High-affinity RNA ligands of basic fibroblast growth factor
First Claim
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1. A method for identifying nucleic acid ligands to basic fibroblast growth factor (bFGF) comprising:
- a) preparing a candidate mixture of nucleic acids,b) contacting the candidate mixture with bFGF, wherein nucleic acids having sequences with an increased affinity to bFGF relative to the total candidate mixture may be partitioned from the remainder of the nucleic acids in the candidate mixture,c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture, andd) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for sequences with increased affinity to bFGF, whereby nucleic acid ligands to bFGF may be identified.
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Abstract
Methods are described for the identification and preparation of nucleic acid ligands and ligand solutions to basic fibroblast growth factor (bFGF). Included in the invention are nucleic acid ligands to bFGF which are inhibitors of bFGF and 2'"'"'-amino-modified RNA ligands to bFGF.
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6 Claims
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1. A method for identifying nucleic acid ligands to basic fibroblast growth factor (bFGF) comprising:
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a) preparing a candidate mixture of nucleic acids, b) contacting the candidate mixture with bFGF, wherein nucleic acids having sequences with an increased affinity to bFGF relative to the total candidate mixture may be partitioned from the remainder of the nucleic acids in the candidate mixture, c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture, and d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for sequences with increased affinity to bFGF, whereby nucleic acid ligands to bFGF may be identified. - View Dependent Claims (2, 3, 4, 5, 6)
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