Method for the non-radioactive measurement of the nucleic acid synthesis in eukaryotic cells
First Claim
1. Method for the determination of the rate of nucleic acid synthesis in a eukaryotic cell, comprising introducing a nucleotide which is non-radioactively labelled or derivatized with a hapten into said cell with the addition of a cationic liposome which does not encapsulate said nucleotide and determining the rate of nucleic acid synthesis by determining incorporation of the non-radioactively labelled or derivatized nucleotide into nucleic acids of said eukaryotic cell as a function of time.
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Abstract
For the determination of the rate of nucleic acid synthesis in eukaryotic cells a nucleotide which is non-radioactively labelled or derivatized with a hapten is introduced into the cells with the addition of liposomes and the rate of synthesis of the nucleic acids is determined by means of the incorporation of the nucleotide via its label or derivatization.
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Citations
18 Claims
- 1. Method for the determination of the rate of nucleic acid synthesis in a eukaryotic cell, comprising introducing a nucleotide which is non-radioactively labelled or derivatized with a hapten into said cell with the addition of a cationic liposome which does not encapsulate said nucleotide and determining the rate of nucleic acid synthesis by determining incorporation of the non-radioactively labelled or derivatized nucleotide into nucleic acids of said eukaryotic cell as a function of time.
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16. Method for the determination of the rate of nucleic acid synthesis in a eukaryotic cell, comprising introducing a nucleotide which is derivatized with a hapten selected from the group consisting of digoxigenin, biotin and fluorescein into said cell with the addition of a cationic liposome which does not encapsulate said nucleotide and determining the rate of nucleic acid synthesis be determining incorporation of the derivatized nucleotide into nucleic acids of said eukaryotic cell as a function of time.
- 17. Method for the determination of the rate of nucleic acid synthesis in a eukaryotic cell, comprising introducing a nucleotide which has been non-radioactively labelled with a fluorescent dye or a luminescent dye into said cell with the addition of a cationic liposome which does not encapsulate said nucleotide and determining the rate of nucleic acid synthesis by determining incorporation of the non-radioactively labelled nucleotide into nucleic acids of said eukaryotic cell as a function of time.
Specification