Apparatus and method for culturing embryonic stem cells
First Claim
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1. An apparatus for co-culturing embryos with embryonic stem cells comprising a solid support having at least one tapering depression, said tapering depression being about 0.2-0.3 mm in diameter at the top and 0.1-0.2 mm at the bottom wherein a controlled concentration of a selected cell-type can be layered into the tapering depression.
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Abstract
Apparatus and method for culturing at least two different cell types in close proximity are provided. A solid support having at least one depression wherein a controlled concentration of a selected cell type can be layered is employed in the invention.
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Citations
5 Claims
- 1. An apparatus for co-culturing embryos with embryonic stem cells comprising a solid support having at least one tapering depression, said tapering depression being about 0.2-0.3 mm in diameter at the top and 0.1-0.2 mm at the bottom wherein a controlled concentration of a selected cell-type can be layered into the tapering depression.
Specification
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Current AssigneeThomas Jefferson University (Main Line Health System)
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Original AssigneeThomas Jefferson University (Main Line Health System)
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InventorsKhillan, Jaspal S.
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Primary Examiner(s)Beisner, William H.
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Assistant Examiner(s)LEITH, NANCY J
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Application NumberUS08/187,395Time in Patent Office595 DaysField of Search435/240.2, 435/240.23, 435/284, 435/287, 435/297, 435/285, 435/301US Class Current435/288.3CPC Class CodesC12M 21/06 for in vitro fertilizationC12M 23/02 Form or structure of the ve...C12M 35/08 Chemical, biochemical or bi...C12N 2502/02 embryonic cellsC12N 5/0604 Whole embryos; Culture medi...C12N 5/0606 Pluripotent embryonic cells...
