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Method for the colorimetric determination of an analyte by means of benzyl alcohol dehydrogenase and a chromogenic redox indicator

  • US 5,445,943 A
  • Filed: 04/08/1994
  • Issued: 08/29/1995
  • Est. Priority Date: 04/08/1993
  • Status: Expired due to Fees
First Claim
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1. A method for the colorimetric determination of NAD(P)H or an analyte which forms NAD(P)H, comprising the steps ofa) reacting NAD(P)H with benzyl alcohol dehydrogenase in the presence of a nitrosoaniline compound of the formula I:

  • ##STR45## wherein said nitrosoaniline compound is an electron acceptor, b) enzymatically reducing the nitrosoaniline compound to a colored quinone diimiine, andc) measuring any color produced as an indication of the amount of NAD(P)H,whereinR1 is selected from the group consisting of hydrogen, halogen, hydroxy, alkoxy, aryloxy, alkylthio or arylthio, an alkyl or alkylene which is unsubstituted or substituted by carboxy, PO3 H2, dialkylphosphinyl or SO3 H, and an amino which is unsubstituted or substituted once or twice by an alkyl which in turn can be unsubstituted or substituted by hydroxy, PO3 H2, SO3 H or carboxy, andR is a residue which produces a color by electron conjugation with a quinone diimiine system.

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