Holder assembly for reaction tubes

• US 5,456,360 A
• Filed: 09/30/1994
• Issued: 10/10/1995
• Est. Priority Date: 09/30/1994
• Status: Expired due to Fees

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Perkinelmer Incorporated
• Inventors: Griffin, Reginald
• Primary Examiner(s): Sewell, Paul T.
• Assistant Examiner(s): Laster, Tara L.
• Application Number:
  US08/316,299
• Time in Patent Office: 375 Days
• Field of Search: 206/503, 206/510, 206/514, 206/515, 206/518, 206/443, 206/446, 220/23.83, 220/23.86, 220/761
• US Class Current: 206/443
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders

Abstract

This invention is directed to a plastic holder assembly for loosely holding a plurality of microtiter sample tubes, which includes a tray having a plurality of holes for receiving the tubes and having opposite vertical end walls, each of which have two spaced vertically extending slots and a horizontally extending recess between the slots; a retainer releasably nestable in the tray having a corresponding plurality of holes and having opposite vertical end walls corresponding to the end walls of the tray; a U-shaped handle extending horizontally outwardly from each of the opposite retainer end walls, each handle having two legs which slide into the tray slots respectively when the retainer is nested in the tray; a tab projecting horizontally outwardly from each of the retainer end walls between each of the two legs which snap into the tray end wall recesses respectively when the retainer is nested in the tray, the retainer having an elongated slot parallel and directly adjacent each of the end walls, whereby inwardly directed finger pressure on the U-shaped handles inwardly flex the opposite ends of the retainer to release the tabs from the tray recesses respectively to facilitate removal of the retainer from the tray.

Claims

1. A plastic holder assembly for loosely holding a plurality of microtiter sample tubes comprising a tray having a plurality of holes for receiving the sample tubes, said tray having opposite vertical end walls, each of said tray end walls having two spaced vertically extending slots and a horizontally extending recess between the slots, a retainer releasably nestable in said tray having a plurality of holes corresponding to the plurality of holes in said tray for receiving the sample tubes, said retainer having opposite vertical end walls corresponding to the end walls of said tray, a U-shaped handle extending horizontally outwardly from each of said opposite retainer end walls, each of said handles having two legs which slide into said tray slots respectively when said retainer is nested in said tray, a tab projecting horizontally outwardly from each of the retainer end walls between each of said two legs which snap into said tray end wall recesses respectively when said retainer is nested in said tray, said retainer having an elongated slot parallel and directly adjacent each of said end walls, whereby inwardly directed finger pressure on said U-shaped handle inwardly flexes said opposite ends of said retainer to release said tabs from said tray recesses respectively to facilitate removal of said retainer from said tray.

2. Apparatus according to claim 1 further comprising a plastic base having a plurality of wells in a rectangular array compatible with said plurality of holes in said retainer and in said tray, said walls being dimensioned to snugly accept lower sections of said tubes, said base being nestable with said tray, said retainer and said sample tubes to form a microtiter plate assembly having a footprint of an industry standard microtiter plate assembly.

3. Apparatus according to claim 1 wherein said holder assembly is molded from reinforced polyester thermal plastic.

4. Apparatus according to claim 1 wherein the wall thickness of said holder assembly is of the order of about 1.27 mm.

5. Apparatus according to claim 1 wherein said tray and said retainer have mating beveled corners, thereby to repeatably align said retainer with said tray in the same orientation.

6. A two-piece plastic holder for loosely holding a plurality of microtiter sample tubes of a preselected design, comprising

a) a one-piece tray comprising

i) a flat, horizontal rectangular tray plate section containing a first plurality of holes in an array compatible with industry standard microtiter plate format;

ii) a first vertical tray sidewall section around said plate extending upwardly, two spaced vertically extending tray sidewall slots disposed in each of two opposite ends of said tray sidewall section, an elongated horizontal tray sidewall recess disposed between each of said two spaced tray sidewall slots;

b) a one-piece rectangular retainer releasably nestable in said tray over any sample tubes resting in said tray, comprising

i) a flat, horizontal retainer plate section containing a second plurality of holes in a rectangular array compatible with said first plurality of holes,

ii) a first vertical retainer sidewall section around said plate extending upwardly, a U-shaped handle extending horizontally outwardly from each of two opposite ends of said retainer sidewall section corresponding to said two opposite ends of said tray sidewall sections, each of said U-shaped handles having two legs which slide into said tray slots respectively when said retainer is nested in said tray, a tab projecting horizontally outwardly from said sidewall section between each of said two legs which snap into said tray sidewall recesses respectively when said retainer is nested in said tray, said retainer plate section having elongated slots parallel to and directly adjacent said opposite ends of said retainer sidewall section respectively, whereby inwardly directed finger pressure on said U-shaped handles flex said opposite ends of said retainer sidewall sections to release said tabs from said tray sidewall recesses respectively to facilitate removal of said retainer from said tray.

7. Apparatus according to claim 6 wherein said one piece tray includes a second vertical tray sidewall section around said plate extending downward from said plate.

8. Apparatus according to claim 6 wherein said one piece retainer includes a second vertical retainer sidewall section around said plate extending downwardly from said plate.

