Method for the purification or removal of retroviruses using sulfated cellulose

• US 5,447,859 A
• Filed: 07/16/1993
• Issued: 09/05/1995
• Est. Priority Date: 07/16/1993
• Status: Expired due to Fees

• Current Assignee: Chiron Corporation (Bayer AG)
• Original Assignee: Chiron Viagene, Inc. (Bayer AG)
• Inventors: Prussak, Charles E.
• Primary Examiner(s): Knode, Marian
• Assistant Examiner(s): LANKFORD JR, LEON B
• Application Number:
  US08/093,436
• Time in Patent Office: 781 Days
• Field of Search: 435/239, 536/59, 536/33, 210/660
• US Class Current: 435/239
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2740/13022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2740/13051 Methods of production or purification of viral material
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00

Abstract

Methods and compositions useful for the separation of viruses, including retroviruses and vital vectors, from preparations are disclosed. Sulfated oligosaccharides with at least about 6 μmoles sulfate per gram of oligosaccharide are provided. In one aspect, a sulfated oligosaccharide of the present invention may be used in the purification of a virus, such as a lipid envelope virus. The present invention also discloses methods for the removal of a contaminating virus from a preparation, such as biologic therapeutics.

Claims

1. A method for the purification of a retrovirus from contaminating substances, comprising the steps of:

(a) contacting a preparation containing a retrovirus with sulfated cellulose, said sulfated cellulose having from about 6 to 15 μ

moles sulfate per gram of cellulose, under conditions and for a time sufficient for said retrovirus to bind to said sulfated cellulose;

(b) separating the portion of the preparation which is not bound to said sulfated cellulose from said sulfated cellulose; and

(c) eluting said bound retrovirus from said sulfated cellulose, thereby recovering said retrovirus.

2. The method of claim 1 wherein said sulfated cellulose has greater than 10 μ

moles sulfate per gram of cellulose.

4. The method of any one of claims 1 or 3 wherein said sulfated cellulose has about 6 μ

moles sulfate per gram of cellulose.

3. A method for the removal of a retrovirus from a preparation containing a retrovirus, the method comprising the steps of:

(a) contacting the preparation with sulfated cellulose, said sulfated cellulose having about 6 to 15 μ

moles sulfate per gram of cellulose, under conditions and for a time sufficient to permit said retrovirus to bind to said sulfated cellulose; and

(b) separating the portion of the preparation which is not bound to said sulfated cellulose from said sulfated cellulose.

5. The method of claim 3 wherein said sulfated cellulose has greater than 10 μ

moles sulfate per gram of cellulose.