Apparatus for the detection of a fluid phase boundary in a transparent measuring tube and for the automatic exact metering of an amount of liquid

• US 5,463,228 A
• Filed: 12/14/1993
• Issued: 10/31/1995
• Est. Priority Date: 12/19/1992
• Status: Expired due to Fees

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Krause, Friedemann
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US08/165,859
• Time in Patent Office: 686 Days
• Field of Search: 250/577, 340/619, 356/375
• US Class Current: 250/577
• CPC Class Codes: G01F 23/292 Light , e.g. infrared or ultraviolet
• CPC Class Codes: G01F 23/2927 for several discrete levels, e.g. with more than one light-conducting sensing element
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J

Abstract

Apparatus for the detection of a phase boundary (10) in a transparent measuring tube (9), in particular of a liquid level in a rising tube (11) (LLD, Liquid Level Detector). The rising tube (11) is illuminated by an illumination device (15) and the light passing through the rising tube (11) is received by a light reception device (17) which converts it into signals dependent on the spatial distribution of the light intensity. A data processing unit (7) is used for processing the signals from the light reception device (17) into information on the height of the liquid level (10) in the rising tube (11). In such an apparatus very accurate detection can be achieved even for weakly and differently coloured liquids in very thin capillary-shaped rising tubes by means of an illumination device (15) which is designed for the spatially steady illumination of the rising tube (11), in particular with diffuse light, the light reception device (17) comprising an optical imaging system (18) for generating an image of the rising tube (11) in an image plane (19) and a row (20) of closely spaced light-sensitive elements in the image plane.

Claims

1. An apparatus for automatic exact dosing of small amounts of liquid in a medical analysis system, said apparatus comprising:

a transparent measuring tube, said measuring tube comprising a capillary tube having an inner diameter of less than 1 mm, said measuring tube also including a liquid transfer opening at a first end thereof, said liquid transfer opening for aspiration of fluid;

a fluid phase boundary detection means for automatically detecting a fluid phase boundary in said measuring tube, wherein an electrical position signal corresponding to a position of the fluid phase boundary is generated, said fluid phase boundary detection means comprising an illumination device for providing evenly distributed light onto a detection section of the measuring tube, said illumination device including at least one light source for illuminating said measuring tube, light reception means for receiving light from said illumination device, said light reception means detecting light passing through said measuring tube from said illumination device, said light reception means including at least one light detector and an optical imaging system for generating an image of the detection section in an image plane, and a plurality of closely spaced light-sensitive elements in the image plane;

said fluid phase boundary detection means further comprising data processing means for processing signals from the at least one light detector into the electrical position signal indicating the position of the fluid phase boundary in the measuring tube;

said apparatus further comprising

electronic control means for controlling aspiration and withdrawal of the fluid from the measuring tube in precisely defined amounts based upon the electrical position signal corresponding to the position of the fluid phase boundary in the measuring tube,

wherein a position of the fluid phase boundary in the detection section of the measuring tube is detected by the data processing means from a signal curve of the signals of the at least one light detector being dependent upon a spatial distribution of the intensity of the received light.

2. An apparatus as recited in claim 1, wherein said light-sensitive elements comprise CCDs.

3. An apparatus as recited in claim 1, wherein said light-sensitive elements are spaced by a distance of less than 50 μ

m.

4. An apparatus as recited in claim 1, wherein said measuring tube comprises a capillary tube having an inner diameter of less than 0.5 mm.

5. An apparatus as recited in claim 1, wherein said illumination device further comprises a light scattering device for illuminating the detection section of the measuring tube with diffused light.

6. An apparatus as recited in claim 5, wherein said light scattering device comprises a diffusion surface positioned in an essentially parallel relationship with said measuring tube.

7. An apparatus as recited in claim 5, wherein said diffusion surface is a light diffusing lighting plate, and said at least one light source is disposed on a side of the lighting plate facing away from the measuring tube.

8. An apparatus as recited in claim 1, wherein an auxiliary fluid transfer means is coupled to a second end of the measuring tube for a precisely controlled supply and removal of an auxiliary fluid to and from the measuring tube under control of the electronic control means for removal and supply of auxiliary fluid to the measuring tube, and resulting aspiration and ejection of a liquid at the first end of the measuring tube,

wherein said auxiliary fluid comprises a gas, and wherein said measuring tube is a rising tube for containing an amount of liquid, and wherein said phase boundary is a liquid level at which an amount of liquid is separated from a gas space therein, the gas space being located above the liquid with the auxiliary fluid being supplied to and discharged from the gas space in order to eject and aspirate precisely defined amounts of liquid.

9. An apparatus as recited in claim 8, wherein said auxiliary fluid comprises a gas, and wherein said measuring tube is a tube for containing an amount of liquid, and said phase boundary is a liquid level at which an amount of liquid is separated from a gas space therein, with the auxiliary fluid being supplied to and discharged from the gas space in order to eject and aspirate precisely defined amounts of liquid.

10. An apparatus as recited in claim 1, wherein said data processing means comprises filter means for smoothing the signal curve of the signals of the at least one light detector by low-pass filtering.

11. An apparatus as recited in claim 10, wherein said data processing means further comprises differentiating means for differentiation of the signal curve of the signals of the at least one light detector.

12. An apparatus as recited in claim 11, wherein said data processing means further comprises threshold means for detection of the liquid phase boundary of the signal curve of the signals of the at least one light detector by threshold detection of the differentiated signal.

13. An apparatus as recited in claim 1, wherein said data processing means further comprises differentiating means for differentiation of the signal curve of the signals of the at least one light detector.

14. An apparatus as recited in claim 13, wherein said data processing means further comprises threshold means for detection of the liquid phase boundary of the signal curve of the signals of the at least one light detector by threshold detection of the differentiated signal.