Chemical sensor for detecting binding reactions

• US 5,478,756 A
• Filed: 02/08/1995
• Issued: 12/26/1995
• Est. Priority Date: 07/24/1990
• Status: Expired due to Fees

• Current Assignee: GEC Marconi Limited (Gooding Consumer Electronics Ltd.), Fisons PLC (Sanofi )
• Original Assignee: GEC Marconi Limited (Gooding Consumer Electronics Ltd.), Fisons PLC (Sanofi )
• Inventors: Gizeli, Electra, Stevenson, Adrian C.
• Primary Examiner(s): Saunders, David
• Assistant Examiner(s): Chin, Christopher L.
• Application Number:
  US08/385,737
• Time in Patent Office: 321 Days
• Field of Search: 73/587, 73/590, 73/618, 73/620, 73/627, 73/645, 73/649, 310/311, 310/312, 310/313 R, 310/313 A, 310/313 B, 310/313 D, 310/315-319, 310/327, 310/334, 310/340, 310/345, 310/363-367, 422/82.01, 436/518, 436/524, 436/527, 436/149, 436/151, 436/806
• US Class Current: 436/527
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 2291/0422 Shear waves, transverse waves, horizontally polarised waves
• CPC Class Codes: G01N 2291/0423 Surface waves, e.g. Rayleigh waves, Love waves
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 29/2462 Probes with waveguides, e.g. SAW devices
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

A chemical sensor includes a piezoelectric support (1) capable of supporting a shear horizontal wave provided on its surface with an electrode (2,3). The sensor is characterized in that the surface of the piezoelectric support (1) including the region bearing the electrode (2,3) is covered by a layer of dielectric material (9) of thickness 0.5 to 20 microns. The piezoelectric support (1) is preferably a single crystal. The dielectric layer (1) may be of, for example, silicon dioxide or a suitable polymer, and preferably has a thickness of between 0.5 and 5 μm.

Claims

1. A chemical sensor comprising:

a piezoelectric support having a surface;

at least two electrodes spaced apart on said surface of said piezoelectric support;

a layer of dielectric material of thickness 0.5 to 20 microns applied to said surface of said piezoelectric support so as to cover at least said electrodes and a region of said surface intermediate said electrodes; and

a layer of specific active reagent immobilized on said layer of dielectric material and at least intermediate said electrodes,

wherein, in use of said sensor, a shear horizontal wave can be supported at said surface of said piezoelectric support.

2. A sensor as claimed in claim 1, wherein said piezoelectric support is a single crystal.

3. A sensor as claimed in claim 2, wherein said piezoelectric support comprises lithium tantalate or quartz.

4. A sensor as claimed in claim 1, which is a two-port waveguide sensor, wherein one of said electrodes is an input electrode and another of said electrodes is an output electrode.

5. A sensor as claimed in claim 4, wherein a metallic grating is provided between said input and output electrodes.

6. A sensor as claimed in claim 1, wherein said electrodes are photolithographically deposited on said surface as elongate regions of metallization transverse to the direction of propagation of a wave along said surface of said support.

7. A sensor as claimed in claim 6, wherein said elongate metallized regions have a width and spacing of between 1 and 40 μ

m.

8. A sensor as claimed in claim 1, wherein the thickness of said electrodes is between 0.1 and 5 μ

m.

9. A sensor as claimed in claim 1, wherein a surface of said piezoelectric support opposite to said surface carrying said electrodes is coated or intimately contacted with a material having a good impedance match with the material of said piezoelectric support and high acoustic absorption.

10. A sensor as claimed in claim 1, wherein said layer of dielectric material is of silicon dioxide.

11. A sensor as claimed in claim 1, wherein said layer of dielectric material is polymeric.

12. A sensor as claimed in claim 1, wherein the thickness of said layer of dielectric material is between 0.5 and 5 μ

m.

13. A sensor as claimed in claim 1, wherein said layer of dielectric material is derivatized or activated to facilitate immobilization thereon of said layer of specific active reagent.