Imaging flow cytometer for imaging and analyzing particle components in a liquid sample
First Claim
1. An imaging flow cytometer for passing a sheath blow having a sheath liquid around a sample flow containing particles to be detected, irradiating a sample fine flow with light, and detecting light signals from the particles, comprising:
- an irradiation light source for illuminating particles in the sample fine flow,imaging means for imaging particles by receiving particle light images in a sample flow region of the sheath flow, andan image processor for processing the images taken by said imaging means, wherein;
said imaging means is of the split scanning type capable of repetitively scanning a band-shaped scanning region of an imaging area limited to a certain range of the imaging area within one field period, andsaid sample flow region is defined within the band-shaped scanning region of the imaging area.
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Abstract
An imaging flow cytometer for capturing images and, analyzing particle components in a liquid sample containing particle components such as blood and urine, or microparticles such as organic high molecules in a suspension. A video camera of a vertical split scanning type is used in the optical system of a conventional particle imaging type flow cytometer. Using the video camera which is capable of scanning a certain limited range in the vertical direction repeatedly in one field period, the number of particles that can be imaged in unit time may be notably increased as compared with the case of using a conventional video camera. By thus constructing, in a flow cytometer possessing a still picture imaging function, the number of captured particles can be increased.
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Citations
7 Claims
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1. An imaging flow cytometer for passing a sheath blow having a sheath liquid around a sample flow containing particles to be detected, irradiating a sample fine flow with light, and detecting light signals from the particles, comprising:
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an irradiation light source for illuminating particles in the sample fine flow, imaging means for imaging particles by receiving particle light images in a sample flow region of the sheath flow, and an image processor for processing the images taken by said imaging means, wherein; said imaging means is of the split scanning type capable of repetitively scanning a band-shaped scanning region of an imaging area limited to a certain range of the imaging area within one field period, and said sample flow region is defined within the band-shaped scanning region of the imaging area. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification