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Surface plasmon resonance sensor unit and its use in biosensor systems

  • US 5,492,840 A
  • Filed: 05/10/1991
  • Issued: 02/20/1996
  • Est. Priority Date: 11/10/1988
  • Status: Expired due to Term
First Claim
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1. A sensor unit for use in a biosensor system based on surface plasmon resonance technology, comprising:

  • a dielectric substrate which is replaceable in said biosensor system and which is coated with a metal film, said film having two or more sensing areas arranged so as to be passed in series or in parallel by a liquid stream,each of said sensing areas comprising a layer of an organic polymer or hydrogel over said metal film, containing at least one functional group supporting bi- or polyfunctional molecules capable of specific binding with biomolecules present in said liquid stream which are to be detected, said bi- or polyfunctional molecules comprising ligands that can specifically bind to biomolecules in a sample and a functional structure which can form reversible bonds with said at least one functional group which, through said functional structure, are coupled to said sensing area by a reversible bond, such that said two or more sensing areas can be regenerated for refunctionalization with the same or other bi- or polyfunctional molecules, whereincoupling between said bi- or polyfunctional molecules and said biomolecules in said liquid stream is selectively reversible such that the bi- or polyfunctional molecule-functionalized sensing areas can be regenerated, permitting repeated use of the functionalized sensor unit.

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