Horseshoe crab amebocyte lysate factor G activation inhibitor
First Claim
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1. An endotoxin-specific Limulus test reagent consisting essentially of horseshoe crab amebocyte lysate containing factor G and a polyglycoside composed of a poly-(1→
- 3)-β
-D-glucoside structural portion consisting of 2 to 370 (1→
3)-β
-D-glucoside structural units of the following formula;
##STR16## which are continuously bound to one another in an amount of 50 to 500 ng per ml of the horseshoe crab amebocyte lysate and sufficient to inhibit 100% of the activation of factor G, and containing no activated factor G.
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Abstract
This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1→3)-β-D-glucoside structure portion consisting of 2 to 370 (1→3)-β-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.
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Citations
28 Claims
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1. An endotoxin-specific Limulus test reagent consisting essentially of horseshoe crab amebocyte lysate containing factor G and a polyglycoside composed of a poly-(1→
- 3)-β
-D-glucoside structural portion consisting of 2 to 370 (1→
3)-β
-D-glucoside structural units of the following formula;
##STR16## which are continuously bound to one another in an amount of 50 to 500 ng per ml of the horseshoe crab amebocyte lysate and sufficient to inhibit 100% of the activation of factor G, and containing no activated factor G. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
- 3)-β
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18. An endotoxin-specific Limulus test reagent consisting essentially of horseshoe crab amebocyte lysate containing factor G and a polyglycoside composed of a poly-(1→
- 3)-β
-D-glucoside structural portion consisting of 4 to 180 (1→
3)-β
-D-glucoside structural units of the following formula;
##STR17## which are continuously bound to one another in an amount of 50 to 500 ng per ml of horseshoe crab amebocyte lysate and sufficient to inhibit 100% of the activation of factor G, and containing no activated factor G.
- 3)-β
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19. An endotoxin-specific Limulus test reagent which consists essentially of horseshoe crab amebocyte lysate containing factor G and a polyglycoside composed of a poly-(1→
- 3)-β
-D-glucoside structural portion consisting of 4 to 180 (1→
3)-β
-D-glucoside structural units bound to one another, having factor G activation inhibiting titer of at least about 10,700,000 units/mg and having a molecular weight of about 2,080 to about 5,800 in an amount of 50 to 500 ng per ml of horseshoe crab amebocyte lysate and sufficient to inhibit 100% of the activation of factor G, and containing no activated factor G. - View Dependent Claims (23, 24, 25, 26, 27)
- 3)-β
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20. An endotoxin-specific Limulus test reagent which consists essentially of horseshoe crab amebocyte lysate consisting factor G and a polyglycoside composed of a poly-(1→
- 3)-β
-D-glucoside structural portion consisting of 4 to 180 (1→
3)-β
-D-glucoside structural units bound to one another, wherein the polyglycoside is a formic acid degradation product of curdlan, and which has factor G activation inhibiting titer of at least about 10,700,000 units/mg in an amount of 50 to 500 per ml of horseshoe crab amebocyte lysate and sufficient to inhibit 100% of the activation of factor G, and containing no activated factor G. - View Dependent Claims (28)
- 3)-β
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21. An endotoxin-specific Limulus test reagent which consists essentially of horseshoe crab amebocyte lysate containing factor G and a polyglycoside composed of a poly-(1→
- 3)-β
-D-glucoside structural portion consisting of 4 to 180 (1→
3)-β
-D-glucoside structural units bound to one another, wherein the polyglycoside is a formic acid degradation product of curdlan, and which has a molecular weight of about 2,080 to about 5,800 in an amount of 50 to 500 ng per ml of horseshoe crab amebocyte lysate and sufficient to inhibit 100% of the activation of factor G, and containing no activated factor G.
- 3)-β
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22. An endotoxin-specific Limulus test reagent which consists essentially of horseshoe crab amebocyte lysate containing factor G and a polyglycoside composed of a poly-(1→
- 3)-β
-D-glucoside structural portion consisting of 4 to 180 (1→
3)-β
-D-glucoside structural units bound to one another, which has a molecular weight of about 2,000 to 5,800, wherein the polyglycoside is a formic acid degradation product of curdlan, and which has factor G activation inhibiting titer of at least about 10,700,000 units/mg in an amount of 50 to 500 ng per ml of horseshoe crab amebocyte lysate and sufficient to inhibit 100% of the activation of factor G, and containing no activated factor G.
- 3)-β
Specification