Cell growth substrates

• US 5,449,383 A
• Filed: 06/01/1993
• Issued: 09/12/1995
• Est. Priority Date: 03/18/1992
• Status: Expired due to Term

• Current Assignee: Graham Johnson, Hans J. Griesser, John G. Steele, Ronald C. Chatelier
• Original Assignee: Graham Johnson, Hans J. Griesser, John G. Steele, Ronald C. Chatelier
• Inventors: Chatelier, Ronald C., Johnson, Graham, Steele, John G., Griesser, Hans J.
• Primary Examiner(s): Lilling, Herbert J.
• Application Number:
  US08/070,049
• Time in Patent Office: 833 Days
• Field of Search: 435/174, 435/180, 435/177, 435/173.4, 623/1, 623/66, 427/2.24, 427/2.25, 427/491, 427/255.6, 428/407, 428/423.5
• US Class Current: 623/1.44
• CPC Class Codes: A61F 2/06 Blood vessels
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: C08J 7/123 Treatment by wave energy or particle radiation C08J7/18 takes precedence
• CPC Class Codes: C08L 77/00 Compositions of polyamides obtained by reactions forming a carboxylic amide link in the main chain of polyhydrazides C08L79/06; of polyamideimides or polyamide acids C08L79/08; Compositions of derivatives of such polymers
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: Y10T 428/2998 including synthetic resin or polymer
• CPC Class Codes: Y10T 428/31562 Next to polyamide [nylon, etc.]

Abstract

A process for producing a polymeric surface coating which faciliates attachment of cells on a polymeric substrate. The coating is deposited by plasma deposition of an amide monomer vapour. The coated product is suitable for use in tissue culture trays and biomedical implants.

Claims

1. A polymeric material comprising:

(A) a substrate comprising a bulk polymeric material; and

(B) a thin polymeric layer which is suitable for supporting cell growth, comprising a reorientation resistant polymer comprising plasma-polymerized amide monomers presenting amide groups for the attachment of cells, wherein said amide monomers are selected from the group consisting of dimethyl formamide and amides having the formula

space="preserve" listing-type="equation">R.sup.1 --CO--N(R.sup.2)R.sup.3

wherein R¹ is an aliphatic, alicyclic, or aromatic group, each of which may be optionally substituted by halogen atoms or hydroxy groups, and R² and R³ are each independently hydrogen or an alkyl group, and wherein said thin polymer layer promotes attachment and proliferation of said cells.

3. A biomedical implant comprising the polymeric material of claim 1.

5. The polymeric material according to claim 1, wherein said material is suitable as a support for the growth of human endothelial cells.

7. The polymeric material according to claim 1, wherein said bulk polymeric material is a natural or synthetic addition condensation polymer.

8. The polymeric material according to claim 1, wherein said bulk polymeric material is selected from the group consisting of perfluorinated polymers, polyethylene, polyesters, polyurethanes, and copolymers and mixtures thereof.

9. The polymeric material according to claim 1, wherein said substrate further comprises intermediate layers between said bulk polymeric material and said thin polymer layer.

10. The polymeric material according to claim 1, wherein said reorientation resistant polymer further comprises plasma-polymerized organic amine monomers.

2. A polymeric material suitable as a support for cell growth, which has been produced by providing a substrate comprising a bulk polymerization material and depositing a surface layer on said substrate by plasma polymerization of a vapor composition containing at least one plasma polymerizable amide monomer selected from the group consisting of dimethyl formamide and amides having the formula

View Dependent Claims (4, 6)

4. A biomedical implant comprising the polymeric material of claim 2.

6. The polymeric material according to claim 2, wherein said material is suitable as a support for the growth of human endothelial cells.

11. A process for producing a biomedical implant, comprising shaping a biomedically suitable polymeric material into an implant shape under dust-free, sterile conditions, and depositing thereon a surface layer by plasma polymerization of a vapor composition containing at least one plasma polymerizable amide monomer.