Multiwell in-vitro fertilization plate

• US 5,484,731 A
• Filed: 05/26/1993
• Issued: 01/16/1996
• Est. Priority Date: 05/26/1993
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Stevens, Timothy A.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Ludlow, Jan M.
• Application Number:
  US08/067,640
• Time in Patent Office: 965 Days
• Field of Search: 435/297-301, 422/102, 206/569, 206/558, 220/345, 220/346, 220/351, 220/367, 220/374
• US Class Current: 435/305.3
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B65D 43/20 linearly slidable
• CPC Class Codes: C12M 21/06 for in vitro fertilization
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means

Abstract

A plate assembly for useful for in vitro fertilization procuedures such as receiving ova and for forming, evaluating, holding, manipulating and culturing embryos includes a base with an apportioned top surface and a sidewall. The top surface has a plurality of open wells embedded therein for receiving ova to be cultured. The wells are substantially cylindrical and have substantially flat closed bottoms to facilitate microscopic examination of the well contents. The assembly includes a removable lid for covering the top surface of the base. The lid is preferably divided into sections comparable to the top surface portions of the base. Each lid section may be separately slidably moved between a closed position where the lid covers a comparable portion of the top surface and an open position where the top surface is uncovered. The lid has projections which interact with grooves in the base in a cam/cam follower relationship to allow slidable movement of the lid sections between the closed and the open positions while retaining the lid sections to the base. The inside surface of the lid and the base top surface each have elements which interact when the lid is in the closed position to form a tortuous path for gas interchange between the wells and the outside environment, thus substantially preventing microbial contamination of the wells when the lid is closed.

Claims

1. A plate assembly useful for in vitro fertilization procedures comprising:

a base having a top surface divided into top surface portions and a sidewall, said top surface having a plurality of open wells therein, said wells having an inside surface for receiving ova to be cultured into embryos, said wells having substantially flat transparent closed bottoms for facilitating microscopic examination therethrough; and

a removable lid for covering said top surface and at least a portion of said sidewall of said base, said lid being divided into sections, wherein each section of said lid is separately slidably movable between a closed position wherein said each lid section covers said each top surface portion and an open position uncovering said each top surface portion.

2. The assembly of claim 1 wherein said base further includes said wells being substantially cylindrical and having open tops each with a raised lip projecting above said top surface;

and wherein said lid further includes an inside surface having a plurality of raised rings projecting therefrom, said rings corresponding in number to said plurality of said wells in said base portion, said rings being positioned and sized so that when said each lid section is in said closed position, said rings form a substantially concentric barrier adjacent and outside said lips on said wells, thereby providing a tortuous path for gas interchange with said wells.

3. The assembly of claim 2 wherein said base top surface portions have a perimeter defined by a continuous raised shoulder, said lid sections each having a perimeter, said lid sections inside surface each having a raised bead defining said perimeter, said bead being sized and positioned so that when said lid section is in said closed position and said rings are adjacent to said lips, said raised shoulder is positioned substantially adjacent said raised bead, thereby forming a tortuous path for gas interchange and substantially preventing microbial contamination.

4. The assembly of claim 3 wherein each bead further includes a plurality of standoffs projecting therefrom for contacting said shoulder, thereby maintaining a clearance between said shoulder and said bead when said lid sections are in said closed position.

5. The assembly of claim 1 wherein said base top surface is substantially square, having two substantially equal portions, each portion having two substantially equal cylindrical wells substantially symmetrically therein.

6. The assembly of claim 5 wherein said sidewall has a plurality of grooves therein, and said lid sections having inward projections placed and sized to fit in said grooves in a cam/cam follower relationship so that when said lid sections are positioned for covering said wells, said projections are in a first position in said grooves, and when said lid sections are slidably moved to uncover said top surface, said projections are in a second position in said grooves, thereby retaining said lid sections on said base.

7. The assembly of claim 6 wherein said base further includes an extension located on a second portion of said sidewall not covered by said lid sections when lid sections are in said closed position, said extension being sized and shaped for aiding manipulation of said assembly, and said extension having a surface for receiving identification marking.

8. The assembly of claim 7 formed from a plastic resin.

9. The assembly of claim 8 wherein said inside surface of said wells has a treatment selected from the group consisting of plasma treatment and surface oxidation for facilitating cell attachment and growth.

10. A plate assembly for receiving ova, for forming, evaluating, holding, manipulating and culturing embryos comprising:

a base having a sidewall and a substantially square top surface having four substantially equal generally cylindrical wells therein, said wells having an inside surface and closed bottoms, said bottoms being substantially flat and transparent for observing therethrough, each well having an open top and a lip projecting above said top surface, said top surface being divided into two substantially equal portions, said portions each having two wells and a perimeter defined by a raised shoulder; and

a removable lid for covering said top surface of said base and a first portion of said sidewall, said lid being divided into two substantially equal sections, each section separately slidably movable between a closed position wherein said one portion of said top surface is covered and an open position wherein said one portion of said top surface is uncovered, said lid sections each having an interior surface substantially corresponding to said top surface portions, said surfaces of said lid sections each having thereon two substantially circular raised rings and a perimeter defined by a raised bead, said bead and said rings being positioned and sized so that when said lid sections cover said top surface, said rings are outside and adjacent said lips and said bead is adjacent said raised shoulder forming a tortuous path at said perimeter and at said lip of said wells for gas interchange and substantially preventing microbial contamination.

11. The assembly of claim 10 wherein said sidewall has a plurality of grooves in said first portion of said sidewall, and said lid sections each have projections, said projections being placed and sized to fit within and slidably move within said grooves in a cam/cam follower relationship when said lid sections are slidably moved between said closed position and said open position, thereby substantially retaining said lid section on said base.

12. The assembly of claim 11 wherein said base further includes an extension located on a second portion of said sidewall, said second portion not being covered by said lid when said lid is in said closed position, said extension being shaped and sized for aiding manipulation of said assembly and having a surface for receiving identification marking.

13. The assembly of claim 11 wherein said grooves have detents for releasably retaining said projections when said lid sections are in said closed position.

14. The assembly of claim 11 wherein said lid sections have an outside surface, at least a portion of said lid sections outside surface having raised knurling for facilitating gripping and slidably moving said sections.

15. The assembly of claim 11 wherein at least a portion of said second portion of said sidewall of said base includes raised knurling for facilitating gripping and slidably moving said lid sections.

17. The assembly of claim 11 formed from a plastic resin.

16. A packaged plate assembly useful for in vitro fertilization procedures comprising:

a sealed package substantially resistant to the passage of microorganisms having therewithin a plate assembly including a base having a top surface divided into top surface portions and a sidewall, said top surface having a plurality of open wells therein, said wells having an inside surface for receiving ova to be cultured into embryos, said wells having substantially flat transparent closed bottoms for facilitating microscopic examination therethrough; and

a removable lid for covering said top surface and at least a portion at said sidewall, of said base, said lid being divided into sections, wherein each section of said lid is separately slidably moved between a closed position wherein said lid section covers said each top surface portion and an open position uncovering said each top surface portion.