Method of producing a sealing means in a microfluidic structure and a microfluidic structure comprising such sealing means

• US 5,443,890 A
• Filed: 07/22/1993
• Issued: 08/22/1995
• Est. Priority Date: 02/08/1991
• Status: Expired due to Term

• Current Assignee: GE Healthcare Bio-Sciences AB (General Electric Company)
• Original Assignee: Pharmacia Biosensor AB
• Inventors: Ohman, Ove
• Primary Examiner(s): Loney, Donald J.
• Application Number:
  US08/090,119
• Time in Patent Office: 761 Days
• Field of Search: 428/156, 428/167, 428/172, 428/119, 428/120, 428/132, 428/137, 428/141, 428/166, 428/192, 428/213, 428/450, 264/510, 264/513, 264/167, 264/241, 264/297.1
• US Class Current: 428/167
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: Y10T 428/24562 Interlaminar spaces
• CPC Class Codes: Y10T 428/2457 Parallel ribs and/or grooves
• CPC Class Codes: Y10T 428/24612 Composite web or sheet

Abstract

A leakage-proof sealing means is provided in a microfluidic channel assembly of the type having first and second flat surface members which when pressed against each other define a microfluidic channel system between them, by recessing a sealing groove structure into the flat surface of the first member, and applying against the first member a counter mould surface having an aligned groove and/or ridge structure of smaller width than the sealing groove structure to define a mould cavity therewith, into which a fluid sealing material is introduced and hardened to form a resilient seal, the top part of which projects above the edges of the sealing groove structure while leaving sufficient space in the upper part thereof to permit complete accommodation of the top part of the resilient seal when compressed by the second assembly member.

Claims

1. A method for producing a leakage-proof sealing means in a microfluidic channel assembly comprising first and second flat surface members which when pressed against each other define at least part of a microfluidic channel system between them, which method comprises the steps of

(i) recessing at least one groove structure into a flat surface of the first flat surface member;

(ii) providing a counter mould, the mould surface of which has at least one groove structure or at least one ridge structure, said structure or structures having an extension corresponding to, but a smaller width than, the groove structure or structures of the first member;

(iii) applying the counter mould against the first member to define a mould cavity or cavities by the groove structure or structures of the first member with the counter mould portions comprising said groove or ridge structure or structures;

(iv) introducing a fluid sealing material, capable of hardening to form a resilient sealing structure, into the mould cavity or cavities; and

(v) after hardening of the fluid sealing material, removing the mould from the first member;

the top part of the mould cavity or cavities being configured in dependence of the shrinkage or expansion properties of the fluid sealing material whereby the resulting moulded resilient sealing structure projects above groove edges of the first member while leaving sufficient space in an upper part of said groove to permit complete accommodation therein of a deformed top part of the resilient sealing structure when compressed by the second member.

2. A method according to claim 1, characterized in that it further comprises the step of recessing a fluid channel groove structure into said first member.

3. A method according to claim 2, characterized in that said fluid channel groove structure is recessed simultaneously as said groove structure for the sealing material.

4. A method according to claim 1, characterized in that said fluid sealing material when hardening is capable of shrinking to an extent sufficient for providing said required space or spaces in the upper part of the sealing material groove.

5. A method according to claim 1, characterized in that the mould surface of said counter mould is elastic.

6. A microfluidic system device comprising a first member of a substantially rigid material having a substantially flat surface and designed to be applied to a substantially flat surface of a second member of a substantially rigid material, one or both of the surfaces of said members being provided with a first groove structure wherein a corresponding fluid channel system is defined between the members when applied to each other, characterized in that said first member includes at least one second groove structure having a resilient sealing means moulded into it for sealing off parts of said fluid channel system from adjacent areas, a top portion of said sealing means projecting above the surface of said first member and being spaced from at least one side edge of said second groove structure wherein the top portion is capable of being substantially accommodated in the groove space when it is deformed by the application of said two members against each other.

7. A microfluidic system device according to claim 6, characterized in that both said first and second groove structures are provided in said first member.

8. A microfluidic system device according to claim 6, characterized in that it comprises at least two separate fluid channel grooves each of which is individually enclosed by said sealing means.

9. A microfluidic system device according to claim 6, characterized in that it also comprises said second member.

10. A microfluidic system device according to claim 6, characterized in that at least said first member is made of silicon.

11. A microfluidic system device according to claim 6, characterized in that at least part of said first groove structure has a maximum height of about 50 micrometers.

12. A microfluidic system device according to claim 6, characterized in that said resilient sealing material comprises silicone rubber.

13. A microfluidic system device according to claim 6 for use in a surface plasmon resonance based optical biosensor apparatus, characterized in that said second member is a substantially flat member supporting a sensing surface.

14. A microfluidic system device according to claim 13, characterized in that at least part of said sealing means has a refractive index permitting it to serve as a temperature reference.

15. A microfluidic system device according to claim 11, wherein said first groove structure has a height of approximately 10 micrometers.