Eliminating agent for glycosylated hemoglobin and a test method using the same

• US 5,453,379 A
• Filed: 09/03/1991
• Issued: 09/26/1995
• Est. Priority Date: 11/30/1987
• Status: Expired due to Fees

• Current Assignee: Sekisui Kagaku Kogyo Kabushiki Kaisha (Sekisui)
• Original Assignee: Sekisui Kagaku Kogyo Kabushiki Kaisha (Sekisui)
• Inventors: Kawabe, Toshiki, Takechi, Masahiro, Yamazaki, Kazutoshi, Yokoi, Masayuki
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Ludlow, Jan M.
• Application Number:
  US07/758,383
• Time in Patent Office: 1,484 Days
• Field of Search: 436/15, 436/17, 436/18, 436/67, 436/161, 436/175, 530/385, 530/417
• US Class Current: 436/67
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin
• CPC Class Codes: Y10T 436/105831 Protein or peptide standard or control [e.g., hemoglobin, etc.]
• CPC Class Codes: Y10T 436/107497 Preparation composition [e.g., lysing or precipitation, etc.]
• CPC Class Codes: Y10T 436/108331 Preservative, buffer, anticoagulant or diluent
• CPC Class Codes: Y10T 436/25125 Digestion or removing interfering materials

Abstract

An eliminating agent used for the measurement of the amount of glycosylated hemoglobins in a blood sample is provided. The eliminating agent comprises condensed phosphoric acids and/or the salts thereof as the main ingredient, wherein the agent eliminates labile glycosylated hemoglobin A_1c into non-glycosylated hemoglobin and glucose. There is also provided a reagent comprising the eliminating agent and a hemolysis agent, which is used for the measurement of the amount of glycosylated hemoglobins; and an eluent comprising the eliminating agent, which is used for the separation of glycosylated hemoglobins in blood samples by ion-exchange chromatography to measure the amount of the glycosylated hemoglobins. A method for measuring the amount of glycosylated hemoglobin A_1c in a blood sample involves the use of the eliminating agent, the reagent comprising the eliminating agent and the hemolysis agent, and/or the eluent.

Claims

1. A method for measuring the amount of stable glycosylated hemoglobin A_1c or the amount of glycosylated hemoglobin components that are obtained by eliminating labile glycosylated hemoglobin A_1c component from glycosylated hemoglobin A₁ in a blood sample comprising the steps of:

admixing a blood sample containing at least erythrocytes and/or glycosylated hemoglobins with an eliminating reagent containing an eliminating agent that comprises condensed phosphoric acids and/or salts thereof, so that labile glycosylated hemoglobin A_1c, which is contained in said erythrocytes and/or present in a liberated form, is converted into non-glycosylated hemoglobin and glucose;

treating the blood sample processed in this way by ion-exchange chromatography so that non-glycosylated hemoglobins and the various types of glycosylated hemoglobins in the sample are separated from each other; and

measuring the amount of stable glycosylated hemoglobin A_1c or the amount of glycosylated hemoglobin components that are obtained by eliminating labile glycosylated hemoglobin A_1c component from glycosylated hemoglobin A₁.

2. A method according to claim 1, wherein the condensed phosphoric acids and/or salts thereof are selected from the group consisting of metaphosphoric acid, polyphosphoric acid, analogs thereof, and mixtures thereof.