BMP-6 proteins

• US 5,459,047 A
• Filed: 05/27/1994
• Issued: 10/17/1995
• Est. Priority Date: 07/01/1986
• Status: Expired due to Term

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Wozney, John M., Wang, Elizabeth A., Rosen, Vicki A., Celeste, Anthony J.
• Primary Examiner(s): Ellis, Joan
• Application Number:
  US08/251,069
• Time in Patent Office: 508 Days
• Field of Search: 435/69.1, 435/320.1, 435/235.1, 435/252.3, 435/240.2, 530/300, 530/350, 514/2, 536/23.1
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 24/043 Mixtures of macromolecular materials
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: Y10S 530/84 Bones; tendons; teeth; cartilage

Abstract

Purified BMP-6 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.

Claims

1. A composition comprising a purified human BMP-6 protein having the amino acid sequence set forth in FIG. 3 or a biologically active fragment thereof wherein said fragment is capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay.

2. The composition of claim 2 wherein said amino acid sequence comprises amino acid #412 to amino acid #513 of FIG. 3.

3. The composition of claim 2 wherein said amino acid sequence comprises amino acid #388 to amino acid #513 of FIG. 3.

4. The composition of claim 2 wherein said amino acid sequence comprises amino acid #382 to amino acid #513 of FIG. 3.

5. The composition of claim 2 further comprising a pharmaceutically acceptable matrix.

6. The composition of claim 6 wherein said matrix comprises a material selected from the group consisting of hydroxyapatite, collagen, polylactic acid and tricalcium phosphate.

7. A composition comprising a purified human BMP-6 protein produced by the steps of

(a) culturing in a suitable culture medium a cell transformed with a DNA sequence comprising the nucleotide sequence of FIG. 3 or a fragment thereof which encodes a biologically active peptide capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay;

and

(b) recovering from said culture medium a protein having the amino acid set forth in FIG. 3 or said biologically active peptide.

8. The composition of claim 8 wherein said host cells arc mammalian.

9. The composition of claim 8 wherein said host cells are CIIO cells.

10. A composition of claim 8 further comprising a pharmaceutically acceptable matrix.

11. The composition of claim 8 wherein said matrix comprises a material from the group consisting of hydroxyapatite, collagen, polylactic acid and tricalcium phosphate.

12. The composition of claim 8 wherein said protein recovered from the culture medium comprises amino acid #412 to #513 of FIG. 3.

13. The composition of claim 8 wherein said protein recovered from the medium comprises amino acid #388 to #513 of FIG. 3.

14. The composition of claim 8 wherein said protein recovered from the medium comprises amino acid #382 to #513 of FIG. 3.