9. Apparatus according to claim 6 wherein said sample tubes have a cylindrically shaped upper section open at its top end and a closed, tapered lower section extending downwardly therefrom, each tube being of circular cross section and having a circumferential shoulder extending outwardly from said upper section at a position on said upper section spaced from the open end thereof,

said holes in said tray being slightly larger than the outside diameter of the upper section of said tubes but smaller than the outside diameter of said shoulder, said first vertical tray sidewall section having a height greater than the height of a tube resting in one of said holes, said second vertical tray sidewall section extending downwardly approximately to the bottom of the upper section of a tube resting in one of said holes;

said holes in said retainer being slightly larger than the outside diameter of the upper section of said tubes but smaller than the outside diameter of said shoulder, and

wherein when said retainer is nested in said tray, the retainer plate section lies slightly above the shoulder of a tube resting in said tray and the first tray sidewall section is about as high as said retainer sidewall section, whereby tubes resting in said tray are retained loosely both vertically and laterally.

10. Apparatus according to claim 9 wherein the holes in the tray plate section and in the retainer plate section are larger in diameter than said tubes by about 0.7 mm.

11. Apparatus according to claim 10 wherein the holes in said tray sections are counter sunk and wherein the underside of the shoulders of said tubes are correspondingly tapered.

12. Apparatus according to claim 6 wherein said first plurality and said second plurality of holes comprises up to twenty- four holes for receiving up to twenty-four microliter sample tubes in said holder.

13. Apparatus according to claim 6 wherein said tray further comprises a plurality of support ribs extending along the underside of said tray plate section between rows of holes, and wherein said retainer further comprises a plurality of support ribs extending along the upperside of said retainer plate section between rows of holes.

14. Apparatus according to claim 7 further comprising a plastic base having a plurality of wells in a rectangular array compatible with said first and said second plurality of holes, said wells being dimensioned to snugly accept lower sections of said tubes;

said base, said tray, said retainer and said sample tubes when assembled form a microtiter plate assembly having a footprint of an industry standard microtiter plate assembly.

15. Apparatus according to claim 7 wherein said first vertical tray sidewall section and said vertical retainer sidewall sections have mating beveled corners, thereby to repeatably align said retainer with said tray in the same orientation.

16. Apparatus according to claim 6 wherein said holder assembly is molded from reinforced polyester thermoplastic.

17. Apparatus according to claim 6 wherein said sections have a thickness of the order of about 1.27 mm.

18. A two-piece plastic holder for loosely holding a plurality of microtiter sample tubes of a preselected design, each having a cylindrically shaped upper section open at its top end and a closed, tapered lower section extending downwardly therefrom, each tube being of circular cross section and having a circumferential shoulder extending outwardly from said upper section at a position on said upper section spaced from the open end thereof, comprising

a) a one-piece tray comprising

i) a flat, horizontal, rectangular tray plate section containing a first plurality of holes in an array compatible with industry standard microtiter plate format, said holes being larger than the outside diameter of the upper sections of said tubes by about 0.7 mm. but smaller than the outside diameter of said shoulder, said holes being countersunk, said tray plate having a plurality of support ribs extending along the underside of the tray plate between rows of holes;

ii) a first vertical tray sidewall section around said plate extending upwardly to a height greater than the height of a tube resting in one of said holes, two-spaced vertically extending tray sidewall slots disposed in each of two opposite ends of said tray sidewall section, an elongated horizontal tray sidewall recess disposed between each of said two spaced tray sidewall slots;

iii) a second vertical tray sidewall section around said plate extending downwardly approximately to the bottom of the upper section of a tube resting in one of said holes;

b) a one-piece rectangular retainer releasably engageable inside said tray over any sample tubes resting in said tray comprising

i) a flat, horizontal rectangular plate section containing a second plurality of holes in a rectangular array compatible with said first plurality of holes, said holes being larger than the outside diameter of the upper sections of said tubes by about 0.7 mm. but smaller than the outside diameter of said shoulder, said retainer plate section having a plurality of support ribs extending along the upperside of said retainer plate section between rows of holes;

ii) a first vertical retainer sidewall section around said plate extending upwardly, a U-shaped handle extending horizontally outwardly from each of two opposite ends of said retainer sidewall section corresponding to said two opposite ends of said tray sidewall sections, each of said U-shaped handles having two legs which slide into said tray slots respectively when said retainer is nested in said tray, a tab projecting horizontally outwardly from said sidewall section between each of said two legs which snaps into said tray sidewall recesses respectively when said retainer is nested in said tray, said retainer plate section having elongated slots parallel to and directly adjacent said opposite ends of said retainer sidewall sections respectively, wherein inwardly directed finger pressure on said U-shaped handle inwardly flex said opposite ends of said retainer sidewall sections to release said tabs respectively from said tray sidewall recesses respectively to facilitate removal of said retainer from said tray;

iii) a second vertical retainer sidewall section around said retainer plate section extending downwardly from said retainer plate section; and

said sections being molded from reinforced polyester thermoplastic and said sections having a thickness of the order of about 1.27 mm, whereby when said retainer is nested in said tray, the retainer plate section lies slightly above the shoulder of a tube resting in said tray and the first tray sidewall section is about as high as said retainer sidewall section, so that the tubes resting in said tray are retained loosely both vertically and laterally.

19. Apparatus according to claim 18 further comprising a plastic base having a plurality of wells in a rectangular array compatible with said first and second plurality of holes, said wells being dimensioned to snugly accept lower sections of said tubes, said base being assembled with said tray and said retainer and said sample tubes to form a microtiter plate assembly having a footprint of an industry standard microtiter plate assembly.

20. Apparatus according to claim 18 wherein said vertical tray sidewall section and said vertical retainer sidewall section have mating beveled corners, thereby to align said retainer with said tray when assembled.