Colloidal-gold electrosensor measuring device

• US 5,468,366 A
• Filed: 09/30/1994
• Issued: 11/21/1995
• Est. Priority Date: 01/15/1992
• Status: Expired due to Term

• Current Assignee: ESA Biosciences, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Andcare Incorporated
• Inventors: Wegner, Steven, Naser, Najih, McCaffrey, Terence M., Morris, Susan E., Harpold, Michael A., Zhao, Junguo, Henkens, Robert W., Wojciechowski, Marek, O'Daly, John P.
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Bell, Bruce F.
• Application Number:
  US08/316,433
• Time in Patent Office: 417 Days
• Field of Search: 204/403, 204/412, 204/415, 204/435, 204/153.12, 204/153.1, 435/817, 435/288, 436/74, 436/77
• US Class Current: 205/789.5
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/308 at least partially made of carbon
• CPC Class Codes: G01N 27/42 Measuring deposition or liberation of materials from an electrolyte; Coulometry, i.e. measuring coulomb-equivalent of material in an electrolyte
• CPC Class Codes: G01N 33/48714 for determining substances foreign to the organism, e.g. drugs or heavy metals drugs by chemical analysis G01N33/94
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The present invention provides a new device for use in measuring lead levels in biological and environmental samples. Using square wave coulometry and colloidal gold particles impregnated on carbon electrodes, the present invention provides a rapid, reliable, portable and inexpensive means of detecting low lead levels. The colloidal gold modified electrodes have microelectrode array characteristics and produce significantly higher stripping detection signals for lead than are produced at bulk gold electrode surfaces. The method is effective in determining levels of lead down to at least 5 μg/dL in blood samples as small as 10 μL.

Claims

1. An electrode comprising a conducting substrate onto which is deposited 10-30 μ

g/cm² of colloidal gold admixed with a cationic polymer.

2. The electrode of claim 1 wherein the cationic polymer is a poly(ester-sulfonic acid).

3. The electrode of claim 1 wherein the conducting substrate is glassy carbon.

4. The electrode of claim 1 wherein the conducting substrate is carbon ink.

5. The electrode of claim 4, wherein the electrode is screen printed.

10. A lead monitoring device comprising the electrode of claim 1 or claim 6, a reference electrode, a counter electrode coupled, means of measuring current, and means for performing square wave coulometric analysis.

11. A method of determining lead ion levels, comprising the steps:

contacting a sample suspected of containing lead ion with the electrode of claim 1 or the microelectrode array of claim 6;

reductively plating metallic lead onto the electrode surface;

stripping the plated lead from the electrode surface to form lead ion to produce a current; and

relating amount of current generated to the lead ion level in the sample.

12. The method of claim 11 wherein the sample is a water sample or a urine sample.

13. The method of claim 11 wherein the sample is a blood sample.

14. The method of claim 13 wherein the blood sample is admixed with an acid prior to the contacting step.

15. The method of claim 11 wherein current is determined by square wave coulometry.

16. The method of claim 11 wherein the sample volume is between 10 and 100 μ

L.

17. The method of claim 11 wherein the reductive plating is accomplished at about -0.5 volts.

18. The method of claim 11 wherein the stripping is by square wave voltammetry in a potential range between -0.5 and +0.5 volts.

6. An electrode comprising colloidal gold dispersed in a cationic polymer matrix that is deposited onto an electrode surface to form a microelectrode array.

7. The microelectrode array of claim 6 wherein the cationic polymer is poly(ester-sulfonic acid).

8. The microelectrode array of claim 6 wherein the electrode surface is screen printed carbon ink or glassy carbon.

9. The microelectrode array of claim 6 wherein size of the colloidal gold is between about 200 and 500 angstroms in diameter.

19. A system for coulometric determination of heavy metal ions that have a stripping potential between -0.5 and +1.0 volts, comprising the electrode of claim 6, a coupled working electrode and means for adjusting deposition potential between about -0.5 and +0.5 volts.

20. A method of determining blood lead ion levels in the range of about 1 to about 60 μ

g/dL comprising;

admixing acid with a blood sample suspected of containing lead;

reductively depositing the lead onto the coupled electrode of claim 6 at a potential of about -0.5 volts; and

coulometrically determining the amount of deposited lead.

21. An electrode comprising a carbon ink substrate onto which is deposited a film consisting essentially of colloidal gold particles having a size of about 200-500 Å

admixed with a poly(ester-sulfonic acid) polymer.

22. The electrode of claim 21, wherein the electrode is screen printed.