Apparatus for measuring optical information in scattering medium and method therefor

• US 5,477,051 A
• Filed: 10/05/1993
• Issued: 12/19/1995
• Est. Priority Date: 10/06/1992
• Status: Expired due to Fees

• Current Assignee: Hamamatsu Photonics KK
• Original Assignee: Hamamatsu Photonics KK
• Inventors: Tsuchiya, Yutaka
• Primary Examiner(s): Porta, David P.
• Assistant Examiner(s): Bruce, David Vernon
• Application Number:
  US08/131,678
• Time in Patent Office: 805 Days
• Field of Search: 250/339.12, 250/341.1, 250/343, 364/413.01, 364/413.02, 364/413.07, 364/413.08, 364/413.09, 364/413.1, 364/413.11, 364/413, 328/86, 328/88, 356/432, 356/441, 356/442
• US Class Current: 250/341.1
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B

Abstract

Modulated light from a light source is incident on the surface of a scattering medium, and light passing through this scattering medium is externally detected. Components having a plurality of angular frequencies ω₁ and ω₂ corresponding to photon density waves propagating in the scattering medium are extracted from this detection signal. The extracted signals corresponding to these waves are compared with a signal of original modulated light to be incident on the scattering medium to detect a quantitatively measurable predetermined parameter such as a phase difference at a detection point for each angular frequency. The detected predetermined parameters have a predetermined relationship with absorptive and scattering constituents of the scattering medium. A pair of predetermined parameters are appropriately arithmetically calculated to eliminate the scattering coefficient, so that only the absorption coefficient can be independently calculated. Various pieces of information (including the linear integration value of the absorption coefficient in the scattering medium, the concentration of the specific material in the scattering medium, and the like) associated with absorption and the like in the scattering medium can be obtained in accordance with the calculated absorption coefficient.

Claims

1. An apparatus for measuring optical information in a scattering medium containing an absorptive constituent, comprising:

light-emitting means for emitting modulated light having a single predetermined light wavelength and at least two predetermined modulation frequency components;

light-incident means for causing said modulated light to be incident on the scattering medium;

photodetecting means for photodetecting the modulated light which has been changed during propagation in the scattering medium, through an aperture located near an outer surface of the scattering medium;

signal extracting means for extracting signals of said predetermined modulation frequency components constituting the modulated light from signals photodetected by said photodetecting means;

parameter detecting means for comparing said signals extracted by said signal extracting means with reference signals of said predetermined modulation frequency components of the modulated light to be incident on the scattering medium, respectively, and detecting predetermined parameters respectively corresponding to said signals having the predetermined modulation frequency components, said parameters being associated with propagation of said modulated light in the scattering medium and scattering and absorption of said modulated light in the scattering medium; and

arithmetic processing means for calculating an absorption coefficient of the scattering medium by use of said parameters detected by the parameter detecting means, in accordance with a given relationship between the predetermined parameters and the scattering and absorption for said modulated light during propagation in the scattering medium.

2. An apparatus for measuring optical information in a scattering medium according to claim 1, wherein said arithmetic processing means further comprises means for arithmetically calculating an equivalent scattering coefficient of the scattering medium by use of said absorption coefficient.

3. An apparatus for measuring optical information in a scattering medium according to claim 1, wherein said predetermined parameter includes a phase difference (Φ

); and

said given relationship is represented by the following formula;

##EQU3## wherein Φ

is the phase difference, μ

_a is an absorption coefficient, g is an average value of cosθ

with respect to a scattering angle Θ

, μ

_s is a scattering coefficient, r is a distance between a modulated light incident point and a photodetection point, v is a speed of light in the scattering medium, and ω

is the modulation angular frequency of the modulated light.

4. An apparatus for measuring optical information in a scattering medium according to claim 1, said predetermined parameter includes an amplitude (I_p);

and

said given relationship is represented by the following formula;

##EQU4## wherein S is a number of incident photons generated by a light source, v is a speed of light in the scattering medium, M is a degree of modulation of the modulated light, α

is a photon diffusion constant, r is a distance between a modulated light incident point and a photodetection point, I_p is the amplitude, μ

_a is an absorption coefficient, g is an average value of cosθ

with respect to a scattering angle Θ

, μ

_s is a scattering coefficient, and ω

is the modulation angular frequency of the modulated light.

5. An apparatus for measuring optical information in a scattering medium according to claim 1, further comprising scanning means for scanning the scattering medium with the modulated light incident from said light-incident means, and

arithmetic display means for arithmetically processing a position signal corresponding to scanning by said scanning means and the optical information obtained by said arithmetic processing means and visually displaying the processed position signal.

6. An apparatus for measuring optical information in a scattering medium according to claim 1, further comprising displacing means for displacing an incident position of the modulated light from said light-incident means on the scattering medium along a slice of the scattering medium, and

image reconstructing means for reconstructing a tomogram of the scattering medium on the basis of a displacement signal corresponding to a displacement by said displacing means and the optical information obtained by said arithmetic processing means.

7. An apparatus for measuring optical information in a scattering medium according to claim 1, wherein said photodetecting means comprises a plurality of photodetectors for independently detecting the modulated light components propagating through the scattering medium.

8. An apparatus for measuring optical information in a scattering medium according to claim 1, wherein the predetermined parameter includes a phase difference.

9. An apparatus for measuring optical information in a scattering medium according to claim 1, wherein the predetermined parameter includes an amplitude.

