Nucleic acid sequence amplification methods

• US 5,480,784 A
• Filed: 07/10/1990
• Issued: 01/02/1996
• Est. Priority Date: 07/11/1989
• Status: Expired due to Term

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: Kacian, Daniel L., Fultz, Timothy J.
• Primary Examiner(s): Zitomer, Stephanie W.
• Application Number:
  US07/550,837
• Time in Patent Office: 2,002 Days
• Field of Search: 435/6, 435/91, 435/91.2, 435/91.21, 536/27
• US Class Current: 435/91.21
• CPC Class Codes: C12Q 1/6865 Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 1/70 involving virus or bacteriophage immunoassay for viruses G01N33/56983
• CPC Class Codes: C12Q 1/703 Viruses associated with AIDS
• CPC Class Codes: C12Q 1/706 for hepatitis
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. Nucleotide sequences of target nucleic acid portions and of primers are selected to minimize the ability of the primer to remain able to form a DNA primer extension product of for formation of an RNA:DNA primer hybrid and exposure to RNase H.

Claims

1. An improved method of synthesizing multiple copies of an RNA target sequence which comprises:

(a) providing a primer which hybridizes to the 3'"'"' terminal portion of said RNA target sequence, wherein said portion of said RNA target sequence and the primer sequence are selected to maximize the ability of said primer to remain able to form a DNA primer extension product after formation of an RNA;

DNA-primer hybrid and exposure to RNase H activity;

(b) providing a promoter-primer which hybridizes to a portion of said DNA primer extension product, wherein the sequence of said portion of said DNA primer extension product and the primer sequence of said promoter-primer are selected to maximize the ability of said RNase H activity to selectively digest RNA from the 5'"'"' terminal portion of said RNA target sequence present in an RNA;

DNA-primer extension duplex thereby to facilitate hybridization of said promoter-primer; and

,

(c) combining said RNA target sequence with said primer said promoter-primer, reverse transcriptase, said RNase H activity and a transcriptase; and

(d) synthesizing multiple copies of said RNA target sequence.

2. A method according to claim 1 wherein said RNase activity is provided by said reverse transcriptase.

3. A method according to claim 1 where said RNase H activity is provided by RNase H.

4. An improved method of synthesizing multiple copies of an RNA target sequence which comprises:

(a) contacting a nucleic acid comprising said RNA target sequence with a first oligonucleotide which comprises a primer which hybridizes to the 3'"'"' terminal portion of said RNA target sequence under conditions whereby DNA synthesis may be initiated, wherein said 3'"'"' terminal portion of said RNA target sequence and the primer sequence of said first oligonucleotide are selected to maximize the ability of said primer to remain able to form a DNA primer extension product after formation of an RNA;

DNA-primer hybrid and exposure to RNase H activity;

(b) extending the 3'"'"'-terminus of said primer in an extension reaction using said RNA target sequence as a template to give a DNA primer extension product complementary to said RNA target sequence;

(c) selectively digesting the 5'"'"' terminal portion of said RNA target sequence with said RNase H activity

(d) contacting said DNA primer extension product with a second oligonucleotide which comprises a promoter sequence and which has a nucleotide sequence which hybridizes with the 3'"'"' terminal portion of said DNA primer extension product under conditions whereby DNA synthesis may be initiated, wherein the sequence of said 3'"'"'-terminal portion of said DNA primer extension product and said nucleotide sequence of said second oligonucleotide are selected to maximize the ability of said RNase H activity to selectively digest RNA from said 5'"'"' terminal portion of said RNA target sequence present in an RNA;

DNA-primer extension duplex thereby to facilitate hybridization of said second oligonucleotide; and

,

(e) extending the 3'"'"'-terminus of said DNA primer extension product in a DNA extension reaction to produce a template for an RNA polymerase which recognizes the promoter sequence; and

(f) using the template of step (e) to synthesize multiple RNA copies of said target sequence using said RNA polymerase.

5. A method according to claim 4 wherein said RNase H activity is provided by a reverse transcriptase.

6. A method according to claim 4 where said RNase H activity is provided by RNase H.