Device for automatically analyzing samples

• US 5,443,790 A
• Filed: 07/23/1992
• Issued: 08/22/1995
• Est. Priority Date: 07/26/1991
• Status: Expired due to Fees

• Current Assignee: Societe Francaise De Recherches Et D'Investissements (SFRI)
• Original Assignee: Societe Francaise De Recherches Et D'Investissements (SFRI)
• Inventors: Coeurveille, Michel, Barach, Yves, Rafilipomanana, Christiane, Millet, Magali
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): TRAN, HIEN THI
• Application Number:
  US07/917,327
• Time in Patent Office: 1,125 Days
• Field of Search: 422/63, 422/64, 422/65, 422/67, 435/292
• US Class Current: 422/63
• CPC Class Codes: G01N 2035/00346 Heating or cooling arrangements
• CPC Class Codes: G01N 2035/0484 Belt or chain
• CPC Class Codes: G01N 2035/0487 Helix or lead screw
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1004 Cleaning sample transfer devices
• CPC Class Codes: G01N 35/109 with two horizontal degrees of freedom

Abstract

Device for enabling at least one analysis to be performed automatically on various samples is provided with at least one head having at least one first subunit for sampling and distributing liquids, a working surface-for holding samples to be analyzed, reagents and reaction supports or vessels, a mechanical system for moving the at least one head within a horizontal plane above the working surface, at least one washing bowl to wash the at least one first subunit, at least one reading system to measure results of an analysis, and elements for controlling, checking and coordinating various stages of analysis. The device is particularly suitable for performing immunological tests.

Claims

1. Device for automatically analyzing samples, comprising:

at least one head comprising at least one first subunit for sampling and distributing liquids, said first subunit comprising a first vertically movable carriage having at least one first tube for distributing and aspirating liquid product, said at least one first tube being connected to an aspirating and distributing system mounted on a frame;

a working surface for fixedly holding samples to be analyzed, reagents and reaction supports;

a mechanical system for moving said at least one head within a horizontal plane above said working surface, along two perpendicular directions, said mechanical system comprising a belt integral with said at least one head and driven by motors integral with said frame;

at least one washing bowl positioned to wash said at least one first tube, said at least one washing bowl being constructed and arranged so that said at least one first tube is at least partially insertable therein, said at least one washing bowl comprising a lower orifice through which said at least one washing bowl is emptied, and said at least one washing bowl comprising at least one liquid injection nozzle for supplying washing solution to wash an outside portion of said at least one first tube when said at least one first tube is at least partially inserted in said at least one washing bowl;

means for thermally regulating reaction the supports through a thermal regulating liquid in at least partial contact with said reaction supports;

at least one reading system to measure results of reaction between samples to be analyzed and reagents; and

means for controlling, checking and coordinating said at least one head, said mechanical system, said washing bowl, said means for thermally regulating said reaction supports and said at least one reading system to automatically analyze samples.

2. The device according to claim 1, wherein said at least one head comprises at least one second subunit for washing reaction supports.

3. The device according to claim 2, wherein said at least one second subunit comprises a second carriage secured to at least one second tube for aspirating liquid contained in the reaction support, and to at least one third tube for distributing a solution in the reaction support, said at least one second tube and said at least one third tube being connected to said aspirating and distributing system.

4. The device according to claim 3, including means for affixing said at least one second tube and said at least one third tube to said second carriage for ensuring sufficient contact with a bottom of the reaction support while compensating for possible height differences between bottoms of reaction supports.

5. The device according to claim 4, wherein said means for affixing comprise a spring.

6. The device according to claim 1, wherein said at least one washing bowl further comprises at least one injection nozzle for supplying air to dry the outside portion of said at least one first tube when said at least one first tube is partially inserted in said at least one washing bowl.

7. The device according to claim 1, wherein said at least one head comprises an additional subunit including a camera for analyzing at least one of (a) proper positioning of said at least one head, (b) identification codes associated with samples, and (c) results of an analysis.

8. The device according to claim 7, wherein said camera is fixed to said at least one head and said at least one head is vertically movable.

9. The device according to claim 1, wherein said at least one reading system comprises:

a first vessel capable of containing a reference liquid;

a second vessel capable of containing a sample;

a Y-shaped optical fiber directing light towards each of said first vessel and said second vessel; and

optical means for receiving light from said first vessel and said second vessel.

10. The device according to claim 9, wherein said optical means for receiving light comprise for each of said first vessel and second vessel a photodiode or a cell associated therewith and said second vessel.

11. The device according to claim 1, wherein said means for controlling, checking and coordinating comprise data processing means capable of storing records of analysis, control identification of samples and reagents and reactions supports, control analysis stages, and assist in operation and maintenance.

12. The device according to claim 1, wherein said means for thermally regulating comprise:

a tank for containing the thermal regulating liquid;

a vat for containing the reaction supports;

a pump for circulating the thermal regulating liquid between said tank and said vat; and

means for regulating temperature of the thermal regulating liquid.