Palladium (II) octaethylporphine alpha-isothiocyanate as a phosphorescent label for immunoassays

• US 5,464,741 A
• Filed: 10/08/1993
• Issued: 11/07/1995
• Est. Priority Date: 10/08/1993
• Status: Expired due to Term

• Current Assignee: John L. Hendrix
• Original Assignee: Henwell Incorporated
• Inventors: Hendrix, John L.
• Primary Examiner(s): Spiegel, Carol A.
• Application Number:
  US08/134,133
• Time in Patent Office: 760 Days
• Field of Search: 435/5, 435/6, 435/7, 435/7.6, 435/968, 540/145, 424/1.1, 424/85.8, 424/88, 424/89, 424/1.49, 436/546, 436/548, 436/816-818
• US Class Current: 435/5
• CPC Class Codes: C07D 487/22 in which the condensed system contains four or more hetero rings
• CPC Class Codes: G01N 33/533 with fluorescent label
• CPC Class Codes: Y10S 435/968 High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
• CPC Class Codes: Y10S 436/816 Alkaloids, amphetamines, and barbiturates
• CPC Class Codes: Y10S 436/817 Steroids or hormones
• CPC Class Codes: Y10S 436/818 Human chorionic gonadotropin

Abstract

A phosphorescence assay system. The phosphorescent label for immunoassays is palladium (II) octaethylporphine alphaisothiocyanate. The labeling agent has a Stokes shift of not less than 150 nanometers. Method for preparing the palladium (II) phosphorescent label is also shown.

Claims

1. A phosphorescent labeled compound comprising a specific binding partner conjugated to a palladium (II) ochaethylporphine alpha-isothiocyanate label, said label having a Stokes shift of not less than 150 nanometers.

2. The phosphorescent labeled compound of claim 1 wherein the specific binding partner is an antigen, an antibody, or a hapten.

3. The phosphorescent labeled compound of claim 1 wherein the specific binding partner is a viral or bacterial antigen.

4. The phosphorescent labeled compound of claim 1 wherein the specific binding partner is a protein, a drug or a hormone.

5. The phosphorescent labeled compound of claim 1 wherein the label has a Stokes shift of from 200 to 300 nanometers.

6. A method of preparing a phosphorescent labeled compound which comprises:

(a) forming a mixture of (i) a palladium (II) octaethylporphine alpha-isothiocyanate having a Stokes shift of not less than 150 nanometers and (ii) a specific binding partner; and

,

(b) incubating the mixture under conditions sufficient to covalently conjugate the specific binding partner to the palladium (II) octaethylporphine alpha-isothiocyanate through an isothiocyanate moiety on the palladium (II) octaethylporphine alpha-isothiocyanate,

thereby forming the phophorescent labeled compound.

7. The method of claim 6 wherein the palladium (II) octaethylporphine alpha-thiocyanate has a Stokes shift from 200 to 300 nanometers.