Surface plasmon resonance sensor unit and its use in biosensor systems
First Claim
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1. A sensor unit for use in a biosensor system based on surface plasmon resonance technology, comprising:
- a dielectric substrate which is replaceable in said biosensor system and which is coated with a metal film, said film having two or more sensing areas arranged so as to be passed in series or in parallel by a liquid stream,each of said sensing areas comprising a layer of an organic polymer or hydrogel over said metal film, containing at least one functional group supporting bi- or polyfunctional molecules capable of specific binding with biomolecules present in said liquid stream which are to be detected, said bi- or polyfunctional molecules comprising ligands that can specifically bind to biomolecules in a sample and a functional structure which can form reversible bonds with said at least one functional group which, through said functional structure, are coupled to said sensing area by a reversible bond, such that said two or more sensing areas can be regenerated for refunctionalization with the same or other bi- or polyfunctional molecules, whereincoupling between said bi- or polyfunctional molecules and said biomolecules in said liquid stream is selectively reversible such that the bi- or polyfunctional molecule-functionalized sensing areas can be regenerated, permitting repeated use of the functionalized sensor unit.
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Abstract
Methods for functionalizing sensing surfaces to be used in systems for measuring simultaneously several properties of one biomolecule as well as for simultaneously measuring the concentrations of a plurality of biomolecules in a sample. The invention furthermore also relates to sensor units containing such surfaces, to the use thereof for surface characterization of biomolecules, and to a reagent kit for functionalization of sensing surfaces.
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Citations
23 Claims
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1. A sensor unit for use in a biosensor system based on surface plasmon resonance technology, comprising:
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a dielectric substrate which is replaceable in said biosensor system and which is coated with a metal film, said film having two or more sensing areas arranged so as to be passed in series or in parallel by a liquid stream, each of said sensing areas comprising a layer of an organic polymer or hydrogel over said metal film, containing at least one functional group supporting bi- or polyfunctional molecules capable of specific binding with biomolecules present in said liquid stream which are to be detected, said bi- or polyfunctional molecules comprising ligands that can specifically bind to biomolecules in a sample and a functional structure which can form reversible bonds with said at least one functional group which, through said functional structure, are coupled to said sensing area by a reversible bond, such that said two or more sensing areas can be regenerated for refunctionalization with the same or other bi- or polyfunctional molecules, wherein coupling between said bi- or polyfunctional molecules and said biomolecules in said liquid stream is selectively reversible such that the bi- or polyfunctional molecule-functionalized sensing areas can be regenerated, permitting repeated use of the functionalized sensor unit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification