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Modified nucleotides and polynucleotides and complexes form therefrom

DC CAFC
  • US 5,476,928 A
  • Filed: 02/26/1992
  • Issued: 12/19/1995
  • Est. Priority Date: 04/17/1981
  • Status: Expired due to Term
First Claim
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1. A compound useful as a probe for detecting the presence or absence of a nucleic acid, said compound having the structure:

  • ##STR26## wherein B represents a purine, 7-deazapurine, or pyrimidine moiety suitable for incorporation into a polynucleotide and covalently bonded to the C1 '"'"'-position of the sugar moiety, provided that when B is a purine or 7-deazapurine, the sugar moiety is attached at the N9 -position of the purine or deazapurine, and when B is pyrimidine, the sugar moiety is attached at the N1 position of the pyrimidine;

    wherein A represents at least three carbon atoms and an indicator molecule selected from the group consisting of fluorescent dyes, electron-dense reagents, enzymes which can be reacted with a substrate to produce a visually detectable reaction product, and radioisotopes;

    wherein B and A are covalently attached directly or through a linkage group, said linkage group not interfering substantially with detection of A;

    wherein if B is a purine, A is attached to the 8-position of the purine, if B is a 7-deazapurine, A is attached to the 7-position of the deazapurine, and if B is a pyrimidine, A is attached to the 5-position of the pyrimidine; and

    wherein each of x, y and z represents;

    ##STR27##

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