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Spatially-addressable immobilization of anti-ligands on surfaces

  • US 5,482,867 A
  • Filed: 04/23/1993
  • Issued: 01/09/1996
  • Est. Priority Date: 11/13/1989
  • Status: Expired due to Term
First Claim
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1. A method for forming predefined regions on a surface of a solid support, the predefined regions capable of selectively immobilizing an anti-ligand, the method comprising:

  • a) attaching to the surface a caged biotin analog having a low affinity for (i) the anti-ligand or (ii) a specific binding substance capable of binding an anti-ligand, said caged biotin analog comprising a photolabile chemical protecting group, which chemical protecting group is removable by irradiation to convert said caged biotin analog to a biotin analog capable of non-covalently immobilizing (i) the anti-ligand or (ii) the specific binding substance by an interaction having an affinity constant of 107 or stronger; and

    b) selectively irradiating a predefined region of the surface to convert the caged biotin analogs in the predefined region to the biotin analog capable of non-covalently immobilizing (i) the anti-liqand or (ii) the specific binding substance.

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