Automatic filling micropipette with dispensing means

• US 5,460,782 A
• Filed: 07/18/1994
• Issued: 10/24/1995
• Est. Priority Date: 07/18/1994
• Status: Expired due to Term

• Current Assignee: Safe-Tec Clinical Products LLC
• Original Assignee: Safe-Tec Clinical Products Inc.
• Inventors: Coleman, Charles M., Kendrick, William
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Le, Long V.
• Application Number:
  US08/276,194
• Time in Patent Office: 463 Days
• Field of Search: 422/99, 422/100, 73/864.02, 73/864.11, 73/864.16, 222/206, 222/210, 222/249, 436/174, 436/180, 436/810, 128/760, 128/765, 128/766, 128/767, 604/187, 604/34
• US Class Current: 422/520
• CPC Class Codes: B01L 3/022 Capillary pipettes, i.e. having very small bore B01L3/0224 - B01L3/0237 take precedence
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An automatic filling micropipette is formed from a tubular body having an open end, and being closed or having a piston at an opposite end. The tubular body is sized to permit liquid to flow into the tubular body by capillary action. An aperture is provided in the sidewall, the aperture having a diameter smaller than the diameter of the open end.

Claims

1. An automatic filling micropipette comprising a tubular body having a closed end, an open end having an opening of a selected diameter and a sidewall extending therebetween, the tubular body having an inner diameter sized to permit liquid to flow into the tubular body by capillary action, at least a portion of the tubular body being flexible, and the sidewall having an aperture therethrough, the aperture having a diameter smaller than the diameter of the open end and being positioned to allow air to escape from the tubular body only until a volume of liquid has entered the tubular body.

2. The automatic filling micropipette of claim 1 wherein the tubular body is comprised of:

a. a sample collection tube containing the open end and the aperture; and

b. a flexible hollow member attached to the sample collection tube in a manner so that compression of the flexible member will cause air contained within the flexible hollow member to be expelled from the flexible member into the sample collection tube.

3. The automatic filling micropipette of claim 2 wherein the sample collection tube is comprised of a wettable thermoplastic.

4. The automatic filling micropipette of claim 3 wherein the wettable thermoplastic is selected from the group of thermoplastics consisting of acrylonitrile barrier resins, polyether block polyamides, cellulose acetate propionate, and butyrate.

5. The automatic filling micropipette of claim 2 wherein the sample collection tube is comprised of one of a wettable plastic, glass, metal, and a ceramic.

6. The automatic filling micropipette of claim 2 wherein the sample collection tube is comprised of a polymer which has been treated in a manner to render the polymer wettable.

7. The automatic filling micropipette of claim 2 wherein the sample collection tube has an interior surface which has been treated to render the interior surface wettable.

8. The automatic filling micropipette of claim 2 wherein the diameter of the aperture is from 0.1 to 0.4 millimeters and the diameter of the open end is from 0.2 to 4 millimeters.

9. An automatic filling micropipette comprising

a. a sample collection tube having an open distal end of a selected diameter, an open proximal end, and a sidewall extending therebetween, the sample collection tube having an inner diameter sized to permit liquid to flow into the sample collection tube by capillary action, and the sidewall having an aperture therethrough, the aperture having a diameter smaller than the diameter of the distal end and being positioned to allow air to escape from the tubular body only until a volume of liquid has entered the tubular body;

and

b. a piston inserted into the sample collection tube through the proximal end.

10. The automatic filling micropipette of claim 9 wherein the distal end of the sample collection tube is frustro-conical.

11. The automatic filling micropipette of claim 9 wherein the piston has a tapered nose at one end which nose is within the sample collection tube.

12. The automatic filling micropipette of claim 9 wherein the sample collection tube is comprised of a wettable thermoplastic.

13. The automatic filling micropipette of claim 12 wherein the wettable thermoplastic is selected from the group of thermoplastics consisting of acrylonitrile barrier resins, polyether block polyamides, cellulose acetate propionate, and butyrate.

14. The automatic filling micropipette of claim 9 wherein the sample collection tube is comprised of one of a wettable plastic, glass, metal, and a ceramic.

15. The automatic filling micropipette of claim 9 wherein the sample collection tube is comprised of a polymer which has been treated in a manner to render the polymer wettable.

16. The automatic filling micropipette of claim 9 wherein the sample collection tube has an interior surface which has been treated to render the interior surface wettable.

17. The automatic filling micropipette of claim 9 wherein the diameter of the aperture is from 0.1 to 0.4 millimeters and the diameter of the open end is from 0.2 to 4 millimeters.

18. The automatic filling device of claim 9 also comprising calibration markings on the plunger.