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Immobilized enzymes for use in an electrochemical sensor

  • US 5,476,776 A
  • Filed: 07/15/1994
  • Issued: 12/19/1995
  • Est. Priority Date: 07/19/1989
  • Status: Expired due to Fees
First Claim
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1. A method for preparing a composition for use in an electrochemical sensor comprising:

  • a) covalently bonding a quantity of an enzyme to fine particles in powder form to immobilize said enzyme on the particles;

    b) cross-linking an additional quantity of said enzyme to a non-enzyme protein with a cross-linking agent; and

    c) adding the particles containing said immobilized enzyme from step a) to the enzyme cross-linked to a protein from step b) to obtain said composition.

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