Glycoform fractions of recombinant soluble complement receptor 1 (sCR1) having extended half-lives in vivo

• US 5,456,909 A
• Filed: 08/07/1992
• Issued: 10/10/1995
• Est. Priority Date: 08/07/1992
• Status: Expired due to Term

• Current Assignee: Celldex Therapeutics Incorporated
• Original Assignee: T Cell Sciences, Inc. (Celldex Therapeutics Incorporated), Smithkline Beecham PLC
• Inventors: Yeh, Chang-Jing G., Freeman, Anne M., Marsh, Henry C. Jr., Lifter, John, Gosselin, Michael L., Smith, Richard A. G.
• Primary Examiner(s): Draper, Garnette D.
• Assistant Examiner(s): Fitzgerald, David L.
• Application Number:
  US08/927,099
• Time in Patent Office: 1,159 Days
• Field of Search: 530/386, 530/395, 530/412, 514/8, 435/69.6, 424/94.63, 424/94.64
• US Class Current: 424/94.63
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/166 Streptokinase
• CPC Class Codes: A61K 38/49 Urokinase; Tissue plasminogen activator
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: A61P 37/06 Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 7/02 Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748

Abstract

The present invention relates to novel glycoforms and preparations of the soluble complement receptor type 1 (sCR1), and their uses in the therapy of complement mediated diseases and disorders involving inflammation and inappropriate complement activation and in thrombotic or shock state conditions. The invention provides novel glycoforms and methods for producing, detecting, enriching and purifying such glycoforms. The invention further provides methods of specifically producing a glycoform by recombinant or chemical means. Preferred embodiments of the invention include sialylated glycoforms and glycoforms with a pI≦5.1 determined by chromatofocusing or with a sialic acid to mannose molar ratio of >0.25. The glycoforms may be formulated alone in therapeutic compositions or in combination with thrombolytic agents.

Claims

1. A composition comprising sCR1 glycoprotein molecules, in which said composition exhibits dominant isoforms of sCR1 glycoprotein molecules which have an isoelectric point, pI, less than or equal to 5.1 as determined by chromatofocusing wherein the pI of said dominant isoforms increases after neuraminidase treatment.

2. The composition of claim 1, in which at least 40% of all of said sCR1 glycoprotein molecules in said composition exhibit an isoelectric point less than or equal to about 5.1 as determined by chromatofocusing.

3. The composition of claim 2, in which at least 70% of all of said sCR1 glycoprotein molecules in said composition exhibit an isoelectric point less than or equal to about 5.1 as determined by chromatofocusing.

7. A composition according to claim 1, 4, 5 or 6 wherein the sCR1 molecules have at least 25% of the functional complement-inhibitory activity of deglycosylated sCR1.

8. A pharmaceutical composition comprising a therapeutically effective amount of a composition according to claim 1 and a pharmaceutically acceptable carrier or excipient.

12. A pharmaceutical composition according to claim 8 further comprising a therapeutically effective amount of a thrombolytic agent.

16. A pharmaceutical composition according to claim 12, 13, 14 or 15, wherein said thrombolytic agent is selected from the group consisting of:

(a) a plasminogen activator or a mutein thereof;

(b) anisoylated plasminogen-streptokinase-activator complex;

(c) single-chain urokinase;

(d) two-chain urokinase;

(e) streptokinase;

(f) a fibrinolytically active hybrid protein which comprises one chain of a first two-chain protease linked to one chain of a second two-chain protease, at least one of said first or second protease having fibrinolytic activity, such that said hybrid protein has a catalytic site essential for fibrinolytic activity which is optionally blocked by a removable blocking group; and

(g) a reversibly blocked in vivo fibrinolytic enzyme wherein the catalytic site essential for fibrinolytic activity in said enzyme is blocked by a group which is removable by hydrolysis at a rate such that the pseudo first order rate constant for hydrolysis is in the range 10^-6 sec^-1 to 10^-1 in an isotonic aqueous solution at pH 7.4 at 37°

C.

4. A composition comprising sCR1 molecules, wherein substantially all of the sCR1 molecules in said composition contain one or more complex oligosaccharide structures, wherein at least about 40% of said oligosaccharide structures are terminated with one or more sialic acid residues per oligosaccharide structure.

5. A composition according to claim 4 wherein substantially all of the sCR1 molecules in said composition contain one or more complex oligosaccharide structures, wherein at least about 70% of said oligosaccharide structures are terminated with one or more sialic acid residues per oligosaccharide structure.

9. A pharmaceutical composition comprising a therapeutically effective amount of a composition according to claim 4 and a pharmaceutically acceptable carrier or excipient.

10. A pharmaceutical composition comprising a therapeutically effective amount of a composition according to claim 5 and a pharmaceutically acceptable carrier or excipient.

13. A pharmaceutical composition according to claim 9 further comprising a therapeutically effective amount of a thrombolytic agent.

14. A pharmaceutical composition according to claim 10 further comprising a therapeutically effective amount of a thrombolytic agent.

6. A composition comprising sCR1 molecules, wherein all of the sCR1 molecules in said composition form a population of sCR1 molecules having a molar ratio of sialic acid to mannose of greater than or equal to 0.25.

11. A pharmaceutical composition comprising a therapeutically effective amount of a composition according to claim 6 and a pharmaceutically acceptable carrier or excipient.

15. A pharmaceutical composition according to claim 11 further comprising a therapeutically effective amount of a thrombolytic agent.