Immobilized enzymes for use in an electrochemical sensor
First Claim
1. A method for preparing a composition for use in an electrochemical sensor comprising:
- a) covalently bonding a quantity of an enzyme to fine particles in powder form to immobilize said enzyme on the particles;
b) cross-linking an additional quantity of said enzyme to a non-enzyme protein with a cross-linking agent; and
c) adding the particles containing said immobilized enzyme from step a) to the enzyme cross-linked to a protein from step b) to obtain said composition.
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Abstract
A reusable, miniature, implantable electrochemical sensor, a method of making the same, and a powder therefor are provided. Enzyme material is immobilized on bulk particulate matter, and a reaction chamber of the sensor is then filled therewith. The sensor is implanted in an environment where it comes into contact with a specific component of a fluid with which the enzyme material chemically reacts to produce electrical signals for measuring the reaction. The method preferred for preparing the powder which is used in the electrochemical sensor involves first covalently bonding a quantity of an enzyme to fine particles in powder form to immobilize the enzyme is cross-linked to a non-enzyme protein with a cross-linking agent. Finally, the particles are added containing the immobilized enzyme cross-linked to a protein from the first step to the enzyme cross-linked to a protein from the second step to obtain the powder.
361 Citations
10 Claims
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1. A method for preparing a composition for use in an electrochemical sensor comprising:
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a) covalently bonding a quantity of an enzyme to fine particles in powder form to immobilize said enzyme on the particles; b) cross-linking an additional quantity of said enzyme to a non-enzyme protein with a cross-linking agent; and c) adding the particles containing said immobilized enzyme from step a) to the enzyme cross-linked to a protein from step b) to obtain said composition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification