Magnetically assisted binding assays using magnetically labeled binding members
First Claim
1. An assay device for determining the presence or amount of an analyte in a test sample, said device comprises:
- (a) a reaction vessel wherein unbound and immobilized magnetically-labeled reagent are produced in relation to the amount of said analyte in said test sample;
(b) a separation means, operatively positioned with respect to said reaction vessel, for partitioning said immobilized magnetically-labeled reagent and said unbound magnetically-labeled reagent;
(c) a magnetic filed generator means, operatively positioned with respect to said reaction vessel, for the application of a magnetic field to said magnetically-labeled reagent in said reaction vessel; and
(d) a measurement means comprising a balance, operatively positioned with respect to said reaction vessel, to assess the effect of said magnetic field on said magnetically-labeled reagent as a measure of the presence or amount of said analyte in said test sample.
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Abstract
The present invention provides devices for performing binding assays. Such devices comprise (i) a reaction vessel where unbound and immobilized magnetically-labeled reagent are produced in relation to the amount of said analyte in said test sample; (ii) a separation means for partitioning said immobilized magnetically-labeled reagent and said bound magnetically-labeled reagent; (iii) a magnetic field generator means for the application of a magnetic field to said magnetically-labeled reagent; and (iv) a measurement means to assess the effect of said magnetic field on said magnetically-labeled reagent as a measure of the presence or amount of said analyte in said test sample. The device provided by the instant invention can run, for example, direct indirect, competitive, inhibition and sandwich assay formats.
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2 Claims
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1. An assay device for determining the presence or amount of an analyte in a test sample, said device comprises:
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(a) a reaction vessel wherein unbound and immobilized magnetically-labeled reagent are produced in relation to the amount of said analyte in said test sample; (b) a separation means, operatively positioned with respect to said reaction vessel, for partitioning said immobilized magnetically-labeled reagent and said unbound magnetically-labeled reagent; (c) a magnetic filed generator means, operatively positioned with respect to said reaction vessel, for the application of a magnetic field to said magnetically-labeled reagent in said reaction vessel; and (d) a measurement means comprising a balance, operatively positioned with respect to said reaction vessel, to assess the effect of said magnetic field on said magnetically-labeled reagent as a measure of the presence or amount of said analyte in said test sample. - View Dependent Claims (2)
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Specification