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Method for the colorimetric determination of an analyte with a PQQ-dependent dehydrogenase

  • US 5,484,708 A
  • Filed: 04/08/1994
  • Issued: 01/16/1996
  • Est. Priority Date: 04/08/1993
  • Status: Expired due to Term
First Claim
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1. A method for the colorimetric determination of an analyte, comprising,enzymatically oxidizing an analyte with an oxidoreductase in the presence of a direct electron acceptor, wherein said direct electron acceptor is an electron-rich aromatic nitroso compound,wherein the electron-rich aromatic nitroso compound is reduced enzymatically in the presence of a pyrrolo-quinoline quinone (PQQ)-dependent dehydrogenase to an imino compound with concomitant oxidation of the analyte without further enzymatic reduction to an aromatic amine,reacting said imino compound with a non-oxidative chromogenic detection reagent, andmeasuring any color formation.

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