diagnostic device as defined in claim 5 wherein the pigment has a light absorbance greater than a light absorbance of white material.

7. An assay performing diagnostic device for use with an analytical instrument including a detector for monitoring light, the assay performing diagnostic device comprising:

(a) a housing;

(b) a well on the housing;

(c) an opening in the well;

(d) an absorbent pad supported by the housing and located underneath the opening in the well;

(e) a fibrous matrix supported by the housing and disposed between the absorbent pad and the opening in the well; and

(f) a non-white pigmented light absorbing material located between the fibrous matrix and absorbent pad, wherein said light absorbing material absorbs light radiated from the absorbent pad to reduce light radiated to the detector of the analytical instrument.

8. An assay performing diagnostic device as defined in claim 7, wherein said light absorbing material is a non-white pigment disposed within or on the absorbent pad.

9. An assay performing diagnostic device as defined in claim 8, wherein the non-white pigment is at least one of carbon black and Irgalan black.

10. An assay performing diagnostic device comprising:

(a) a housing;

(b) a well disposed on the housing;

(c) an opening in the well;

(d) a fibrous matrix supported by the housing and located underneath the opening in the well; and

(e) an absorbent pad supported by the housing and located underneath said fibrous matrix, said absorbent pad comprising a plurality of layers of absorbent material, wherein the upper most layer of absorbent material, adjacent to said fibrous matrix, contains a non-white pigment to absorb light radiated from the absorbent pad.

11. An assay performing diagnostic device for use with an analytical instrument including a detector for monitoring light, the assay performing diagnostic device comprising:

(a) a housing;

(b) a well disposed on the housing;

(c) an opening in the well;

(d) a fibrous matrix supported by the housing and located underneath the opening in the well; and

(e) an absorbent pad supported by the housing and located underneath said fibrous matrix, said absorbent pad comprising a plurality of layers of absorbent material, wherein the upper most layer of absorbent material, adjacent to said fibrous matrix, contains a non-white pigment for absorbing light radiated from the absorbent pad to reduce light radiated to the detector of the analytical instrument.