Method for the production of double-haploid cucumbers

• US 5,492,827 A
• Filed: 01/11/1995
• Issued: 02/20/1996
• Est. Priority Date: 12/22/1988
• Status: Expired due to Fees

• Current Assignee: Nunhems Zaden BV (Bayer AG)
• Original Assignee: Nunhems Zaden BV (Bayer AG)
• Inventors: Dirks, Robert
• Primary Examiner(s): Fox, David T.
• Assistant Examiner(s): McElwain, Elizabeth F.
• Application Number:
  US08/371,464
• Time in Patent Office: 405 Days
• Field of Search: 800/200, 800/DIG. 18, 435/240.4, 435/240.49, 435/240.45, 47/58.05, 47/58, 47/58.03
• US Class Current: 435/430.1
• CPC Class Codes: A01H 1/08 Methods for producing changes in chromosome number

Abstract

Obtaining double-haploid cucumber plants from haploid plants is considerably improved when immature or unfertilized ovulae or embryo sac cells, which may be contained in ovarial tissue, are isolated, the formation of callus, embryos or shoots is induced on a hormone-containing medium, these haploid plants are cultured and duplication of the genome is effected.

Claims

1. A method for producing haploid Cucumis sativus plants which comprises isolating a female flower part selected from the group consisting of unfertilized ovules, embryo sac cells, ovarial tissue containing unfertilized ovules and ovarial tissue containing embryo sac cells, inducing the direct formation of embryos with hormone-containing medium, culturing the embryos to give haploid plants;

wherein when the female flower part is from a parthenocarpous plant, the medium contains cytokinins and, when the female flower part is from a non-parthenocarpous plant, the medium contains auxins.

2. The method as claimed in claim 1, wherein a Miller medium containing inorganic salts is used as the medium.

3. The method of claim 1 wherein the female flower part is contained in fruit tissue.

4. The method of claim 3 wherein a Miller medium containing inorganic salts is used as the medium.

5. The method of claim 1 wherein the isolating is under substantially aseptic conditions.

6. The method of claim 1 wherein the culturing is at a temperature between about 20°

and about 30°

C.

7. The method of claim 6 wherein the culturing is at a temperature of about 27°

to about 28°

C.