Luminescence-optical indicator for determining the activity of alkali metal ions in a sample solution

• US 5,464,587 A
• Filed: 05/16/1994
• Issued: 11/07/1995
• Est. Priority Date: 06/09/1993
• Status: Expired due to Term

• Current Assignee: Avl Medical Instruments AG (Roche Holding AG)
• Original Assignee: Avl Medical Instruments AG (Roche Holding AG)
• Inventors: Draxler, Sonja, Leiner, Marco J., Lippitsch, Max
• Primary Examiner(s): Snay, Jeffrey R.
• Application Number:
  US08/242,974
• Time in Patent Office: 540 Days
• Field of Search: 422/82.07, 422/82.08, 422/82.06, 422/82.05, 436/74, 436/79, 436/172
• US Class Current: 422/82.07
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

A luminescence-optical indicator for determining the activity of alkali metals based on decay time includes at least three components that are chemically bound to one another, i.e., a fluorophor, a functional group with which the luminescence decay time of the fluorophor can be influenced, and a ionophor binding the alkali metal ions to be measured in a selective and reversible manner, the effect of the functional group on the decay time of the fluorophor being altered in dependence on the activity of the alkali metal ions.

Claims

1. A luminescence-optical indicator for determining the activity of alkali metal ions in a sample solution through at least indirect contact with said sample solution, wherein said luminescence-optical indicator comprises at least three components:

a fluorophor having a defined luminescence decay time, a functional group with which said luminescence decay time of said fluorophor can be influenced, and a ionophor selectively and reversibly binding said alkali metal ions to be measured such that said functional group alters said decay time of said fluorophor depending on the activity of said alkali metal ions, said fluorophor being chemically bound to said ionophor and said ionophor being chemically bound to said functional group.

2. An indicator according to claim 1, wherein an absorption maximum of said fluorophor is in the wavelength range of 400-1,200 nm.

3. An indicator according to claim 1, wherein said luminescence decay time of said fluorophor is greater than 10 ns.

4. An indicator according to claim 1, wherein said fluorophor is selected from the group consisting of 2-aminoanthracene, rubrene, decacyclene, trisruthenium-bipyridyl, and tris-ruthenium-phenantroline;

wherein said functional group is selected from the group consisting of nitro-, cyano-, amino-, methoxy-groups, viologenes, halides, and pseudohalides; and

wherein said ionophor is selected from the group consisting of calix(4)arene esters, 1,4-benzodioxin-23-crown-7-lariatether, and 2,9-dibutyl-1,10-phenanthroline.

5. An indicator according to claim 1, wherein said fluorophor is selected from the group consisting of polycyclic and heterocyclic aromatics and metal ion complexes with heterocyclic ligands, whose fluorescence decay time is influenced by said functional groups.

6. An indicator according to claim 1, for determining the activity of Na⁺ ions of a sample solution wherein said fluorophor is 2-aminoanthracene, said functional group is nitrophenylmethyl, and said ionophor is a calix(4)arene.

7. An indicator according to claim 1, for determining the activity of K⁺ ions of a sample solution, wherein said fluorophor is decacyclene, said functional group is nitrophenylmethyl, and said ionophor is a benzodioxin-23-crown-7-lariatether.

8. An indicator according to claim 1, for determining the activity of K⁺ ions of a sample solution, wherein said fluorophor is tris-ruthenium-phenanthroline, said functional group is dinitrobenzene, and said ionophor is a benzodioxin-23-crown-7-lariatether.

9. An indicator according to claim 1, for determining the activity of the Li⁺ ions of a sample solution, wherein said fluorophor is rubrene, said functional group is diethylamine, and said ionophor is dibutylphenanthroline.

10. A sensor comprising a luminescence-optical indicator for determining the activity of alkali metal ions in a sample solution through at least indirect contact with said sample solution and said luminescence-optical indicator is in optical contact with an excitation and measuring unit, wherein said luminescence-optical indicator has at least three components:

a fluorophor having a defined luminescence decay time, a functional group with which said luminescence decay time of said fluorophor can be influenced, and a ionophor selectively and reversibly binding said alkali metal ions to be measured, such that said functional group alters said decay time of said fluorophor depending on the activity of said alkali metal ions, said fluorophor being chemically bound to said ionophor and said ionophor being chemically bound to said functional group, and wherein said luminescence-optical indicator is incorporated in a hydrophilic, ion-permeable matrix on a carrier substrate transparent to excitation and measurement radiation.

11. A sensor according to claim 10, wherein said luminescence-optical indicator is physically dissolved in said hydrophilic, ion-permeable matrix.

12. A sensor according to claim 10, wherein said luminescence-optical indicator is incorporated in said hydrophilic, ion-permeable matrix by electrostatic bonding.

13. A sensor according to claim 10, wherein said luminescence-optical indicator is incorporated in said hydrophilic, ion-permeable matrix by covalent bounding.