Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods

• US 5,484,699 A
• Filed: 09/30/1994
• Issued: 01/16/1996
• Est. Priority Date: 09/28/1990
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Bouma, Stanley R., Joseph, Jeffrey L., Laffler, Thomas G., Marshall, Ronald L.
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): CAMPBELL, EGGERTON A
• Application Number:
  US08/316,293
• Time in Patent Office: 473 Days
• Field of Search: 435/5, 435/6, 935/77, 935/78, 536/23.1, 536/23.72, 536/24.3
• US Class Current: 435/5
• CPC Class Codes: C12Q 1/708 for papilloma

Abstract

Short nucleotide sequences of human papilloma virus useful for the determination of the presence and type of human papilloma virus present in a test sample. The sequences provided can be amplified by polymerase chain reaction or ligase chain reaction. The sequences provided also can be hybridized by standard slot-, dot- or replica-blot procedures. Methods and kits also are provided for the detection of human papilloma virus in a test sample and the determination of the type of human papilloma virus present in the test sample.

Claims

1. A type-specific oligonucleotide for determining the presence of human papilloma virus type 16, having a sequence selected from the group consisting of:

space="preserve" listing-type="tabular">______________________________________ SEQ ID No. ______________________________________ 13 AGGGCGCCAT GAGACTGAAA CAC, 20 ATGACAAATC TTGATACTGC ATCCA, 27 CCATCTGTAC CCTCTACATC TTTAT, 34 GTCTACTGCA AATTTAGCCA GTTCA, 59 AAGTTGTAAG CACGGATGAA TATGT, 60 ACATATTCAT CCGTGCTTAC AACT, 61 TGCACGCACA AACATATATT ATCA, 62 ATGATAATAT ATGTTTGTGC GTGCA, 67 ATTTATACAT TAAAGGCTCT GGGTC, 68 GACCCAGAGC CTTTAATGTA TAAA, 69 TACTGCAAAT TTAGCCAGTT CAAA, 70 ATTTGAACTG GCTAAATTTG CAGTA, 77 AGGCTCTGGG TCTACTGCAA ATTT, 78 AAATTTGCAG TAGACCCAGA GCCT, 81 GCTGCAAACA ACTATACATG ATATAA, 82 TTATATCATG TATAGTTGTT TGCAGC, 83 TATTAGAATG TGTGTACTGC AAGCA, 84 TGCTTGCAGT ACACACATTC TAATA, 93 GTATGGAACA ACATTAGAAC AGCA, 94 TGCTGTTCTA ATGTTGTTCC ATAC, 95 ATACAACAAA CCGTTGTGTG ATTT and 96 AAATCACACA ACGGTTTGTT GTAT;

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and their complements.

2. A method for determining the presence of human papilloma virus type 16 in a test sample, comprising:

a. hybridizing DNA in the test sample with at least one oligonucleotide selected from the group of claim 1, said oligonucleotide being conjugated to a signal generating compound capable of producing a detectable signal; and

b. determining the presence of human papilloma virus by detecting the signal generated.