Methods and reagents for HLA class I A locus DNA typing

• US 5,451,512 A
• Filed: 09/28/1993
• Issued: 09/19/1995
• Est. Priority Date: 11/05/1991
• Status: Expired due to Term

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Hoffmann-La Roche Incorporated (Roche Holding AG)
• Inventors: Bugawan, Teodorica L., Erlich, Henry A., Apple, Raymond J.
• Primary Examiner(s): Parr, Margaret
• Assistant Examiner(s): CAMPBELL, EGGERTON A
• Application Number:
  US08/127,954
• Time in Patent Office: 721 Days
• Field of Search: 435/6, 435/91.2, 935/77, 935/78, 536/23.5, 536/24.33
• US Class Current: 435/91.2
• CPC Class Codes: C12Q 1/6881 for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

Primers for amplification of specific nucleic acid sequences of the second and third exon of HLA Class I A gene and probes for identifying polymorphic sequences contained in the amplified DNA can be used in processes for typing homozygous or heterozygous samples from a variety of sources and for detecting allelic variants not distinguishable by serological methods. This HLA-A DNA typing system can be used in a forward or reverse dot-blot format that is simple and rapid to perform, produces detectable signals in minutes, and can be used for tissue typing, determining individual identity, and identifying disease susceptible individuals.

Claims

1. A method for amplifying a region of the HLA-A locus containing the first and second exons, wherein said method consists of carrying out a polymerase chain reaction using oligonucleotide primers RAP1007 (SEQ ID NO:

51) and DB337 (SEQ ID NO;

     52).

2. A pair of oligonucleotide primers for amplifying a region of the HLA-A locus containing the first and second exons, wherein said pair of primers consists of oligonucleotide primers RAP1007 (SEQ ID NO:

51) and DB337 (SEQ ID NO;

     52).