Devices and kits for immunological analysis
First Claim
1. An immunological analysis device consisting of a porous sheet of nitrocellulose containing an array of preselected geometry of delimited absorption areas of at least one compound capable of specifically binding an antigen (antigen-reactive compound) which adheres tightly and does not spread out on the surface of said porous sheet, said array of preselected geometry being obtained in said porous sheet by applying liquid aliquots of said antigen-reactive compound to said porous sheet mechanically via direct contact, wherein residual absorption sited in said porous sheet are unsaturated or saturated by non-specific protein.
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Accused Products
Abstract
New devices and kits for solid-phase immuno-assays comprising a solid porous support, preferably in the form of a sheet, where antigens or immuno-globulins or both of them are bound by direct application in any suitable geometry, e.g. as an assay of dots or lines. Such porous supports are suitable for effecting an unlimited number of antibody-antigen reactions simultaneously and in one operation.
268 Citations
47 Claims
- 1. An immunological analysis device consisting of a porous sheet of nitrocellulose containing an array of preselected geometry of delimited absorption areas of at least one compound capable of specifically binding an antigen (antigen-reactive compound) which adheres tightly and does not spread out on the surface of said porous sheet, said array of preselected geometry being obtained in said porous sheet by applying liquid aliquots of said antigen-reactive compound to said porous sheet mechanically via direct contact, wherein residual absorption sited in said porous sheet are unsaturated or saturated by non-specific protein.
- 26. An immunological analysis device consisting of a porous sheet of nitrocellulose containing an array of preselected geometry of delimited absorption areas of at least one antigen, which adheres tightly and does not spread out on the surface of said porous sheet, said array of preselected geometry being obtained in said porous sheet by applying liquid aliquots of said antigen to said porous sheet mechanically via direct contact, wherein residual absorption sites in said porous sheet are unsaturated or saturated by non-specific protein.
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47. A method of producing an immunoassay device which comprises the steps of:
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(a) applying droplets of substantially enriched aqueous solutions of antibodies of distinct specification to a substantially planar surface of a support, locating said droplets so that they cover small, discrete, closely spaced areas of the planar surface of the support and form an array; (b) allowing said aqueous solutions of antibodies to remain on said areas of the surface for a period of time long enough for the antibodies in solution to be adsorbed to said areas of the surface to form discrete, antibody-coated areas; (c) swiftly flooding the entire surface of the solid support with an aqueous solution of protein in order to block non-specific binding sites in areas of the surface of the solid support which surround the antibody-coated areas; and (d) washing and drying the device.
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Specification