Device and process for removing free hemoglobin from blood

• US 5,456,835 A
• Filed: 11/08/1993
• Issued: 10/10/1995
• Est. Priority Date: 11/08/1993
• Status: Expired due to Fees

• Current Assignee: Pall Corporation (Danaher Corporation)
• Original Assignee: HemaSure, Inc.
• Inventors: Lee, Eric K., Azad, Abdul R. M., Castino, Franco
• Primary Examiner(s): KIM, SUN U
• Application Number:
  US08/148,883
• Time in Patent Office: 701 Days
• Field of Search: 210/490, 210/491, 210/505, 210/508, 210/645, 210/767, 428/283, 428/286, 428/287, 428/323, 428/327, 435/2, 435/179, 435/181, 436/177, 436/530, 436/15, 530/385, 530/412, 530/413, 530/814, 530/816
• US Class Current: 210/645
• CPC Class Codes: A61M 2202/0057 retained by adsorption
• CPC Class Codes: A61M 2202/0439 White blood cells; Leucocytes lymphocytes A61M2202/0407
• CPC Class Codes: B01D 39/1623 of synthetic origin
• CPC Class Codes: B01D 67/0093 Chemical modification
• CPC Class Codes: B01D 67/00931 by introduction of specific groups after membrane formation, e.g. by grafting
• CPC Class Codes: B01D 69/08 Hollow fibre membranes manufacture of hollow fibres D01D5/24, D01F1/08
• CPC Class Codes: B01D 71/68 Polysulfones; Polyethersulfones
• CPC Class Codes: B01J 20/261 obtained by reactions only involving carbon to carbon unsaturated bonds macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F
• CPC Class Codes: B01J 20/262 obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G
• CPC Class Codes: B01J 20/264 derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers
• CPC Class Codes: B01J 20/28014 characterised by their form
• CPC Class Codes: B01J 20/28023 Fibres or filaments fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence
• CPC Class Codes: B01J 20/28028 Particles immobilised within fibres or filaments
• CPC Class Codes: B01J 20/28033 Membrane, sheet, cloth, pad, lamellar or mat
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3217 Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3246 having a well defined chemical structure
• CPC Class Codes: B01J 20/3248 the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
• CPC Class Codes: B01J 20/3251 comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
• CPC Class Codes: B01J 20/3255 : comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures
• CPC Class Codes: B01J 20/3293 : Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
• CPC Class Codes: G01N 33/5002 : Partitioning blood components
• CPC Class Codes: Y10S 530/814 : Cellulose or derivatives thereof
• CPC Class Codes: Y10S 530/816 : Attached to the carrier via a bridging agent
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A filter and a hollow-fiber or flat-sheet membrane for removing hemoglobin from whole blood or blood fractions are disclosed. The filter comprises a laid textile web which has been modified to attach a ligand for hemoglobin. The membrane comprises a polyethersulfone membrane that has been similarly modified. Methods for removing hemoglobin, cellular components and debris from blood are also disclosed.

Claims

1. A filter for removing hemoglobin from whole blood or a blood fraction, comprising a shape-sustaining laid textile web having a thickness of 1 to 8 mm and a bulk density of 0.05 to 0.4 g/cm³, said web comprising:

(a) a plurality of interlocked textile fibers with average deniers between 0.05 and 0.75 and average lengths between 3 mm and 15 mm, said textile fibers being substantially uniformly distributed in said web so as to form a matrix of the textile fibers with spaces between adjacent interstices of interlocked fibers; and

(b) a plurality of fibrillated particles of surface-modified polymeric material having a surface area of 5 to 60 square meters per gram substantially disposed within said spaces of the matrix, said fibrillated particles having a plurality of fine fibrils which are interlocked with adjacent textile fibers of said spaces such that the fibrillated particles are not substantially displaceable from said web during filtration of said blood;

wherein the weight ratio of the fibrillated particles to the textile fibers is between 1;

99 and 40;

60; and

wherein said surface-modified polymeric material is cellulose acetate covalently linked to a ligand for hemoglobin; and

wherein said textile fibers are hemolytically inert and stable to alkaline hydrolysis.

2. A filter according to claim 1 wherein said ligand for hemoglobin is selected from the group consisting of inositol hexaphosphate, adenosine triphosphate (ATP), pyridoxal phosphate, 2,3-diphosphoglycerate (DPG), adenosine diphosphate (ADP), and adenosine phosphate.

3. A filter according to claim 2 wherein said ligand is ATP or DPG.

4. A filter according to claim 1 wherein said ligand is attached to said cellulose acetate via a dihydrazide or diamine linker.

5. A filter according to claim 4 wherein said linker is adipic dihydrazide.

6. A filter according to claim 1 wherein said textile fibers are one or more of polyolefin, polyamide, polysulfone, polyester, polyvinyl alcohol, and poly(ethylene-vinyl alcohol) copolymer fibers.

7. A filter according to claim 6 wherein said textile fibers are polyolefin fibers.

8. A method for removing hemoglobin from whole blood or a blood fraction comprising passing said blood through a filter according to claim 3.