Methods and reagents for HLA class I A locus DNA typing
First Claim
1. A method for amplifying a region of the HLA-A locus containing the first and second exons, wherein said method consists of carrying out a polymerase chain reaction using oligonucleotide primers RAP1007 (SEQ ID NO:
- 51) and DB337 (SEQ ID NO;
52).
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Accused Products
Abstract
Primers for amplification of specific nucleic acid sequences of the second and third exon of HLA Class I A gene and probes for identifying polymorphic sequences contained in the amplified DNA can be used in processes for typing homozygous or heterozygous samples from a variety of sources and for detecting allelic variants not distinguishable by serological methods. This HLA-A DNA typing system can be used in a forward or reverse dot-blot format that is simple and rapid to perform, produces detectable signals in minutes, and can be used for tissue typing, determining individual identity, and identifying disease susceptible individuals.
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Citations
2 Claims
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1. A method for amplifying a region of the HLA-A locus containing the first and second exons, wherein said method consists of carrying out a polymerase chain reaction using oligonucleotide primers RAP1007 (SEQ ID NO:
- 51) and DB337 (SEQ ID NO;
52).
- 51) and DB337 (SEQ ID NO;
-
2. A pair of oligonucleotide primers for amplifying a region of the HLA-A locus containing the first and second exons, wherein said pair of primers consists of oligonucleotide primers RAP1007 (SEQ ID NO:
- 51) and DB337 (SEQ ID NO;
52).
- 51) and DB337 (SEQ ID NO;
Specification