Raman label and its conjugate in a ligand-binding assay for a test sample analyte

• US 5,445,972 A
• Filed: 07/22/1993
• Issued: 08/29/1995
• Est. Priority Date: 10/27/1989
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Tarcha, Peter J., Rohr, Thomas E., Winter, Gary E., Cotton, Therese
• Primary Examiner(s): Saunders, David
• Assistant Examiner(s): CHIN, CHRISTOPHER L
• Application Number:
  US08/095,888
• Time in Patent Office: 768 Days
• Field of Search: 356/301, 436/501, 436/518, 436/524, 436/525, 436/537, 436/543, 436/544, 436/546, 436/56, 436/164, 436/172, 436/800, 436/547, 436/548, 435/968
• US Class Current: 436/544
• CPC Class Codes: G01N 2021/653 Coherent methods [CARS]
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 435/968 High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/801 Electron dense compounds, e.g. ferritin
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

A Raman label which, upon radiation, produces a detectable Raman scattering signal in a ligand-binding assay for an analyte in a test sample. A conjugate containing a Raman label attached to a specific binding member.

Claims

1. A conjugate comprising a Raman label capable of providing a detectable Raman scattering signal for use in a ligand-binding assay for an analyte in a test sample when said label is subjected to radiation capable of inducing a Raman scattering effect wherein said label comprises a Raman active molecule or molecules, wherein said Raman label is attached to a specific binding member.

2. The conjugate of claim 1, wherein the specific binding member is specific for the test sample analyte.

3. The conjugate of claim 1, wherein the specific binding member is specific for an ancillary specific binding member.

4. The conjugate of claim 1, wherein the specific binding member is specific for a second specific binding member and wherein the second specific binding member is specific for the test sample analyte.