Determining material concentrations in tissues

• US 5,492,118 A
• Filed: 06/03/1994
• Issued: 02/20/1996
• Est. Priority Date: 12/16/1993
• Status: Expired due to Term

• Current Assignee: Board of Trustees of The University of Illinois
• Original Assignee: Board of Trustees of The University of Illinois
• Inventors: Maier, John, Walker, Scott A., Franceschini, Maria A., Fantini, Sergio, Gratton, Enrico
• Primary Examiner(s): Sykes, Angela D.
• Assistant Examiner(s): NASSER, ROBERT L
• Application Number:
  US08/253,935
• Time in Patent Office: 627 Days
• Field of Search: 128/633-634, 128/664-666, 250/341.1, 250/341.3, 250/343, 250/339.1, 356/41, 356/338, 356/446
• US Class Current: 600/316
• CPC Class Codes: A61B 2562/0233 Special features of optical sensors or probes classified in A61B5/00
• CPC Class Codes: A61B 2562/046 in a matrix array
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/14552 Details of sensors specially adapted therefor
• CPC Class Codes: A61B 5/6824 Arm or wrist
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

The relative concentration of a material such as glucose in a turbid medium such as living tissue may determining the scattering coefficient of the light that has passed through the turbid medium; and comparing the scattering coefficient with a previous scattering coefficient determined with respect to the turbid medium.

Claims

1. A method of determining the relative concentration of a material in a turbid medium which comprises:

shining light through said turbid medium;

determining the scattering coefficient of said turbid medium using said light that has passed through the turbid medium;

comparing said scattering coefficient with a previous scattering coefficient of said turbid medium, whereby a change in said scattering coefficient indicates a change in the relative concentration of said material; and

determining said relative concentration from said scattering coefficients.

2. The method of claim 1 in which said turbid medium is living, cellular tissue.

3. The method of claim 1 in which said turbid medium is an emulsion.

4. The method of claim 1 in which the wavelength of said light is from about 650 nm to about 1,300 nm.

5. The method of claim 1 in which said light shining through said turbid medium has an intensity and is provided by sequentially illuminating and shutting off a first group of light sources which each emit light of a first wavelength and which are spaced at different distances from a light sensor, while modulating the intensity of said light from said light sources at a first frequency, and in which a signal at a second frequency, coherent with said modulated light, is provided to said light sensor.

6. The method of claim 5 comprising modulating the gain of, or multiplying the output of, said light sensor by said coherent signal, said second frequency being different from said first frequency, and further deriving a resultant signal from the sensor while sequentially receiving said modulated light from said plurality of light sources through said turbid medium, said resultant signal being at a frequency of the difference between the first and second frequencies, to detect at least two of phase shift, DC, and AC components of said modulated light as sensed by the sensor, compared with the modulated light as originally emitted by the light sources.

7. The method of claim 6 comprising sequentially illuminating each of said plurality of light sources for a length of time that is an exact multiple of a wave period having a frequency which comprises the difference between said first and second frequencies.

8. The method of claim 7 comprising summing and averaging information sensed by said light sensor from each light source from about 8 to about 800 times to obtain an intensified average of the photometric information received from each light source.

9. The method of claim 8, wherein said shining step further comprises illuminating a second group of light sources which each emit light of a second different wavelength.

10. The method of claim 6, wherein said shining step further comprises illuminating a second group of light sources which each emit light of a second different wavelength.

11. The method of claim 1 in which said material is glucose.

12. A method of determining a tissue component concentration in a patient without drawing blood, which comprises placing an optical sensor head on the skin of the patient, said head carrying a first group of light sources and a light sensor, at least some of said light sources being at differing distances from said sensor and emitting light of a first wavelength, whereby pressing of said sensor against the skin permits the sensor to sense exclusively light from said light sources passing through tissue of the patient;

passing light from said light sources in sequential manner through the tissue of said patient to the light sensor;

determining the scattering coefficient of said tissue from said light that is passed through the tissue of said patient;

comparing said scattering coefficient with a scattering coefficient previously obtained from the same portion of tissue, to obtain a relative concentration of said component of said tissue; and

determining said relative concentration from said scattering coefficients.

13. The method of claim 12 in which the wavelength of said light is from about 650 nm to about 1,300 nm.

14. The method of claim 13 in which said light shining through said tissue has an intensity and is provided by sequentially illuminating and shutting off the first group of light sources which are spaced at different distances from a light sensor while modulating the intensity of said light from said light sources at a first frequency, and in which a signal at a second frequency, coherent with said modulated light, is provided to said light sensor.

15. The method of claim 14 comprising modulating the gain of, or multiplying the output of, said light sensor by said coherent signal of second frequency, said second frequency being different from said first frequency, and further deriving resultant signals from the sensor while sequentially receiving said modulated light from said first group of light sources through said tissue, said resultant signals being at a frequency of the difference between the first and second frequencies, to determine at least two of phase shift, DC, and AC components of said modulated light as sensed by the sensor, compared with the modulated light as originally emitted by the light sources.

16. The method of claim 15 comprising sequentially illuminating each of said first group of light sources for a length of time that is an exact multiple of a wave period having a frequency which comprises the difference between said first and second frequencies.

17. The method of claim 16 comprising summing and averaging information sensed by said light sensor from each light source from about 8 to about 800 times to obtain an intensified average of the photometric information received from each light source.

18. The method of claim 17, wherein said passing step further comprises illuminating a second group of light sources which each emit light of a second different wavelength.

19. The method of claim 12 in which said tissue component is glucose.

20. A method of determining the relative concentration of glucose in living cellular tissue which comprises:

shining light through said tissue for a first time and thereafter shining light through said tissue a second time;

determining the scattering coefficients of said light which is passed through said tissue during the first and second times, the wavelength of said light being substantially constant and from about 650 nm to about 1300 nm;

comparing the scattering coefficients, whereby a difference in said scattering coefficients indicates a change in the relative concentration of glucose present in said tissue; and

determining said relative concentration from said scattering coefficients.

21. The method of claim 20 in which said light shining through said tissue has an intensity, and is provided by sequentially illuminating and shutting off a plurality of light sources, said light sources being spaced at different distances from a light sensor which senses light from said sources scattered in said tissue.

22. The method of claim 21 including the step of modulating the intensity of the light from said light sources at a first frequency, and in which a signal at a second frequency, coherent with said modulated light, is provided to said light sensor to modulate the gain or the output of said light sensor by said coherent signal, said second frequency being different from said first frequency, and further deriving a resultant signal from the sensor while sequentially receiving said modulated light by the sensor from said plurality of light sources through said tissue, said resultant signal being at a frequency of the difference between the first and second frequencies, to detect at least two of phase shift, DC, and AC components of said modulated light as sensed by the sensor.

23. The method of claim 22 in which a first group of said light sources emits light of a first wavelength, and in which a second group of said light sources emits light of a second, different wavelength, the light sources of each of said first group and said second group being spaced by differing distances from said sensor when compared with other light sources of its group.

24. A method of determining changing conditions in living tissue, which comprises:

placing an optical sensor head on the skin of a patient, said head carrying a plurality of light sources and a light sensor, at least some of the light sources being at differing distances from the sensor and emitting light of essentially the same wavelength;

sequentially activating and shutting off said light sources to cause the sensor to sequentially sense light from said sources passing through said tissue;

determining the scattering coefficient from said light that has passed through said tissue;

comparing said scattering coefficient with a previous scattering coefficient of said tissue; and

determining said changing conditions from said scattering coefficients.

25. The method of claim 24 in which said changing conditions comprise changes in the concentration of a tissue component.