10. An apparatus for measuring optical information in a scattering medium according to claim 1, wherein said arithmetic processing means further comprises means for arithmetically calculating a concentration of the absorptive constituent in the scattering medium by use of said absorption coefficient.

11. An apparatus for measuring optical information in a scattering medium according to claim 1, wherein the scattering medium contains at least two absorptive constituents;

the light-emitting means emits at least two modulated lights each of which has a single predetermined light wavelength and at least two predetermined modulation frequency components, said light wavelengths of the modulated lights being different from each other in absorption coefficients with respect to the absorptive constituents in the scattering medium;

the light-incident means causes each of said modulated lights to be incident on the scattering medium;

the photodetecting means photodetects each of the modulated lights which has been changed during propagation in the scattering medium;

the signal extracting means extracts signals of said predetermined modulation frequency components constituting each of the modulated lights;

the parameter detecting means compares said signals with reference signals of said predetermined modulation frequency components of each of the modulated lights to be incident on the scattering medium, respectively, and detects predetermined parameters respectively corresponding to said signals; and

the arithmetic processing means calculates an absorption coefficient of the scattering medium for each of said modulated lights by use of said parameters detected by the parameter detecting means, in accordance with a given relationship between the predetermined parameters and the scattering and absorption for each of said modulated lights during propagation in the scattering medium, and calculates a concentration of each of the absorptive constituents in the scattering medium by use of said absorption coefficients.

12. A method of measuring optical information in a scattering medium containing an absorptive constituent, comprising:

emitting modulated light having a single predetermined light wavelength and at least two predetermined modulation frequency components;

causing said modulated light to be incident on the scattering medium;

photodetecting the modulated light which has been changed during the propagation in the scattering medium, through an aperture located near an outer surface of the scattering medium;

extracting signals of said predetermined modulation frequency components constituting the modulated light from signals photodetected in the photodetecting step;

comparing said signals extracted in the extracting step with reference signals of said predetermined modulation frequency components of the modulated light to be incident on the scattering medium, respectively, and detecting predetermined parameters respectively corresponding to said signals having the predetermined modulation frequency components, said parameters being associated with propagation of said modulated light in the scattering medium and scattering and absorption of said modulated light in the scattering medium; and

calculating an absorption coefficient of the scattering medium by use of said parameters detected in the comparing step, in accordance with a given relationship between the predetermined parameters and the scattering and absorption for said modulated light during propagation in the scattering medium.

13. A method of measuring optical information in a scattering medium according to claim 12, further comprising calculating an equivalent scattering coefficient of the scattering medium by use of said absorption coefficient obtained in the step of calculating an absorption coefficient.

14. A method of measuring optical information in a scattering medium according to claim 12, wherein the predetermined parameter obtained in the comparing step includes a phase difference.

15. A method of measuring optical information in a scattering medium according to claim 12, further comprising a step of calculating a concentration of the absorptive constituent in the scattering medium by use of said absorption coefficient obtained in the sixth step.

16. A method of measuring optical information in a scattering medium according to claim 12, wherein the scattering medium contains at least two absorptive constituents;

in the emitting step, at least two modulated lights each of which has single predetermined light wavelength and at least two predetermined modulation frequency components are emitted, said light wavelengths of the modulated lights being different from each other in absorption coefficients with respect to the absorptive constituents in the scattering medium;

in the causing step, each of said modulated lights is entered on the scattering medium;

in the photodetecting step, each of the modulated lights which have been changed during propagation in the scattering medium is photodetected;

in the extracting step, signals of said predetermined modulation frequency components constituting each of the modulated lights are extracted;

in the comparing step, said signals are compared with reference signals of said predetermined modulation frequency components of each of the modulated lights to be incident on the scattering medium, respectively, and predetermined parameters respectively corresponding to said signals are detected; and

in the calculating step, an absorption coefficient of the scattering medium for each of said modulated lights is calculated by use of said parameters detected in the fifth step, in accordance with a given relationship between the predetermined parameters and the scattering and absorption for each of said modulated lights during propagation in the scattering medium, and a concentration of each of the absorptive constituents in the scattering medium is calculated by use of said absorption coefficients.

17. A method of measuring optical information in a scattering medium according to claim 12, wherein the predetermined parameter includes a phase difference.

18. A method of measuring optical information in a scattering medium according to claim 12, wherein said predetermined parameter includes a phase difference (Φ

); and

said given relationship is represented by the following formula;

##EQU5## wherein Φ

is the phase difference, μ

_a is an absorption coefficient, g is an average value of cosΘ

with respect to a scattering angle Θ

, μ

_s is a scattering coefficient, r is a distance between a modulated light incident point and a photodetection point, v is a speed of light in the scattering medium, and ω

is the modulation angular frequency of the modulated light.

19. A method of measuring optical information in a scattering medium according to claim 12, wherein said predetermined parameter includes an amplitude (I_p);

and

said given relationship is represented by the following formula;

##EQU6## wherein S is a number of incident photons generated by a light source, v is a speed of light in the scattering medium, M is a degree of modulation of the modulated light, α

is a photon diffusion constant, r is a distance between a modulated light incident point and a photodetection point, I_p is the amplitude, μ

_a is an absorption coefficient, g is an average value of cosΘ

with respect to a scattering angle Θ

, μ

_s is a scattering coefficient, and ω

is the modulation angular frequency of the modulated light.