Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups

• US 5,476,925 A
• Filed: 01/23/1995
• Issued: 12/19/1995
• Est. Priority Date: 02/01/1993
• Status: Expired due to Term

• Current Assignee: Northwestern University
• Original Assignee: Northwestern University
• Inventors: Gryaznov, Sergei M., Letsinger, Robert L.
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): Crane, L. Eric
• Application Number:
  US08/376,291
• Time in Patent Office: 330 Days
• Field of Search: 536/23.1, 536/24.3, 536/24.5, 514/44
• US Class Current: 536/23.1
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

Novel oligonucleotides, method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'"'"'-phosphorylated oligonucleotides.

Claims

1. An oligodeoxyribonucleotide consisting of nucleosides wherein the base is selected from adenine, thymine, cytosine, guanine and uracil and from 1 to 4 internucleosides having the structure ##STR12## and ##STR13## and wherein the remainder of the internucleoside links are phosphodiester links.

2. An oligodeoxyribonucleotide of claim 1 including 10 to 30 nucleosides.

3. An oligonucleotide of claim 1 having 1 or 2 internucleoside links of the structure:

##STR14##

4. An oligonucleotide of claim 3 having from 10 to 12 nucleosides.