Anti-viral methods using RNAse and DNAse

• US 5,484,589 A
• Filed: 03/17/1995
• Issued: 01/16/1996
• Est. Priority Date: 04/20/1992
• Status: Expired due to Fees

• Current Assignee: Rufeld Incorporated
• Original Assignee: Rufeld Incorporated
• Inventors: Salganik, Rudolf I.
• Primary Examiner(s): Knode, Marian C.
• Assistant Examiner(s): Witz, Jean C.
• Application Number:
  US08/407,032
• Time in Patent Office: 305 Days
• Field of Search: 424/94.2, 424/94.6, 435/236, 435/199
• US Class Current: 424/94.2
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 63/10 Animals; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/50 Isolated enzymes; Isolated proteins peptides A01N37/46
• CPC Class Codes: A61K 38/465 acting on ester bonds (3.1), e.g. lipases, ribonucleases

Abstract

Methods for the treatment and prevention of human, animal and plant viral infections are provided. The methods consist of administering to an infected human, animal or plant an effective amount of DNase and RNase or other nuclease exhibiting properties of both enzymes, preferably in conjunction with a suitable divalent cation activator. The effective amount preferably provides a systemic or local concentration between about 5×10^-3 to 10×10^-3 Kunitz units of activity of each nuclease per ml of body fluids and between about 0.1 to 0.3 Kunitz units or more per ml of solution for incubating, growing, soaking or spraying plant seeds, microclones, seedlings or adult plants.

Claims

1. A method for treating a dog infected by a canine viral infection selected from the group consisting of parvovirus and parvomixovirus comprising administering to said infected dog a therapeutically effective amount of DNase and RNase.

2. The method of claim 1 wherein said DNase and RNase is formulated by dissolving 10 mg of DNase and 10 mg of RNase in a sterile solution of 0.005MgCl₂ ;

adding antibiotics to the RNase and DNase solution; and

is administered by intramuscular injection.