Method for the purification or removal of retroviruses using sulfated cellulose
First Claim
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1. A method for the purification of a retrovirus from contaminating substances, comprising the steps of:
- (a) contacting a preparation containing a retrovirus with sulfated cellulose, said sulfated cellulose having from about 6 to 15 μ
moles sulfate per gram of cellulose, under conditions and for a time sufficient for said retrovirus to bind to said sulfated cellulose;
(b) separating the portion of the preparation which is not bound to said sulfated cellulose from said sulfated cellulose; and
(c) eluting said bound retrovirus from said sulfated cellulose, thereby recovering said retrovirus.
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Abstract
Methods and compositions useful for the separation of viruses, including retroviruses and vital vectors, from preparations are disclosed. Sulfated oligosaccharides with at least about 6 μmoles sulfate per gram of oligosaccharide are provided. In one aspect, a sulfated oligosaccharide of the present invention may be used in the purification of a virus, such as a lipid envelope virus. The present invention also discloses methods for the removal of a contaminating virus from a preparation, such as biologic therapeutics.
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Citations
5 Claims
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1. A method for the purification of a retrovirus from contaminating substances, comprising the steps of:
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(a) contacting a preparation containing a retrovirus with sulfated cellulose, said sulfated cellulose having from about 6 to 15 μ
moles sulfate per gram of cellulose, under conditions and for a time sufficient for said retrovirus to bind to said sulfated cellulose;(b) separating the portion of the preparation which is not bound to said sulfated cellulose from said sulfated cellulose; and (c) eluting said bound retrovirus from said sulfated cellulose, thereby recovering said retrovirus. - View Dependent Claims (2, 4)
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3. A method for the removal of a retrovirus from a preparation containing a retrovirus, the method comprising the steps of:
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(a) contacting the preparation with sulfated cellulose, said sulfated cellulose having about 6 to 15 μ
moles sulfate per gram of cellulose, under conditions and for a time sufficient to permit said retrovirus to bind to said sulfated cellulose; and(b) separating the portion of the preparation which is not bound to said sulfated cellulose from said sulfated cellulose. - View Dependent Claims (5)
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Specification