Cuvette

• US 5,472,671 A
• Filed: 02/14/1994
• Issued: 12/05/1995
• Est. Priority Date: 04/26/1989
• Status: Expired due to Term

• Current Assignee: Jan Lilja, Sven-Erik Nilsson
• Original Assignee: Jan Lilja, Sven-Erik Nilsson
• Inventors: Nilsson, Sven-Erik, Lilja, Jan
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): TRAN, HIEN THI
• Application Number:
  US08/196,640
• Time in Patent Office: 659 Days
• Field of Search: 422/57, 422/58, 422/61, 422/72, 422/101, 422/102, 422/56, 436/164, 436/63, 436/177-178, 436/180, 436/70, 436/69, 356/246
• US Class Current: 422/554
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The present invention is a cuvette provided with at least two cavities. The first or inlet cavity takes up fluid by capillary action. The second cavity is a reception cavity which communicates with the first cavity through a first channel. The second cavity preferably is constructed and arranged to not exert a capillary action, although it could be constructed to exert a capillary action. The first channel which is in fluid communication with the first cavity and the second cavity exerts a non-capillary and non-spontaneous fluid transporting function that is operative only under an external influence, such as by application of a centrifugal force on the cuvette. The cuvette is well suited for a variety of analyses, particularly upon whole blood.

Claims

1. A cuvette comprising:

a) at least one first cavity having an inlet arranged to take up a liquid sample by capillary action;

b) at least one second cavity for receiving liquid from said at least first cavity and;

c) at least one channel in communication with said at least one first cavity and said at least one second cavity for transporting liquid from a) to b) only upon the application of centrifugal force upon the cuvette, the at least one channel having a depth that is greater than the depth of the first cavity.

2. The cuvette as set forth in claim 1 wherein the cuvette is provided in the at least one channel with a hydrophobic filter.

3. The cuvette as set forth in claim 1 wherein at least one reagent is located in at least one of the at least one first cavity or the at least one second cavity.

4. The cuvette as set forth in claim 1 wherein the at least one second cavity comprises a first section in fluid communication with a second section, the first section is adjacent to the at least one channel and the second section having a depth relatively greater than the first section which is constructed and arranged to exert a capillary action.

5. The cuvette as set forth in claim 1 wherein the at least one second cavity comprises a plurality of second cavities and the at least one channel comprises a plurality of channels, and each of the second cavities is in fluid communication with a respective channel exerting a liquid transporting function operative only upon the application of an centrifugal force upon the cuvette, each of the channels being in fluid communication with the at least one first cavity.

6. The cuvette as set forth in claim 1 wherein said at least one first cavity comprises a plurality of first cavities, said at least one second cavity is a single second cavity and said at least one channel comprises a plurality of first channels, each of the first cavities in fluid communication with a respective first channel, and each of the first channels in fluid communication with the single second cavity, the single second cavity being in fluid communication with a second channel which is in fluid communication with a third cavity.

7. The cuvette as set forth in claim 6 wherein at least one of the plurality of first cavities is provided with a seal and means for perforating the seal upon the application of centrifugal force upon the cuvette.

8. The cuvette as set forth in claim 1 wherein at least a portion of the cuvette is provided with a semi-permeable membrane.