Apparatus for annotating data on an assay medium

• US 5,476,016 A
• Filed: 10/27/1993
• Issued: 12/19/1995
• Est. Priority Date: 10/27/1993
• Status: Expired due to Fees

• Current Assignee: Board of Regents of the University of Nebraska
• Original Assignee: Board of Regents of the University of Nebraska
• Inventors: Cray, William C. Jr., Fedorka-Cray, Paula J.
• Primary Examiner(s): NOLAND, THOMAS
• Application Number:
  US08/143,919
• Time in Patent Office: 783 Days
• Field of Search: 73/863, 73/866.3, 378/162, 378/164, 435/7.95, 436/808, 422/58, 422/61, 422/119
• US Class Current: 73/863
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 30/95 Detectors specially adapted therefor; Signal analysis
• CPC Class Codes: Y10S 436/808 Automated or kit

Abstract

The present invention includes an apparatus, kit and method for annotating information on an assay medium, such as an electrophoresis gel, thin-layer chromatography plate, x-ray film, and the like, prior to recording the data on the assay medium by photography or other like means. The apparatus is composed of a planar base with grooved tracks, or of two or more side rails that are placed on a planar base adjacent the assay medium, with markers for designating information about a sample or its separated components on the assay medium.

Claims

1. An apparatus for annotating an assay medium containing a sample located thereon, the apparatus comprising:

(a) a planar base having a surface with first and second grooved tracks therein, each track having a length, depth and width;

the grooved tracks aligned perpendicular to each other in the planar base to define an assay medium receiving area thereinbetween; and

(c) at least two markers, a first marker being releasably attached in the first grooved track, and a second marker being releasably attached in the second grooved track;

the markers being positionable along the grooved tracks and including indicating means to designate information about the sample or separated components thereof in an assay medium placed on the assay medium receiving area.

2. The apparatus according to claim 1, further comprising a side rail having a length, and first and second surfaces, and means for operably and releasably retaining a marker in said second surface of the side rail;

the side rail being positioned on the surface of or adjacent to the planar base to operably receive the marker in said marker retaining means to designate information about the sample in the assay medium;

the side rail being aligned perpendicular to the first or second grooved track to further define the assay medium receiving area.

3. The apparatus according to claim 2, wherein the side rail further includes means for attaching the side rail to the planar base, said attachment means being affixed to the first surface of the side rail;

said attachment means being releasably attached in the first or second grooved track in the planar base, and positionable on the surface of the planar base along the grooved track to further define the assay medium receiving area.

4. The apparatus according to claim 3, wherein the side rail is slidable within the first or second grooved track.

5. The apparatus according to claim 3, wherein the planar base further includes a third grooved track therein being aligned perpendicular to the first or second grooved track to further define the assay medium receiving area, wherein the attachment means of the side rail is releasably attached in the third grooved track.

6. The apparatus according to claim 2, wherein the marker retaining means of the side rail is a grooved track being continuous along at least part of the length of the side rail, or a plurality of discretely positioned apertures along at least a part of the length of the side rail, or a combination thereof.

7. The apparatus according to claim 1, further comprising a plurality of markers being releasably attached in the grooved tracks in the planar base, and positionable along said grooved tracks to designate information about two or more samples or separated components thereof in the assay medium.

8. The apparatus according to claim 1, wherein the markers comprise a peg, and the grooved tracks of the planar base are configured to operably and releasably receive the peg of the marker.

9. The apparatus according to claim 8, wherein the markers are slidable within at least one of the grooved tracks.

10. The apparatus according to claim 1, wherein the planar base is transparent to visible or ultraviolet light.

11. The apparatus according to claim 1, in combination with an assay medium positioned on the surface of the planar base within the assay medium receiving area.

12. The apparatus according to claim 1, further comprising means for illuminating the apparatus, being positioned Above, under or to a side of the planar base.

13. The apparatus according to claim 1, further comprising means for recording the apparatus, being positioned above, under or to a side of the planar base.

14. The apparatus according to claim 13, wherein said means for recording is a camera, videotape, autoradiographic means, digital scanning, or photocopying device.

15. An article of manufacture for use in annotating an assay medium containing a sample thereon, comprising, in combination, packaging material and an apparatus according to claim 1 contained within said packaging material.

16. The article according to claim 15, further comprising instruction means which indicate how to assemble, use, or record the apparatus, or a combination thereof.

17. The article according to claim 16 wherein the instruction means is a label or tag attached to the packaging.

18. The article according to claim 16, wherein the instruction means is a printed package insert.

19. The article according to claim 15, further comprising means for recording, means for illuminating, or a combination thereof.

20. A method of annotating an assay medium containing a sample thereon, the sample having a location in the assay medium, the method comprising:

(a) providing a planar base having first and second grooved tracks therein, and at least two markers;

the grooved tracks each having a length, depth, and width, the grooved tracks aligned perpendicular to each other in the planar base to define an assay medium receiving area thereinbetween;

the assay medium receiving area having a surface area effective for receiving the assay medium thereon;

(b) attaching a first marker to the first grooved track, and a second marker to the second grooved track;

the markers being releasably attached to the grooved tracks;

(c) placing the assay medium containing the sample on the planar base within the assay medium receiving area; and

(d) positioning the first marker in the first grooved track and the second marker in the second grooved track to denote information about the sample in the assay medium.

21. The method according to claim 20 wherein the sample comprises a plurality of separate components in the assay medium;

and the apparatus comprises a plurality of first markers in the first grooved track and a plurality of second markers in the second grooved track;

the method further comprising adjusting the markers along the lengths of the grooved tracks to designate information about the components of the sample in the assay medium.

22. The method according to claim 21, further comprising step (e) recording the markers and the sample in the assay medium.

23. The method according to claim 22, wherein the markers and the sample in the assay medium are recorded by photographing, videotaping, autoradiographic technique, digital scanning, or photocopying.

24. The method according to claim 22, further comprising illuminating the assay medium prior to and during the recording.

25. The method according to claim 20, further comprising:

(e) positioning a side rail on the surface of or adjacent to the planar base, wherein the side rail is aligned perpendicular to the first or second grooved track to further define the assay medium receiving area;

the side rail having a first and second surface, a length, and means for operably and releasably receiving a marker on said second surface of the side rail;

the second surface of the side rail being positioned for operably receiving a marker in said marker receiving means;

(f) attaching a marker to said receiving means and positioning the marker on the side rail to designate information about the sample in the assay medium.

26. The method according to claim 25, wherein the side rail further includes means for releasably attaching the side rail to the planar base, said attachment means affixed to the first surface of the side rail;

and step (e) further comprises inserting said attachment means into the first or second grooved track in the planar base, and positioning the side rail on the surface of the planar base along the grooved track to further define the assay medium receiving area.

27. The method according to claim 26, wherein the planar base further includes a third grooved track aligned perpendicular to the first or second grooved track;

and the side rail includes attachment means operable for further inserting the side rail in the third grooved track; and

step (e) further comprises attaching said attachment means in the third grooved track.