Substance BS-3

• US 5,476,656 A
• Filed: 03/17/1994
• Issued: 12/19/1995
• Est. Priority Date: 03/18/1993
• Status: Expired due to Fees

• Current Assignee: Kureha Kagaku Kogyo Kabushiki Kaisha
• Original Assignee: Kureha Kagaku Kogyo Kabushiki Kaisha
• Inventors: Fujimoto, Satoshi, Takahashi, Eiichi, Kawaguchi, Katsumi, Kase, Toshiya, Nishiki, Mitsuhiko
• Primary Examiner(s): Lilling, Herbert J.
• Application Number:
  US08/214,251
• Time in Patent Office: 642 Days
• Field of Search: 424/116, 435/101, 435/852
• US Class Current: 424/116
• CPC Class Codes: A23D 7/0056 Spread compositions
• CPC Class Codes: A61K 2800/522 Antioxidants; Radical scavengers
• CPC Class Codes: A61K 8/73 Polysaccharides
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: C08B 37/006 Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
• CPC Class Codes: C11B 5/0021 containing oxygen
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: Y10S 435/852 Klebsiella

Abstract

Substance BS-3, a polysaccharide, produced by a microorganism belonging to the genus Klebsiella is disclosed, BS-3 having the following properties: main constituting sugar: rhamnose, galactose, glucose, and glucuronic acid; white to pale brown powder; easily soluble in water and sparingly soluble in methanol, ethyl acetate, benzene, etc.; viscosity: 1 to 8,000 cps; molecular weight: 1,000 to 10,000,000; positive in phenol-sulfuric acid reaction, carbazole-sulfuric acid reaction, Molisch'"'"'s reaction, and ninhydrin reaction. BS-3 is useful as a heat-resistance stabilizer for a fat and oil emulsion and an anti-oxidant for fats and oils in an emulsion and also as an analgesic composition.

Claims

1. Substance BS-3 produced by a microorganism belonging to the genus Klebsiella, wherein the substance BS-3 has the following physicochemical properties:

(a) Appearance;

white to pale brown powder;

(b) Solubility;

easily soluble in water;

sparingly soluble in methanol, ethyl acetate, chloroform or benzene;

hydrolyzable with a mineral acid;

(c) Viscosity;

1 to 8,000 cps (0.8% aqueous solution;

30°

C.;

shear rate;

1.96 s^-1);

(d) Main constituting sugar;

rhamnose;

10.6-25.7%, galactose;

16.8-31.0%,

glucose;

25.8-36.6%, glucuronic acid;

19.8-29.7%;

(e) Color reaction;

phenol-sulfuric acid reaction;

positive,

carbazole-sulfuric acid reaction;

positive,

Molisch'"'"'s reaction;

positive,

ninhydrin reaction;

positive;

(f) Molecular weight;

1,000 to 10,000,000;

(g) Elemental analysis;

C;

30.0-40.0%, H;

4.0-6.0%, N;

0.0-1.0%;

(h) IR spectrum (KBr tablet);

3. The substance BS-3 as claimed in claim 1, wherein the substance BS-3 is a purified substance BS-3 having the following physicochemical properties:

(a) Appearance;

odorless white powder;

(b) Solubility;

easily soluble in water;

sparingly soluble in methanol, ethyl acetate, chloroform or benzene;

hydrolyzable with a mineral acid;

(c) Viscosity;

1 to 10,000 cps (0.8% aqueous solution;

30°

C.;

shear rate;

1.96 s^-1);

(d) Main constituting sugar;

rhamnose;

10.6-25.7%, galactose;

16.8-31.0%,

glucose;

25.8-36.6%, glucuronic acid;

19.8-29.7%;

(e) Color reaction;

phenol-sulfuric acid reaction;

positive,

carbazole-sulfuric acid reaction;

positive,

Molisch'"'"'s reaction;

positive,

ninhydrin reaction;

negative;

(f) Molecular weight;

10,000 to 10,000,000;

(g) Elemental analysis;

C;

31.0-40.0%, H;

4.5-6.0%, N;

0.0-1.0%;

(h) IR spectrum (KBr tablet);

5. The substance BS-3 as claimed in claim 1, wherein the microorganism is a strain Klebsiella terrigena JCM1687 (ATCC Deposit No. 55553) or a mutant thereof.

6. A process for producing the substance BS-3 as claimed in claim 1, comprising culturing a BS-3-producing microorganism belonging to the genus Klebsiella and recovering the substance BS-3 from the culture.

7. A pharmaceutical composition comprising an active ingredient together with a pharmaceutically acceptable carrier, wherein the active ingredient is the substance BS-3 of claim 1 having a molecular weight of from 10,000 to 10,000,000 which is produced by a microorganism belonging to the genus Klebsiella.

8. The pharmaceutical composition as claimed in claim 5, wherein the microorganism is a strain Klebsiella terrigena JCM1687 (ATCC Deposit No. 55553) or a mutant thereof.

2. 2930, 1610, 1410, 1310, 1160, 1070, 1040, 930, 890, 790, 600.

4. 2930, 2120, 1610, 1410, 1310, 1250, 1160, 1070, 1040, 930, 890, 840, 790, 600.

9. A pharmaceutical composition comprising an active ingredient together with a pharmaceutically acceptable carrier, wherein the active ingredient is a polysaccharide produced by a microorganism belonging to the genus Klebsiella having the following physiochemical properties:

(a) Appearance;

odorless white powder;

(b) Solubility;

easily soluble in water;

sparingly soluble in methanol, ethyl acetate, chloroform or benzene;

hydrolyzable with a mineral acid;

(c) Viscosity;

1 to 10,000 cps (0.8% aqueous solution;

30°

C.;

shear rate;

1.96 s^-1);

(d) Main constituting sugar;

rhamnose;

10.6-25.7%, galactose;

16.8-31.0%, glucose;

25.8-36.6%, glucuronic acid;

19.8-29.7%;

(e) Color reaction;

phenol-sulfuric acid reaction;

positive, carbazole-sulfuric acid reaction;

positive, Molisch'"'"'s reaction;

positive, ninhydrin reaction;

negative;

(f) Molecular weight;

10. 000 to 10,000,000;

(g) Elemental analysis;

C;

31.0-40.0%, H;

4.5-6.0%, N;

0.0-1.0%;

(h) IR spectrum (KBr tablet);

3400, 2930, 2120, 1610, 1410, 1310, 1250, 1160, 1070, 1040, 930, 890, 840, 790, 600.