Use of porous polycrystalline aragonite as a support material for in vitro culture of cells

• US 5,480,827 A
• Filed: 05/03/1993
• Issued: 01/02/1996
• Est. Priority Date: 07/19/1991
• Status: Expired due to Term

• Current Assignee: Bio Holdings International Limited
• Original Assignee: Inoteb Co.
• Inventors: Meunier, Alain, Christel, Pascal, Guillemin, Genevieve, Patat, Jean-Louis
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Larson, Kristin
• Application Number:
  US08/030,134
• Time in Patent Office: 974 Days
• Field of Search: 435/240.23, 435/174, 435/240.243
• US Class Current: 435/395
• CPC Class Codes: C12N 2533/18 Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A three-dimensional solid support for the in vitro cultivation of cells consists essentially of porous polycrystalline aragonite in the form of a cylinder, sphere or plate. The support may be used to successively grow layers of different cells on the support, and is particularly useful for cultivating endothelial cells, fibroblasts and bone marrow cells.

Claims

1. A method for culturing mammalian cells in vitro comprising:

a) immersing a three-dimensional solid support in a liquid cell culture media;

b) inoculating mammalian cells in said medium; and

c) culturing said cells for a time and under conditions to allow growth of said cells on said three-dimensional solid support thereby obtaining a product;

wherein said three-dimensional solid support consists of porous polycrystalline aragonite.

2. The method of claim 1 wherein said mammalian cells are selected from the group consisting of fibroblasts, endothelial cells, bone marrow cells, and osteoblasts.

3. The method of claim 2, wherein said bone marrow cells are totipotent stem cells.

4. The method of claim 1, wherein said mammalian cells are transformed cells.

5. The method of claim 1, wherein said mammalian cells are hybridomas.

6. The method of claim 1, wherein said culturing comprises culturing first cells selected from the group consisting of endothelial cells and fibroblasts on said three-dimensional solid support to form a stratum of said first cells on said support, and subsequently culturing second cells consisting of bone marrow cells on said stratum of said first cells on said support.

7. The method of claim 6, wherein said bone marrow cells are totipotent stem cells or osteoblasts.

8. The product obtained by the method of claim 1.