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Biosensor and method of quantitative analysis using the same

  • US 5,496,453 A
  • Filed: 10/12/1994
  • Issued: 03/05/1996
  • Est. Priority Date: 05/17/1991
  • Status: Expired due to Term
First Claim
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1. A method of quantitative analysis of a specific compound in a liquid sample comprising supplying said liquid sample to a biosensor wherein said biosensor comprises an electrochemical means for detecting the product of a reaction of said specific compound with a biologically active material, and wherein said biologically active material is located at a position remote from the electrochemical detecting means, reading at least two electrochemical signals from the biosensor, which signals are at least a first electrochemical signal produced at the supply of the sample and generated by an electrochemically active substance present in the sample but not a product of a reaction of said specific substance with said biologically active material, and at least a second electrochemical signal generated later than said first signal by a product of a reaction of said specific compound with said biologically active material, and operating both signals, whereby the amount of said specific substance is quantitatively measured.

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